cGMP-dependent protein kinase I is crucial for angiogenesis and postnatal vasculogenesis.
BACKGROUND: Endothelium-derived nitric oxide plays an important role for the bone marrow microenvironment. Since several important effects of nitric oxide are mediated by cGMP-dependent pathways, we investigated the role of the cGMP downstream effector cGMP-dependent protein kinase I (cGKI) on postn...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2009-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2654072?pdf=render |
| _version_ | 1828385534316118016 |
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| author | Alexandra Aicher Christopher Heeschen Susanne Feil Franz Hofmann Michael E Mendelsohn Robert Feil Stefanie Dimmeler |
| author_facet | Alexandra Aicher Christopher Heeschen Susanne Feil Franz Hofmann Michael E Mendelsohn Robert Feil Stefanie Dimmeler |
| author_sort | Alexandra Aicher |
| collection | DOAJ |
| description | BACKGROUND: Endothelium-derived nitric oxide plays an important role for the bone marrow microenvironment. Since several important effects of nitric oxide are mediated by cGMP-dependent pathways, we investigated the role of the cGMP downstream effector cGMP-dependent protein kinase I (cGKI) on postnatal neovascularization. METHODOLOGY/PRINCIPAL FINDINGS: In a disc neovascularization model, cGKI(-/-) mice showed an impaired neovascularization as compared to their wild-type (WT) littermates. Infusion of WT, but not cGKI(-/-) bone marrow progenitors rescued the impaired ingrowth of new vessels in cGKI-deficient mice. Bone marrow progenitors from cGKI(-/-) mice showed reduced proliferation and survival rates. In addition, we used cGKIalpha leucine zipper mutant (LZM) mice as model for cGKI deficiency. LZM mice harbor a mutation in the cGKIalpha leucine zipper that prevents interaction with downstream signaling molecules. Consistently, LZM mice exhibited reduced numbers of vasculogenic progenitors and impaired neovascularization following hindlimb ischemia compared to WT mice. CONCLUSIONS/SIGNIFICANCE: Our findings demonstrate that the cGMP-cGKI pathway is critical for postnatal neovascularization and establish a new role for cGKI in vasculogenesis, which is mediated by bone marrow-derived progenitors. |
| first_indexed | 2024-12-10T05:21:46Z |
| format | Article |
| id | doaj.art-d7112d0dbd354324b2c8600e2a84805c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T05:21:46Z |
| publishDate | 2009-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d7112d0dbd354324b2c8600e2a84805c2022-12-22T02:00:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032009-01-0143e487910.1371/journal.pone.0004879cGMP-dependent protein kinase I is crucial for angiogenesis and postnatal vasculogenesis.Alexandra AicherChristopher HeeschenSusanne FeilFranz HofmannMichael E MendelsohnRobert FeilStefanie DimmelerBACKGROUND: Endothelium-derived nitric oxide plays an important role for the bone marrow microenvironment. Since several important effects of nitric oxide are mediated by cGMP-dependent pathways, we investigated the role of the cGMP downstream effector cGMP-dependent protein kinase I (cGKI) on postnatal neovascularization. METHODOLOGY/PRINCIPAL FINDINGS: In a disc neovascularization model, cGKI(-/-) mice showed an impaired neovascularization as compared to their wild-type (WT) littermates. Infusion of WT, but not cGKI(-/-) bone marrow progenitors rescued the impaired ingrowth of new vessels in cGKI-deficient mice. Bone marrow progenitors from cGKI(-/-) mice showed reduced proliferation and survival rates. In addition, we used cGKIalpha leucine zipper mutant (LZM) mice as model for cGKI deficiency. LZM mice harbor a mutation in the cGKIalpha leucine zipper that prevents interaction with downstream signaling molecules. Consistently, LZM mice exhibited reduced numbers of vasculogenic progenitors and impaired neovascularization following hindlimb ischemia compared to WT mice. CONCLUSIONS/SIGNIFICANCE: Our findings demonstrate that the cGMP-cGKI pathway is critical for postnatal neovascularization and establish a new role for cGKI in vasculogenesis, which is mediated by bone marrow-derived progenitors.http://europepmc.org/articles/PMC2654072?pdf=render |
| spellingShingle | Alexandra Aicher Christopher Heeschen Susanne Feil Franz Hofmann Michael E Mendelsohn Robert Feil Stefanie Dimmeler cGMP-dependent protein kinase I is crucial for angiogenesis and postnatal vasculogenesis. PLoS ONE |
| title | cGMP-dependent protein kinase I is crucial for angiogenesis and postnatal vasculogenesis. |
| title_full | cGMP-dependent protein kinase I is crucial for angiogenesis and postnatal vasculogenesis. |
| title_fullStr | cGMP-dependent protein kinase I is crucial for angiogenesis and postnatal vasculogenesis. |
| title_full_unstemmed | cGMP-dependent protein kinase I is crucial for angiogenesis and postnatal vasculogenesis. |
| title_short | cGMP-dependent protein kinase I is crucial for angiogenesis and postnatal vasculogenesis. |
| title_sort | cgmp dependent protein kinase i is crucial for angiogenesis and postnatal vasculogenesis |
| url | http://europepmc.org/articles/PMC2654072?pdf=render |
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