Integrative Analysis of miRNA-mRNA in Ovarian Granulosa Cells Treated with Kisspeptin in Tan Sheep
Kisspeptin is a peptide hormone encoded by the kiss-1 gene that regulates animal reproduction. Our studies revealed that kisspeptin can regulate steroid hormone production and promote cell proliferation in ovarian granulosa cells of Tan sheep, but the mechanism has not yet been fully understood. We...
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MDPI AG
2022-10-01
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| author | Tianshu Dai Xiaolong Kang Chaoyun Yang Shan Mei Shihao Wei Xingru Guo Ziming Ma Yuangang Shi Yuankui Chu Xingang Dan |
| author_facet | Tianshu Dai Xiaolong Kang Chaoyun Yang Shan Mei Shihao Wei Xingru Guo Ziming Ma Yuangang Shi Yuankui Chu Xingang Dan |
| author_sort | Tianshu Dai |
| collection | DOAJ |
| description | Kisspeptin is a peptide hormone encoded by the kiss-1 gene that regulates animal reproduction. Our studies revealed that kisspeptin can regulate steroid hormone production and promote cell proliferation in ovarian granulosa cells of Tan sheep, but the mechanism has not yet been fully understood. We speculated that kisspeptin might promote steroid hormone production and cell proliferation by mediating the expression of specific miRNA and mRNA in granulosa cells. Accordingly, after granulosa cells were treated with kisspeptin, the RNA of cells was extracted to construct a cDNA library, and miRNA-mRNA sequencing was performed. Results showed that 1303 expressed genes and 605 expressed miRNAs were identified. Furthermore, eight differentially expressed miRNAs were found, and their target genes were significantly enriched in progesterone synthesis/metabolism, hormone biosynthesis, ovulation cycle, and steroid metabolism regulation. Meanwhile, mRNA was significantly enriched in steroid biosynthesis, IL-17 signaling pathway, and GnRH signaling pathway. Integrative analysis of miRNA-mRNA revealed that the significantly different oar-let-7b targets eight genes, of which EGR1 (early growth response-1) might play a significant role in regulating the function of granulosa cells, and miR-10a regulates lipid metabolism and steroid hormone synthesis by targeting HNRNPD. Additionally, PPI analysis revealed genes that are not miRNA targets but crucial to other biological processes in granulosa cells, implying that kisspeptin may also indirectly regulate granulosa cell function by these pathways. The findings of this work may help understand the molecular mechanism of kisspeptin regulating steroid hormone secretion, cell proliferation, and other physiological functions in ovarian granulosa cells of Tan sheep. |
| first_indexed | 2024-03-09T19:20:29Z |
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| id | doaj.art-d713998775974a08b52a74398edd5891 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T19:20:29Z |
| publishDate | 2022-10-01 |
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| series | Animals |
| spelling | doaj.art-d713998775974a08b52a74398edd58912023-11-24T03:25:09ZengMDPI AGAnimals2076-26152022-10-011221298910.3390/ani12212989Integrative Analysis of miRNA-mRNA in Ovarian Granulosa Cells Treated with Kisspeptin in Tan SheepTianshu Dai0Xiaolong Kang1Chaoyun Yang2Shan Mei3Shihao Wei4Xingru Guo5Ziming Ma6Yuangang Shi7Yuankui Chu8Xingang Dan9School of Agriculture, Ningxia University, Yinchuan 750021, ChinaSchool of Agriculture, Ningxia University, Yinchuan 750021, ChinaSchool of Agriculture, Ningxia University, Yinchuan 750021, ChinaAgriculture and Rural Bureau of Yeji District, Lu’an 237431, ChinaSchool of Agriculture, Ningxia University, Yinchuan 750021, ChinaSchool of Agriculture, Ningxia University, Yinchuan 750021, ChinaSchool of Agriculture, Ningxia University, Yinchuan 750021, ChinaSchool of Agriculture, Ningxia University, Yinchuan 750021, ChinaDepartment of Laboratory Medicine, General Hospital of Ningxia Medical University, Ningxia Medical University, Yinchuan 750004, ChinaSchool of Agriculture, Ningxia University, Yinchuan 750021, ChinaKisspeptin is a peptide hormone encoded by the kiss-1 gene that regulates animal reproduction. Our studies revealed that kisspeptin can regulate steroid hormone production and promote cell proliferation in ovarian granulosa cells of Tan sheep, but the mechanism has not yet been fully understood. We speculated that kisspeptin might promote steroid hormone production and cell proliferation by mediating the expression of specific miRNA and mRNA in granulosa cells. Accordingly, after granulosa cells were treated with kisspeptin, the RNA of cells was extracted to construct a cDNA library, and miRNA-mRNA sequencing was performed. Results showed that 1303 expressed genes and 605 expressed miRNAs were identified. Furthermore, eight differentially expressed miRNAs were found, and their target genes were significantly enriched in progesterone synthesis/metabolism, hormone biosynthesis, ovulation cycle, and steroid metabolism regulation. Meanwhile, mRNA was significantly enriched in steroid biosynthesis, IL-17 signaling pathway, and GnRH signaling pathway. Integrative analysis of miRNA-mRNA revealed that the significantly different oar-let-7b targets eight genes, of which EGR1 (early growth response-1) might play a significant role in regulating the function of granulosa cells, and miR-10a regulates lipid metabolism and steroid hormone synthesis by targeting HNRNPD. Additionally, PPI analysis revealed genes that are not miRNA targets but crucial to other biological processes in granulosa cells, implying that kisspeptin may also indirectly regulate granulosa cell function by these pathways. The findings of this work may help understand the molecular mechanism of kisspeptin regulating steroid hormone secretion, cell proliferation, and other physiological functions in ovarian granulosa cells of Tan sheep.https://www.mdpi.com/2076-2615/12/21/2989Tan sheepgranulosa cellsnext generationkisspeptin |
| spellingShingle | Tianshu Dai Xiaolong Kang Chaoyun Yang Shan Mei Shihao Wei Xingru Guo Ziming Ma Yuangang Shi Yuankui Chu Xingang Dan Integrative Analysis of miRNA-mRNA in Ovarian Granulosa Cells Treated with Kisspeptin in Tan Sheep Animals Tan sheep granulosa cells next generation kisspeptin |
| title | Integrative Analysis of miRNA-mRNA in Ovarian Granulosa Cells Treated with Kisspeptin in Tan Sheep |
| title_full | Integrative Analysis of miRNA-mRNA in Ovarian Granulosa Cells Treated with Kisspeptin in Tan Sheep |
| title_fullStr | Integrative Analysis of miRNA-mRNA in Ovarian Granulosa Cells Treated with Kisspeptin in Tan Sheep |
| title_full_unstemmed | Integrative Analysis of miRNA-mRNA in Ovarian Granulosa Cells Treated with Kisspeptin in Tan Sheep |
| title_short | Integrative Analysis of miRNA-mRNA in Ovarian Granulosa Cells Treated with Kisspeptin in Tan Sheep |
| title_sort | integrative analysis of mirna mrna in ovarian granulosa cells treated with kisspeptin in tan sheep |
| topic | Tan sheep granulosa cells next generation kisspeptin |
| url | https://www.mdpi.com/2076-2615/12/21/2989 |
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