Effect of Flagellin Pre-Exposure on the Inflammatory and Antifungal Response of Bronchial Epithelial Cells to Fungal Pathogens
Bronchial epithelial cells (BEC) play a crucial role in innate immunity against inhaled fungi. Indeed, in response to microorganisms, BEC synthesize proinflammatory cytokines involved in the recruitment of neutrophils. We have recently shown that BEC exert antifungal activity against <i>Asperg...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/12/1268 |
| _version_ | 1797456888579751936 |
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| author | Jeanne Bigot Manon Ruffin Juliette Guitard Sandra Vellaissamy Sophie Thorez Harriet Corvol Loïc Guillot Viviane Balloy Christophe Hennequin |
| author_facet | Jeanne Bigot Manon Ruffin Juliette Guitard Sandra Vellaissamy Sophie Thorez Harriet Corvol Loïc Guillot Viviane Balloy Christophe Hennequin |
| author_sort | Jeanne Bigot |
| collection | DOAJ |
| description | Bronchial epithelial cells (BEC) play a crucial role in innate immunity against inhaled fungi. Indeed, in response to microorganisms, BEC synthesize proinflammatory cytokines involved in the recruitment of neutrophils. We have recently shown that BEC exert antifungal activity against <i>Aspergillus fumigatus</i> by inhibiting filament growth. In the present study, we first analyzed the inflammatory and antifungal responses of BEC infected by several fungal species such as <i>Aspergillus</i> spp., <i>Scedosporium apiospermum</i> and <i>Candida albicans</i>, which are frequently isolated from the sputum of people with chronic pulmonary diseases. The airways of these patients, such as people with cystic fibrosis (pwCF), are mainly colonized by <i>P. aeruginosa</i> and secondary by fungal pathogens. We have previously demonstrated that BEC are capable of innate immune memory, allowing them to increase their inflammatory response against <i>A. fumigatus</i> following a previous contact with <i>Pseudomonas aeruginosa</i> flagellin. To identify the impact of bacteria exposure on BEC responses to other fungal infections, we extended the analysis of BEC innate immune memory to <i>Aspergillus</i> spp., <i>Scedosporium apiospermum</i> and <i>Candida albicans</i> infection. Our results show that BEC are able to recognize and respond to <i>Aspergillus</i> spp., <i>S. apiospermum</i> and <i>C. albicans</i> infection and that the modulation of BEC responses by pre-exposure to flagellin varies according to the fungal species encountered. Deepening our knowledge of the innate immune memory of BEC should open new therapeutic avenues to modulate the inflammatory response against polymicrobial infections observed in chronic pulmonary diseases such as CF. |
| first_indexed | 2024-03-09T16:14:18Z |
| format | Article |
| id | doaj.art-d719bfacc97b4d6aa269d6191fb0538b |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T16:14:18Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-d719bfacc97b4d6aa269d6191fb0538b2023-11-24T15:59:34ZengMDPI AGJournal of Fungi2309-608X2022-11-01812126810.3390/jof8121268Effect of Flagellin Pre-Exposure on the Inflammatory and Antifungal Response of Bronchial Epithelial Cells to Fungal PathogensJeanne Bigot0Manon Ruffin1Juliette Guitard2Sandra Vellaissamy3Sophie Thorez4Harriet Corvol5Loïc Guillot6Viviane Balloy7Christophe Hennequin8Centre de Recherche Saint-Antoine (CRSA), Inserm, AP-HP, Hôpital Saint-Antoine, Service de Parasitologie-Mycologie, Sorbonne Université, F-75012 Paris, FranceCentre de Recherche Saint-Antoine (CRSA), Inserm, Sorbonne Université, F-75012 Paris, FranceCentre de Recherche Saint-Antoine (CRSA), Inserm, AP-HP, Hôpital Saint-Antoine, Service de Parasitologie-Mycologie, Sorbonne Université, F-75012 Paris, FranceAP-HP, Hôpital Saint-Antoine, Service de Parasitologie-Mycologie, F-75012 Paris, FranceAP-HP, Hôpital Saint-Antoine, Service de Parasitologie-Mycologie, F-75012 