Fabrication and Characterization of Apple-Pectin–PVA-Based Nanofibers for Improved Viability of Probiotics
In the current study, apple-pectin-based novel nanofibers were fabricated by electrospinning. Polyvinyl alcohol (PVA) and apple pectin (PEC) solution were mixed to obtain an optimized ratio for the preparation of electrospun nanofibers. The obtained nanofibers were characterized for their physiochem...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/17/3194 |
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| author | Asad Nawaz Sana Irshad Noman Walayat Mohammad Rizwan Khan Muhammad Waheed Iqbal Xiaofang Luo |
| author_facet | Asad Nawaz Sana Irshad Noman Walayat Mohammad Rizwan Khan Muhammad Waheed Iqbal Xiaofang Luo |
| author_sort | Asad Nawaz |
| collection | DOAJ |
| description | In the current study, apple-pectin-based novel nanofibers were fabricated by electrospinning. Polyvinyl alcohol (PVA) and apple pectin (PEC) solution were mixed to obtain an optimized ratio for the preparation of electrospun nanofibers. The obtained nanofibers were characterized for their physiochemical, mechanical and thermal properties. The nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy and thermogravimetric analysis (TGA). Furthermore, an assay of the in vitro viability of free and encapsulated probiotics was carried out under simulated gastrointestinal conditions. The results of TGA revealed that the PVA/PEC nanofibers had good thermal stability. The probiotics encapsulated by electrospinning showed a high survival rate as compared to free cells under simulated gastrointestinal conditions. Furthermore, encapsulated probiotics and free cells showed a 3 log (cfu/mL) and 10 log (cfu/mL) reduction, respectively, from 30 to 120 min of simulated digestion. These findings indicate that the PVA/PEC-based nanofibers have good barrier properties and could potentially be used for the improved viability of probiotics under simulated gastrointestinal conditions and in the development of functional foods. |
| first_indexed | 2024-03-10T23:24:05Z |
| format | Article |
| id | doaj.art-d71b6dae2e5946dfb4afb1998bcfc67c |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T23:24:05Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-d71b6dae2e5946dfb4afb1998bcfc67c2023-11-19T08:08:11ZengMDPI AGFoods2304-81582023-08-011217319410.3390/foods12173194Fabrication and Characterization of Apple-Pectin–PVA-Based Nanofibers for Improved Viability of ProbioticsAsad Nawaz0Sana Irshad1Noman Walayat2Mohammad Rizwan Khan3Muhammad Waheed Iqbal4Xiaofang Luo5Hunan Engineering Technology Research Center for Comprehensive Development and Utilization of Biomass Resources, College of Chemistry and Bioengineering, Hunan University of Science and Engineering, Yangzhou 425199, ChinaInstitute for Advanced Study, Shenzhen University, Shenzhen 518060, ChinaCollege of Tea Science and Tea Culture, Zhejiang Agriculture and Forestry University, Hangzhou 310007, ChinaDepartment of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaHunan Engineering Technology Research Center for Comprehensive Development and Utilization of Biomass Resources, College of Chemistry and Bioengineering, Hunan University of Science and Engineering, Yangzhou 425199, ChinaIn the current study, apple-pectin-based novel nanofibers were fabricated by electrospinning. Polyvinyl alcohol (PVA) and apple pectin (PEC) solution were mixed to obtain an optimized ratio for the preparation of electrospun nanofibers. The obtained nanofibers were characterized for their physiochemical, mechanical and thermal properties. The nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy and thermogravimetric analysis (TGA). Furthermore, an assay of the in vitro viability of free and encapsulated probiotics was carried out under simulated gastrointestinal conditions. The results of TGA revealed that the PVA/PEC nanofibers had good thermal stability. The probiotics encapsulated by electrospinning showed a high survival rate as compared to free cells under simulated gastrointestinal conditions. Furthermore, encapsulated probiotics and free cells showed a 3 log (cfu/mL) and 10 log (cfu/mL) reduction, respectively, from 30 to 120 min of simulated digestion. These findings indicate that the PVA/PEC-based nanofibers have good barrier properties and could potentially be used for the improved viability of probiotics under simulated gastrointestinal conditions and in the development of functional foods.https://www.mdpi.com/2304-8158/12/17/3194apple pectinnanofibersprobioticselectrospinningviability |
| spellingShingle | Asad Nawaz Sana Irshad Noman Walayat Mohammad Rizwan Khan Muhammad Waheed Iqbal Xiaofang Luo Fabrication and Characterization of Apple-Pectin–PVA-Based Nanofibers for Improved Viability of Probiotics Foods apple pectin nanofibers probiotics electrospinning viability |
| title | Fabrication and Characterization of Apple-Pectin–PVA-Based Nanofibers for Improved Viability of Probiotics |
| title_full | Fabrication and Characterization of Apple-Pectin–PVA-Based Nanofibers for Improved Viability of Probiotics |
| title_fullStr | Fabrication and Characterization of Apple-Pectin–PVA-Based Nanofibers for Improved Viability of Probiotics |
| title_full_unstemmed | Fabrication and Characterization of Apple-Pectin–PVA-Based Nanofibers for Improved Viability of Probiotics |
| title_short | Fabrication and Characterization of Apple-Pectin–PVA-Based Nanofibers for Improved Viability of Probiotics |
| title_sort | fabrication and characterization of apple pectin pva based nanofibers for improved viability of probiotics |
| topic | apple pectin nanofibers probiotics electrospinning viability |
| url | https://www.mdpi.com/2304-8158/12/17/3194 |
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