Alpha tocopherol-Nanocellulose loaded alginate membranes and Pluronic hydrogels for diabetic wound healing
Due to the non-healing nature of chronic diabetic wounds, a novel wound healing agent is required to provide the nutrients to wound and cover the wound surfaces. In this study, Alginate (ALG) membrane and Pluronic® F-127(P) hydrogel incorporated with alpha-tocopherol (AT) and TEMPO-oxidized cellulos...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Materials & Design |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127522010279 |
| _version_ | 1797980314592608256 |
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| author | Anha Afrin Shefa Myeongki Park Jae-Gyoung Gwon Byong-Taek Lee |
| author_facet | Anha Afrin Shefa Myeongki Park Jae-Gyoung Gwon Byong-Taek Lee |
| author_sort | Anha Afrin Shefa |
| collection | DOAJ |
| description | Due to the non-healing nature of chronic diabetic wounds, a novel wound healing agent is required to provide the nutrients to wound and cover the wound surfaces. In this study, Alginate (ALG) membrane and Pluronic® F-127(P) hydrogel incorporated with alpha-tocopherol (AT) and TEMPO-oxidized cellulose nanofibers (TOCN) were designed. The ALG-TOCN-AT membrane was prepared using the casting method and P-TOCN-AT thermos-sensitive hydrogel was prepared using the cold mixing method. The gelation time of the P-TOCN-AT hydrogel was 45 ± 1.2 s at 37 °C and the viscosity of the hydrogel at 37 °C was higher than the viscosity measured at 25 °C. Further, the addition of TOCN and AT significantly improved the tensile strain of the membrane. An immediate release of AT was achieved in P-TOCN-AT gel while the ALG-TOCN-AT membrane released AT in a sustained manner. Cell proliferation study revealed the nontoxic nature of the hydrogel and membrane. Full-thickness skin wounds of diabetic rats were better healed by combined use of membrane and hydrogel compared to individual application of membrane or hydrogel. The highest neoepithelialization and angiogenesis was confirmed through CD31, CD105, and CD34 gene expression analysis. Hence, the combined application of hydrogels and membranes showed enhanced angiogenesis along with accelerated wound healing with no observable in-vivo toxicity. |
| first_indexed | 2024-04-11T05:53:39Z |
| format | Article |
| id | doaj.art-d719c6b686a149dfb02f187744f372c7 |
| institution | Directory Open Access Journal |
| issn | 0264-1275 |
| language | English |
| last_indexed | 2024-04-11T05:53:39Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj.art-d719c6b686a149dfb02f187744f372c72022-12-22T04:42:00ZengElsevierMaterials & Design0264-12752022-12-01224111404Alpha tocopherol-Nanocellulose loaded alginate membranes and Pluronic hydrogels for diabetic wound healingAnha Afrin Shefa0Myeongki Park1Jae-Gyoung Gwon2Byong-Taek Lee3Department of Regenerative Medicine, College of Medicine, Soonchunhyang University, Cheonan, Republic of KoreaDepartment of Regenerative Medicine, College of Medicine, Soonchunhyang University, Cheonan, Republic of KoreaDivision of Environmental Material Engineering, Department of Forest Products, National Institute of Forest Science, Seoul, Republic of KoreaDepartment of Regenerative Medicine, College of Medicine, Soonchunhyang University, Cheonan, Republic of Korea; Institute of Tissue Regeneration, Soonchunhyang University, Cheonan, Republic of Korea; Corresponding author at: Department of Regenerative Medicine, College of Medicine, Soonchunhyang University, Cheonan, Republic of Korea.Due to the non-healing nature of chronic diabetic wounds, a novel wound healing agent is required to provide the nutrients to wound and cover the wound surfaces. In this study, Alginate (ALG) membrane and Pluronic® F-127(P) hydrogel incorporated with alpha-tocopherol (AT) and TEMPO-oxidized cellulose nanofibers (TOCN) were designed. The ALG-TOCN-AT membrane was prepared using the casting method and P-TOCN-AT thermos-sensitive hydrogel was prepared using the cold mixing method. The gelation time of the P-TOCN-AT hydrogel was 45 ± 1.2 s at 37 °C and the viscosity of the hydrogel at 37 °C was higher than the viscosity measured at 25 °C. Further, the addition of TOCN and AT significantly improved the tensile strain of the membrane. An immediate release of AT was achieved in P-TOCN-AT gel while the ALG-TOCN-AT membrane released AT in a sustained manner. Cell proliferation study revealed the nontoxic nature of the hydrogel and membrane. Full-thickness skin wounds of diabetic rats were better healed by combined use of membrane and hydrogel compared to individual application of membrane or hydrogel. The highest neoepithelialization and angiogenesis was confirmed through CD31, CD105, and CD34 gene expression analysis. Hence, the combined application of hydrogels and membranes showed enhanced angiogenesis along with accelerated wound healing with no observable in-vivo toxicity.http://www.sciencedirect.com/science/article/pii/S0264127522010279Diabetic Wound HealingAlpha-TocopherolTEMPO-oxidized Cellulose NanofibersAlginate based MembranePluronic based Hydrogel |
| spellingShingle | Anha Afrin Shefa Myeongki Park Jae-Gyoung Gwon Byong-Taek Lee Alpha tocopherol-Nanocellulose loaded alginate membranes and Pluronic hydrogels for diabetic wound healing Materials & Design Diabetic Wound Healing Alpha-Tocopherol TEMPO-oxidized Cellulose Nanofibers Alginate based Membrane Pluronic based Hydrogel |
| title | Alpha tocopherol-Nanocellulose loaded alginate membranes and Pluronic hydrogels for diabetic wound healing |
| title_full | Alpha tocopherol-Nanocellulose loaded alginate membranes and Pluronic hydrogels for diabetic wound healing |
| title_fullStr | Alpha tocopherol-Nanocellulose loaded alginate membranes and Pluronic hydrogels for diabetic wound healing |
| title_full_unstemmed | Alpha tocopherol-Nanocellulose loaded alginate membranes and Pluronic hydrogels for diabetic wound healing |
| title_short | Alpha tocopherol-Nanocellulose loaded alginate membranes and Pluronic hydrogels for diabetic wound healing |
| title_sort | alpha tocopherol nanocellulose loaded alginate membranes and pluronic hydrogels for diabetic wound healing |
| topic | Diabetic Wound Healing Alpha-Tocopherol TEMPO-oxidized Cellulose Nanofibers Alginate based Membrane Pluronic based Hydrogel |
| url | http://www.sciencedirect.com/science/article/pii/S0264127522010279 |
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