Paris, FranceCentre de Recherche Saint-Antoine (CRSA), Inserm, AP-HP, Hôpital Trousseau, Service de Pneumologie Pédiatrique, Sorbonne Université, F-75012 Paris, FranceCentre de Recherche Saint-Antoine (CRSA), Inserm, Sorbonne Université, F-75012 Paris, FranceCentre de Recherche Saint-Antoine (CRSA), Inserm, Sorbonne Université, F-75012 Paris, FranceCentre de Recherche Saint-Antoine (CRSA), Inserm, AP-HP, Hôpital Saint-Antoine, Service de Parasitologie-Mycologie, Sorbonne Université, F-75012 Paris, FranceBronchial epithelial cells (BEC) play a crucial role in innate immunity against inhaled fungi. Indeed, in response to microorganisms, BEC synthesize proinflammatory cytokines involved in the recruitment of neutrophils. We have recently shown that BEC exert antifungal activity against <i>Aspergillus fumigatus</i> by inhibiting filament growth. In the present study, we first analyzed the inflammatory and antifungal responses of BEC infected by several fungal species such as <i>Aspergillus</i> spp., <i>Scedosporium apiospermum</i> and <i>Candida albicans</i>, which are frequently isolated from the sputum of people with chronic pulmonary diseases. The airways of these patients, such as people with cystic fibrosis (pwCF), are mainly colonized by <i>P. aeruginosa</i> and secondary by fungal pathogens. We have previously demonstrated that BEC are capable of innate immune memory, allowing them to increase their inflammatory response against <i>A. fumigatus</i> following a previous contact with <i>Pseudomonas aeruginosa</i> flagellin. To identify the impact of bacteria exposure on BEC responses to other fungal infections, we extended the analysis of BEC innate immune memory to <i>Aspergillus</i> spp., <i>Scedosporium apiospermum</i> and <i>Candida albicans</i> infection. Our results show that BEC are able to recognize and respond to <i>Aspergillus</i> spp., <i>S. apiospermum</i> and <i>C. albicans</i> infection and that the modulation of BEC responses by pre-exposure to flagellin varies according to the fungal species encountered. Deepening our knowledge of the innate immune memory of BEC should open new therapeutic avenues to modulate the inflammatory response against polymicrobial infections observed in chronic pulmonary diseases such as CF.https://www.mdpi.com/2309-608X/8/12/1268fungal infectionbronchial epithelial cellsinnate immune memorychronic pulmonary diseases |
| spellingShingle | Jeanne Bigot Manon Ruffin Juliette Guitard Sandra Vellaissamy Sophie Thorez Harriet Corvol Loïc Guillot Viviane Balloy Christophe Hennequin Effect of Flagellin Pre-Exposure on the Inflammatory and Antifungal Response of Bronchial Epithelial Cells to Fungal Pathogens Journal of Fungi fungal infection bronchial epithelial cells innate immune memory chronic pulmonary diseases |
| title | Effect of Flagellin Pre-Exposure on the Inflammatory and Antifungal Response of Bronchial Epithelial Cells to Fungal Pathogens |
| title_full | Effect of Flagellin Pre-Exposure on the Inflammatory and Antifungal Response of Bronchial Epithelial Cells to Fungal Pathogens |
| title_fullStr | Effect of Flagellin Pre-Exposure on the Inflammatory and Antifungal Response of Bronchial Epithelial Cells to Fungal Pathogens |
| title_full_unstemmed | Effect of Flagellin Pre-Exposure on the Inflammatory and Antifungal Response of Bronchial Epithelial Cells to Fungal Pathogens |
| title_short | Effect of Flagellin Pre-Exposure on the Inflammatory and Antifungal Response of Bronchial Epithelial Cells to Fungal Pathogens |
| title_sort | effect of flagellin pre exposure on the inflammatory and antifungal response of bronchial epithelial cells to fungal pathogens |
| topic | fungal infection bronchial epithelial cells innate immune memory chronic pulmonary diseases |
| url | https://www.mdpi.com/2309-608X/8/12/1268 |
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