Cytokine response to the RSV antigen delivered by dendritic cell-directed vaccination in congenic chicken lines
Abstract Systems of antigen delivery into antigen-presenting cells represent an important novel strategy in chicken vaccine development. In this study, we verified the ability of Rous sarcoma virus (RSV) antigens fused with streptavidin to be targeted by specific biotinylated monoclonal antibody (an...
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| Format: | Article |
| Language: | English |
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BMC
2017-04-01
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| Series: | Veterinary Research |
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| Online Access: | http://link.springer.com/article/10.1186/s13567-017-0423-8 |
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| author | Jitka Mucksová Jiří Plachý Ondřej Staněk Jiří Hejnar Jiří Kalina Barbora Benešová Pavel Trefil |
| author_facet | Jitka Mucksová Jiří Plachý Ondřej Staněk Jiří Hejnar Jiří Kalina Barbora Benešová Pavel Trefil |
| author_sort | Jitka Mucksová |
| collection | DOAJ |
| description | Abstract Systems of antigen delivery into antigen-presenting cells represent an important novel strategy in chicken vaccine development. In this study, we verified the ability of Rous sarcoma virus (RSV) antigens fused with streptavidin to be targeted by specific biotinylated monoclonal antibody (anti-CD205) into dendritic cells and induce virus-specific protective immunity. The method was tested in four congenic lines of chickens that are either resistant or susceptible to the progressive growth of RSV-induced tumors. Our analyses confirmed that the biot-anti-CD205-SA-FITC complex was internalized by chicken splenocytes. In the cytokine expression profile, several significant differences were evident between RSV-challenged progressor and regressor chicken lines. A significant up-regulation of IL-2, IL-12, IL-15, and IL-18 expression was detected in immunized chickens of both regressor and progressor groups. Of these cytokines, IL-2 and IL-12 were most up-regulated 14 days post-challenge (dpc), while IL-15 and IL-18 were most up-regulated at 28 dpc. On the contrary, IL-10 expression was significantly down-regulated in all immunized groups of progressor chickens at 14 dpc. We detected significant up-regulation of IL-17 in the group of immunized progressors. LITAF down-regulation with iNOS up-regulation was especially observed in the progressor group of immunized chickens that developed large tumors. Based on the increased expression of cytokines specific for activated dendritic cells, we conclude that our system is able to induce partial stimulation of specific cell types involved in cell-mediated immunity. |
| first_indexed | 2024-12-19T22:20:08Z |
| format | Article |
| id | doaj.art-d71cef635c5043d6975ecccc7196d46e |
| institution | Directory Open Access Journal |
| issn | 1297-9716 |
| language | English |
| last_indexed | 2024-12-19T22:20:08Z |
| publishDate | 2017-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-d71cef635c5043d6975ecccc7196d46e2022-12-21T20:03:39ZengBMCVeterinary Research1297-97162017-04-0148111410.1186/s13567-017-0423-8Cytokine response to the RSV antigen delivered by dendritic cell-directed vaccination in congenic chicken linesJitka Mucksová0Jiří Plachý1Ondřej Staněk2Jiří Hejnar3Jiří Kalina4Barbora Benešová5Pavel Trefil6BIOPHARM, Research Institute of Biopharmacy and Veterinary DrugsInstitute of Molecular Genetics, Academy of Sciences of the Czech RepublicInstitute of Microbiology, Academy of Sciences of the Czech RepublicInstitute of Molecular Genetics, Academy of Sciences of the Czech RepublicBIOPHARM, Research Institute of Biopharmacy and Veterinary DrugsBIOPHARM, Research Institute of Biopharmacy and Veterinary DrugsBIOPHARM, Research Institute of Biopharmacy and Veterinary DrugsAbstract Systems of antigen delivery into antigen-presenting cells represent an important novel strategy in chicken vaccine development. In this study, we verified the ability of Rous sarcoma virus (RSV) antigens fused with streptavidin to be targeted by specific biotinylated monoclonal antibody (anti-CD205) into dendritic cells and induce virus-specific protective immunity. The method was tested in four congenic lines of chickens that are either resistant or susceptible to the progressive growth of RSV-induced tumors. Our analyses confirmed that the biot-anti-CD205-SA-FITC complex was internalized by chicken splenocytes. In the cytokine expression profile, several significant differences were evident between RSV-challenged progressor and regressor chicken lines. A significant up-regulation of IL-2, IL-12, IL-15, and IL-18 expression was detected in immunized chickens of both regressor and progressor groups. Of these cytokines, IL-2 and IL-12 were most up-regulated 14 days post-challenge (dpc), while IL-15 and IL-18 were most up-regulated at 28 dpc. On the contrary, IL-10 expression was significantly down-regulated in all immunized groups of progressor chickens at 14 dpc. We detected significant up-regulation of IL-17 in the group of immunized progressors. LITAF down-regulation with iNOS up-regulation was especially observed in the progressor group of immunized chickens that developed large tumors. Based on the increased expression of cytokines specific for activated dendritic cells, we conclude that our system is able to induce partial stimulation of specific cell types involved in cell-mediated immunity.http://link.springer.com/article/10.1186/s13567-017-0423-8Natural Killer CellMean Fluorescence IntensityInfectious Bursal Disease VirusRous Sarcoma VirusAntigen Delivery |
| spellingShingle | Jitka Mucksová Jiří Plachý Ondřej Staněk Jiří Hejnar Jiří Kalina Barbora Benešová Pavel Trefil Cytokine response to the RSV antigen delivered by dendritic cell-directed vaccination in congenic chicken lines Veterinary Research Natural Killer Cell Mean Fluorescence Intensity Infectious Bursal Disease Virus Rous Sarcoma Virus Antigen Delivery |
| title | Cytokine response to the RSV antigen delivered by dendritic cell-directed vaccination in congenic chicken lines |
| title_full | Cytokine response to the RSV antigen delivered by dendritic cell-directed vaccination in congenic chicken lines |
| title_fullStr | Cytokine response to the RSV antigen delivered by dendritic cell-directed vaccination in congenic chicken lines |
| title_full_unstemmed | Cytokine response to the RSV antigen delivered by dendritic cell-directed vaccination in congenic chicken lines |
| title_short | Cytokine response to the RSV antigen delivered by dendritic cell-directed vaccination in congenic chicken lines |
| title_sort | cytokine response to the rsv antigen delivered by dendritic cell directed vaccination in congenic chicken lines |
| topic | Natural Killer Cell Mean Fluorescence Intensity Infectious Bursal Disease Virus Rous Sarcoma Virus Antigen Delivery |
| url | http://link.springer.com/article/10.1186/s13567-017-0423-8 |
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