Inhibition of AI-2 Quorum Sensing and Biofilm Formation in Campylobacter jejuni by Decanoic and Lauric Acids
Campylobacter jejuni is a major bacterial cause of human diarrheal diseases worldwide. Despite its sensitivity to environmental stresses, C. jejuni ubiquitously distributes throughout poultry production chains. Biofilm formation mediated by quorum sensing is suggested to be critical to the survival...
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.811506/full |
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| author | Shenmiao Li Shenmiao Li Kelvin Ka-wan Chan Marti Z. Hua Marti Z. Hua Greta Gölz Xiaonan Lu Xiaonan Lu |
| author_facet | Shenmiao Li Shenmiao Li Kelvin Ka-wan Chan Marti Z. Hua Marti Z. Hua Greta Gölz Xiaonan Lu Xiaonan Lu |
| author_sort | Shenmiao Li |
| collection | DOAJ |
| description | Campylobacter jejuni is a major bacterial cause of human diarrheal diseases worldwide. Despite its sensitivity to environmental stresses, C. jejuni ubiquitously distributes throughout poultry production chains. Biofilm formation mediated by quorum sensing is suggested to be critical to the survival of C. jejuni in agroecosystem. C. jejuni possesses LuxS, the enzyme involved in the production of autoinducer-2 (AI-2) signaling molecules. In this study, two fatty acids, namely decanoic acid and lauric acid, were identified to be effective in inhibiting AI-2 activity of C. jejuni. Both decanoic acid and lauric acid at 100 ppm inhibited ∼90% AI-2 activity (P < 0.05) of C. jejuni without bacterial inactivation. The biofilm biomass of two C. jejuni strains was reduced by 10–50% (P < 0.05) after treatment by both fatty acids, while increased biofilm formation was observed for one C. jejuni strain. In addition, both fatty acids effectively reduced the motility of all tested C. jejuni strains. These findings can aid in developing alternative C. jejuni control strategies in agri-food and clinical settings. |
| first_indexed | 2024-04-11T17:10:45Z |
| format | Article |
| id | doaj.art-d714da0680b74cd18c5c2f515a5e9780 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T17:10:45Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-d714da0680b74cd18c5c2f515a5e97802022-12-22T04:12:55ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-01-011210.3389/fmicb.2021.811506811506Inhibition of AI-2 Quorum Sensing and Biofilm Formation in Campylobacter jejuni by Decanoic and Lauric AcidsShenmiao Li0Shenmiao Li1Kelvin Ka-wan Chan2Marti Z. Hua3Marti Z. Hua4Greta Gölz5Xiaonan Lu6Xiaonan Lu7Food, Nutrition and Health Program, Faculty of Land and Food Systems, The University of British Columbia, Vancouver, BC, CanadaDepartment of Food Science and Agricultural Chemistry, Faculty of Agricultural and Environmental Sciences, McGill University, Montreal, QC, CanadaFood, Nutrition and Health Program, Faculty of Land and Food Systems, The University of British Columbia, Vancouver, BC, CanadaFood, Nutrition and Health Program, Faculty of Land and Food Systems, The University of British Columbia, Vancouver, BC, CanadaDepartment of Food Science and Agricultural Chemistry, Faculty of Agricultural and Environmental Sciences, McGill University, Montreal, QC, CanadaInstitute of Food Safety and Food Hygiene, Freie Universität Berlin, Berlin, GermanyFood, Nutrition and Health Program, Faculty of Land and Food Systems, The University of British Columbia, Vancouver, BC, CanadaDepartment of Food Science and Agricultural Chemistry, Faculty of Agricultural and Environmental Sciences, McGill University, Montreal, QC, CanadaCampylobacter jejuni is a major bacterial cause of human diarrheal diseases worldwide. Despite its sensitivity to environmental stresses, C. jejuni ubiquitously distributes throughout poultry production chains. Biofilm formation mediated by quorum sensing is suggested to be critical to the survival of C. jejuni in agroecosystem. C. jejuni possesses LuxS, the enzyme involved in the production of autoinducer-2 (AI-2) signaling molecules. In this study, two fatty acids, namely decanoic acid and lauric acid, were identified to be effective in inhibiting AI-2 activity of C. jejuni. Both decanoic acid and lauric acid at 100 ppm inhibited ∼90% AI-2 activity (P < 0.05) of C. jejuni without bacterial inactivation. The biofilm biomass of two C. jejuni strains was reduced by 10–50% (P < 0.05) after treatment by both fatty acids, while increased biofilm formation was observed for one C. jejuni strain. In addition, both fatty acids effectively reduced the motility of all tested C. jejuni strains. These findings can aid in developing alternative C. jejuni control strategies in agri-food and clinical settings.https://www.frontiersin.org/articles/10.3389/fmicb.2021.811506/fullCampylobacter jejuniquorum sensing (QS)biofilmmotilityfatty acids |
| spellingShingle | Shenmiao Li Shenmiao Li Kelvin Ka-wan Chan Marti Z. Hua Marti Z. Hua Greta Gölz Xiaonan Lu Xiaonan Lu Inhibition of AI-2 Quorum Sensing and Biofilm Formation in Campylobacter jejuni by Decanoic and Lauric Acids Frontiers in Microbiology Campylobacter jejuni quorum sensing (QS) biofilm motility fatty acids |
| title | Inhibition of AI-2 Quorum Sensing and Biofilm Formation in Campylobacter jejuni by Decanoic and Lauric Acids |
| title_full | Inhibition of AI-2 Quorum Sensing and Biofilm Formation in Campylobacter jejuni by Decanoic and Lauric Acids |
| title_fullStr | Inhibition of AI-2 Quorum Sensing and Biofilm Formation in Campylobacter jejuni by Decanoic and Lauric Acids |
| title_full_unstemmed | Inhibition of AI-2 Quorum Sensing and Biofilm Formation in Campylobacter jejuni by Decanoic and Lauric Acids |
| title_short | Inhibition of AI-2 Quorum Sensing and Biofilm Formation in Campylobacter jejuni by Decanoic and Lauric Acids |
| title_sort | inhibition of ai 2 quorum sensing and biofilm formation in campylobacter jejuni by decanoic and lauric acids |
| topic | Campylobacter jejuni quorum sensing (QS) biofilm motility fatty acids |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.811506/full |
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