The Identification of Small RNAs Differentially Expressed in Apple Buds Reveals a Potential Role of the Mir159-MYB Regulatory Module during Dormancy
Winter dormancy is an adaptative mechanism that temperate and boreal trees have developed to protect their meristems against low temperatures. In apple trees (<i>Malus domestica</i>), cold temperatures induce bud dormancy at the end of summer/beginning of the fall. Apple buds stay dorman...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/12/2665 |
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| author | Julio Garighan Etienne Dvorak Joan Estevan Karine Loridon Bruno Huettel Gautier Sarah Isabelle Farrera Julie Leclercq Priscila Grynberg Roberto Coiti Togawa Marcos Mota do Carmo Costa Evelyne Costes Fernando Andrés |
| author_facet | Julio Garighan Etienne Dvorak Joan Estevan Karine Loridon Bruno Huettel Gautier Sarah Isabelle Farrera Julie Leclercq Priscila Grynberg Roberto Coiti Togawa Marcos Mota do Carmo Costa Evelyne Costes Fernando Andrés |
| author_sort | Julio Garighan |
| collection | DOAJ |
| description | Winter dormancy is an adaptative mechanism that temperate and boreal trees have developed to protect their meristems against low temperatures. In apple trees (<i>Malus domestica</i>), cold temperatures induce bud dormancy at the end of summer/beginning of the fall. Apple buds stay dormant during winter until they are exposed to a period of cold, after which they can resume growth (budbreak) and initiate flowering in response to warmer temperatures in spring. It is well-known that small RNAs modulate temperature responses in many plant species, but however, how small RNAs are involved in genetic networks of temperature-mediated dormancy control in fruit tree species remains unclear. Here, we have made use of a recently developed ARGONAUTE (AGO)-purification technique to isolate small RNAs from apple buds. A small RNA-seq experiment resulted in the identification of 17 micro RNAs (miRNAs) that change their pattern of expression in apple buds during dormancy. Furthermore, the functional analysis of their predicted target genes suggests a main role of the 17 miRNAs in phenylpropanoid biosynthesis, gene regulation, plant development and growth, and response to stimulus. Finally, we studied the conservation of the <i>Arabidopsis thaliana</i> regulatory miR159-MYB module in apple in the context of the plant hormone abscisic acid homeostasis. |
| first_indexed | 2024-03-10T03:17:53Z |
| format | Article |
| id | doaj.art-d71d7e8cc15a44f89956f77778cd4b59 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T03:17:53Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-d71d7e8cc15a44f89956f77778cd4b592023-11-23T10:11:25ZengMDPI AGPlants2223-77472021-12-011012266510.3390/plants10122665The Identification of Small RNAs Differentially Expressed in Apple Buds Reveals a Potential Role of the Mir159-MYB Regulatory Module during DormancyJulio Garighan0Etienne Dvorak1Joan Estevan2Karine Loridon3Bruno Huettel4Gautier Sarah5Isabelle Farrera6Julie Leclercq7Priscila Grynberg8Roberto Coiti Togawa9Marcos Mota do Carmo Costa10Evelyne Costes11Fernando Andrés12UMR AGAP Institute, Institut Agro, CIRAD, INRAE, University of Montpellier, F-34398 Montpellier, FranceUMR AGAP Institute, Institut Agro, CIRAD, INRAE, University of Montpellier, F-34398 Montpellier, FranceUMR AGAP Institute, Institut Agro, CIRAD, INRAE, University of Montpellier, F-34398 Montpellier, FranceUMR AGAP Institute, Institut Agro, CIRAD, INRAE, University of Montpellier, F-34398 Montpellier, FranceGenome Centre, Max Planck Institute for Plant Breeding Research, D-50829 Cologne, GermanyUMR AGAP Institute, Institut Agro, CIRAD, INRAE, University of Montpellier, F-34398 Montpellier, FranceUMR AGAP Institute, Institut Agro, CIRAD, INRAE, University of Montpellier, F-34398 Montpellier, FranceUMR AGAP Institute, Institut Agro, CIRAD, INRAE, University of Montpellier, F-34398 Montpellier, FranceBioinformatica Laboratory, Embrapa Recursos Genéticos e Biotecnologia—Cenargen, Brasilia 02372, BrazilBioinformatica Laboratory, Embrapa Recursos Genéticos e Biotecnologia—Cenargen, Brasilia 02372, BrazilBioinformatica Laboratory, Embrapa Recursos Genéticos e Biotecnologia—Cenargen, Brasilia 02372, BrazilUMR AGAP Institute, Institut Agro, CIRAD, INRAE, University of Montpellier, F-34398 Montpellier, FranceUMR AGAP Institute, Institut Agro, CIRAD, INRAE, University of Montpellier, F-34398 Montpellier, FranceWinter dormancy is an adaptative mechanism that temperate and boreal trees have developed to protect their meristems against low temperatures. In apple trees (<i>Malus domestica</i>), cold temperatures induce bud dormancy at the end of summer/beginning of the fall. Apple buds stay dormant during winter until they are exposed to a period of cold, after which they can resume growth (budbreak) and initiate flowering in response to warmer temperatures in spring. It is well-known that small RNAs modulate temperature responses in many plant species, but however, how small RNAs are involved in genetic networks of temperature-mediated dormancy control in fruit tree species remains unclear. Here, we have made use of a recently developed ARGONAUTE (AGO)-purification technique to isolate small RNAs from apple buds. A small RNA-seq experiment resulted in the identification of 17 micro RNAs (miRNAs) that change their pattern of expression in apple buds during dormancy. Furthermore, the functional analysis of their predicted target genes suggests a main role of the 17 miRNAs in phenylpropanoid biosynthesis, gene regulation, plant development and growth, and response to stimulus. Finally, we studied the conservation of the <i>Arabidopsis thaliana</i> regulatory miR159-MYB module in apple in the context of the plant hormone abscisic acid homeostasis.https://www.mdpi.com/2223-7747/10/12/2665dormancyapple treesmall RNAsmiR159 |
| spellingShingle | Julio Garighan Etienne Dvorak Joan Estevan Karine Loridon Bruno Huettel Gautier Sarah Isabelle Farrera Julie Leclercq Priscila Grynberg Roberto Coiti Togawa Marcos Mota do Carmo Costa Evelyne Costes Fernando Andrés The Identification of Small RNAs Differentially Expressed in Apple Buds Reveals a Potential Role of the Mir159-MYB Regulatory Module during Dormancy Plants dormancy apple tree small RNAs miR159 |
| title | The Identification of Small RNAs Differentially Expressed in Apple Buds Reveals a Potential Role of the Mir159-MYB Regulatory Module during Dormancy |
| title_full | The Identification of Small RNAs Differentially Expressed in Apple Buds Reveals a Potential Role of the Mir159-MYB Regulatory Module during Dormancy |
| title_fullStr | The Identification of Small RNAs Differentially Expressed in Apple Buds Reveals a Potential Role of the Mir159-MYB Regulatory Module during Dormancy |
| title_full_unstemmed | The Identification of Small RNAs Differentially Expressed in Apple Buds Reveals a Potential Role of the Mir159-MYB Regulatory Module during Dormancy |
| title_short | The Identification of Small RNAs Differentially Expressed in Apple Buds Reveals a Potential Role of the Mir159-MYB Regulatory Module during Dormancy |
| title_sort | identification of small rnas differentially expressed in apple buds reveals a potential role of the mir159 myb regulatory module during dormancy |
| topic | dormancy apple tree small RNAs miR159 |
| url | https://www.mdpi.com/2223-7747/10/12/2665 |
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