Molecular signatures and cellular diversity during mouse habenula development
Summary: The habenula plays a key role in various motivated and pathological behaviors and is composed of molecularly distinct neuron subtypes. Despite progress in identifying mature habenula neuron subtypes, how these subtypes develop and organize into functional brain circuits remains largely unkn...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-07-01
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| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124722008233 |
| _version_ | 1818535609814220800 |
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| author | Lieke L. van de Haar Danai Riga Juliska E. Boer Oxana Garritsen Youri Adolfs Thomas E. Sieburgh Roland E. van Dijk Kyoko Watanabe Nicky C.H. van Kronenburg Mark H. Broekhoven Danielle Posthuma Frank J. Meye Onur Basak R. Jeroen Pasterkamp |
| author_facet | Lieke L. van de Haar Danai Riga Juliska E. Boer Oxana Garritsen Youri Adolfs Thomas E. Sieburgh Roland E. van Dijk Kyoko Watanabe Nicky C.H. van Kronenburg Mark H. Broekhoven Danielle Posthuma Frank J. Meye Onur Basak R. Jeroen Pasterkamp |
| author_sort | Lieke L. van de Haar |
| collection | DOAJ |
| description | Summary: The habenula plays a key role in various motivated and pathological behaviors and is composed of molecularly distinct neuron subtypes. Despite progress in identifying mature habenula neuron subtypes, how these subtypes develop and organize into functional brain circuits remains largely unknown. Here, we performed single-cell transcriptional profiling of mouse habenular neurons at critical developmental stages, instructed by detailed three-dimensional anatomical data. Our data reveal cellular and molecular trajectories during embryonic and postnatal development, leading to different habenular subtypes. Further, based on this analysis, our work establishes the distinctive functional properties and projection target of a subtype of Cartpt+ habenula neurons. Finally, we show how comparison of single-cell transcriptional profiles and GWAS data links specific developing habenular subtypes to psychiatric disease. Together, our study begins to dissect the mechanisms underlying habenula neuron subtype-specific development and creates a framework for further interrogation of habenular development in normal and disease states. |
| first_indexed | 2024-12-11T18:27:11Z |
| format | Article |
| id | doaj.art-d70ef2f462ea4a0db97ad24431145f38 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-11T18:27:11Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-d70ef2f462ea4a0db97ad24431145f382022-12-22T00:55:01ZengElsevierCell Reports2211-12472022-07-01401111029Molecular signatures and cellular diversity during mouse habenula developmentLieke L. van de Haar0Danai Riga1Juliska E. Boer2Oxana Garritsen3Youri Adolfs4Thomas E. Sieburgh5Roland E. van Dijk6Kyoko Watanabe7Nicky C.H. van Kronenburg8Mark H. Broekhoven9Danielle Posthuma10Frank J. Meye11Onur Basak12R. Jeroen Pasterkamp13Department of Translational Neuroscience, UMC Utrecht Brain Center, University Medical Center, Utrecht University, 3584 Utrecht, the NetherlandsDepartment of Translational Neuroscience, UMC Utrecht Brain Center, University Medical Center, Utrecht University, 3584 Utrecht, the NetherlandsDepartment of Translational Neuroscience, UMC Utrecht Brain Center, University Medical Center, Utrecht University, 3584 Utrecht, the NetherlandsDepartment of Translational Neuroscience, UMC Utrecht Brain Center, University Medical Center, Utrecht University, 3584 Utrecht, the NetherlandsDepartment of Translational Neuroscience, UMC Utrecht Brain Center, University Medical Center, Utrecht University, 3584 Utrecht, the NetherlandsDepartment of Translational Neuroscience, UMC Utrecht Brain Center, University Medical Center, Utrecht University, 3584 Utrecht, the NetherlandsDepartment of Translational Neuroscience, UMC Utrecht Brain Center, University Medical Center, Utrecht University, 3584 Utrecht, the NetherlandsDepartment of Complex Trait Genetics, Center for Neurogenomics and Cognitive Research, Neuroscience Campus Amsterdam, VU University Amsterdam, 1081 Amsterdam, the NetherlandsDepartment of Translational Neuroscience, UMC Utrecht Brain Center, University Medical Center, Utrecht University, 3584 Utrecht, the NetherlandsDepartment of Translational Neuroscience, UMC Utrecht Brain Center, University Medical Center, Utrecht University, 3584 Utrecht, the NetherlandsDepartment of Complex Trait Genetics, Center for Neurogenomics and Cognitive Research, Neuroscience Campus Amsterdam, VU University Amsterdam, 1081 Amsterdam, the NetherlandsDepartment of Translational Neuroscience, UMC Utrecht Brain Center, University Medical Center, Utrecht University, 3584 Utrecht, the NetherlandsDepartment of Translational Neuroscience, UMC Utrecht Brain Center, University Medical Center, Utrecht University, 3584 Utrecht, the NetherlandsDepartment of Translational Neuroscience, UMC Utrecht Brain Center, University Medical Center, Utrecht University, 3584 Utrecht, the Netherlands; Corresponding authorSummary: The habenula plays a key role in various motivated and pathological behaviors and is composed of molecularly distinct neuron subtypes. Despite progress in identifying mature habenula neuron subtypes, how these subtypes develop and organize into functional brain circuits remains largely unknown. Here, we performed single-cell transcriptional profiling of mouse habenular neurons at critical developmental stages, instructed by detailed three-dimensional anatomical data. Our data reveal cellular and molecular trajectories during embryonic and postnatal development, leading to different habenular subtypes. Further, based on this analysis, our work establishes the distinctive functional properties and projection target of a subtype of Cartpt+ habenula neurons. Finally, we show how comparison of single-cell transcriptional profiles and GWAS data links specific developing habenular subtypes to psychiatric disease. Together, our study begins to dissect the mechanisms underlying habenula neuron subtype-specific development and creates a framework for further interrogation of habenular development in normal and disease states.http://www.sciencedirect.com/science/article/pii/S2211124722008233CP: Neuroscience |
| spellingShingle | Lieke L. van de Haar Danai Riga Juliska E. Boer Oxana Garritsen Youri Adolfs Thomas E. Sieburgh Roland E. van Dijk Kyoko Watanabe Nicky C.H. van Kronenburg Mark H. Broekhoven Danielle Posthuma Frank J. Meye Onur Basak R. Jeroen Pasterkamp Molecular signatures and cellular diversity during mouse habenula development Cell Reports CP: Neuroscience |
| title | Molecular signatures and cellular diversity during mouse habenula development |
| title_full | Molecular signatures and cellular diversity during mouse habenula development |
| title_fullStr | Molecular signatures and cellular diversity during mouse habenula development |
| title_full_unstemmed | Molecular signatures and cellular diversity during mouse habenula development |
| title_short | Molecular signatures and cellular diversity during mouse habenula development |
| title_sort | molecular signatures and cellular diversity during mouse habenula development |
| topic | CP: Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2211124722008233 |
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