DETEKSI MENGGUNAKAN PCR (POLYMERASE CHAIN REACTION) CANDIDATUS LIBERIBACTER ASIATICUS, PENYEBAB HUANGLONGBING PADA JERUK SIEM DENGAN BEBERAPA TIPE GEJALA PADA DAUN
Detection using PCR (Polymerase Chain Reaction) Candidatus Liberibacter asiaticus, Huanglongbing causal Organism on Siem Mandarin with different types of symptoms. Huanglongbing (HLB) or Citrus Vein Phloem Degeration (CVPD) is one of major diseases on Siem mandarin in Indonesia. HLB is caused by ba...
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| Format: | Article |
| Language: | English |
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Universitas Lampung
2011-11-01
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| Series: | Jurnal Hama dan Penyakit Tumbuhan Tropika |
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| Online Access: | http://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/211 |
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| author | Achmad Himawan, Yohanes Berchmans umardiyono, Susamto Somowiyarjo, Yohanes Andi Trisyono & Andrew Beattie. |
| author_facet | Achmad Himawan, Yohanes Berchmans umardiyono, Susamto Somowiyarjo, Yohanes Andi Trisyono & Andrew Beattie. |
| author_sort | Achmad Himawan, Yohanes Berchmans umardiyono, Susamto Somowiyarjo, Yohanes Andi Trisyono & Andrew Beattie. |
| collection | DOAJ |
| description | Detection using PCR (Polymerase Chain Reaction) Candidatus Liberibacter asiaticus, Huanglongbing causal Organism on Siem Mandarin with different types of symptoms. Huanglongbing (HLB) or Citrus Vein Phloem Degeration (CVPD) is one of major diseases on Siem mandarin in Indonesia. HLB is caused by bacteria Candidatus liberibacter asiatus (LAS). The bacteria only live in the phloem cells of host tree and only recently it was reported to be successfully cultured on agar medium. Early detection method of LAS is needed to support healthy Siem mandarin cultivation program. This research was conducted to detect LAS in different types of HLB leaf symptoms based on Polymerase Chain Reaction (PCR) method with specific primer forward MHO 353 and reverse MHO 354. The results suggested that 8 types of HLB leaf symptoms were found on the samples used in this experiment. LAS was detected at 60% on the leaves without any symptom followed by the leaves with completely chlorosis symptom at 66%. The leaves with unevenly yellow showing higher percentage of LAS detection ranged from 80-86%. PCR technique successfully amplified DNA of LAS with the size target of 600 bp. |
| first_indexed | 2024-04-13T21:07:04Z |
| format | Article |
| id | doaj.art-d70d1c737733481fa81d404cec7d7857 |
| institution | Directory Open Access Journal |
| issn | 1411-7525 2461-0399 |
| language | English |
| last_indexed | 2024-04-13T21:07:04Z |
| publishDate | 2011-11-01 |
| publisher | Universitas Lampung |
| record_format | Article |
| series | Jurnal Hama dan Penyakit Tumbuhan Tropika |
| spelling | doaj.art-d70d1c737733481fa81d404cec7d78572022-12-22T02:29:57ZengUniversitas LampungJurnal Hama dan Penyakit Tumbuhan Tropika1411-75252461-03992011-11-0110217818310.23960/j.hptt.210178-183195DETEKSI MENGGUNAKAN PCR (POLYMERASE CHAIN REACTION) CANDIDATUS LIBERIBACTER ASIATICUS, PENYEBAB HUANGLONGBING PADA JERUK SIEM DENGAN BEBERAPA TIPE GEJALA PADA DAUNAchmad Himawan, Yohanes Berchmans umardiyono, Susamto Somowiyarjo, Yohanes Andi Trisyono & Andrew Beattie.Detection using PCR (Polymerase Chain Reaction) Candidatus Liberibacter asiaticus, Huanglongbing causal Organism on Siem Mandarin with different types of symptoms. Huanglongbing (HLB) or Citrus Vein Phloem Degeration (CVPD) is one of major diseases on Siem mandarin in Indonesia. HLB is caused by bacteria Candidatus liberibacter asiatus (LAS). The bacteria only live in the phloem cells of host tree and only recently it was reported to be successfully cultured on agar medium. Early detection method of LAS is needed to support healthy Siem mandarin cultivation program. This research was conducted to detect LAS in different types of HLB leaf symptoms based on Polymerase Chain Reaction (PCR) method with specific primer forward MHO 353 and reverse MHO 354. The results suggested that 8 types of HLB leaf symptoms were found on the samples used in this experiment. LAS was detected at 60% on the leaves without any symptom followed by the leaves with completely chlorosis symptom at 66%. The leaves with unevenly yellow showing higher percentage of LAS detection ranged from 80-86%. PCR technique successfully amplified DNA of LAS with the size target of 600 bp.http://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/211Huanglongbing (HLB), Siem mandarin, Candidatus Liberibacter asiaticus, PCR |
| spellingShingle | Achmad Himawan, Yohanes Berchmans umardiyono, Susamto Somowiyarjo, Yohanes Andi Trisyono & Andrew Beattie. DETEKSI MENGGUNAKAN PCR (POLYMERASE CHAIN REACTION) CANDIDATUS LIBERIBACTER ASIATICUS, PENYEBAB HUANGLONGBING PADA JERUK SIEM DENGAN BEBERAPA TIPE GEJALA PADA DAUN Jurnal Hama dan Penyakit Tumbuhan Tropika Huanglongbing (HLB), Siem mandarin, Candidatus Liberibacter asiaticus, PCR |
| title | DETEKSI MENGGUNAKAN PCR (POLYMERASE CHAIN REACTION) CANDIDATUS LIBERIBACTER ASIATICUS, PENYEBAB HUANGLONGBING PADA JERUK SIEM DENGAN BEBERAPA TIPE GEJALA PADA DAUN |
| title_full | DETEKSI MENGGUNAKAN PCR (POLYMERASE CHAIN REACTION) CANDIDATUS LIBERIBACTER ASIATICUS, PENYEBAB HUANGLONGBING PADA JERUK SIEM DENGAN BEBERAPA TIPE GEJALA PADA DAUN |
| title_fullStr | DETEKSI MENGGUNAKAN PCR (POLYMERASE CHAIN REACTION) CANDIDATUS LIBERIBACTER ASIATICUS, PENYEBAB HUANGLONGBING PADA JERUK SIEM DENGAN BEBERAPA TIPE GEJALA PADA DAUN |
| title_full_unstemmed | DETEKSI MENGGUNAKAN PCR (POLYMERASE CHAIN REACTION) CANDIDATUS LIBERIBACTER ASIATICUS, PENYEBAB HUANGLONGBING PADA JERUK SIEM DENGAN BEBERAPA TIPE GEJALA PADA DAUN |
| title_short | DETEKSI MENGGUNAKAN PCR (POLYMERASE CHAIN REACTION) CANDIDATUS LIBERIBACTER ASIATICUS, PENYEBAB HUANGLONGBING PADA JERUK SIEM DENGAN BEBERAPA TIPE GEJALA PADA DAUN |
| title_sort | deteksi menggunakan pcr polymerase chain reaction candidatus liberibacter asiaticus penyebab huanglongbing pada jeruk siem dengan beberapa tipe gejala pada daun |
| topic | Huanglongbing (HLB), Siem mandarin, Candidatus Liberibacter asiaticus, PCR |
| url | http://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/view/211 |
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