In vitro multiplication of Tabernaemontana fuchsiaefolia L. (Apocynaceae)
This study describes a simple and promising for in vitro multiplication of Tabernaemontana fuchsiaefolia, a species abundantly found in southern Brazil utilized for medicinal purposes and as a source of compounds that may be used to develop new synthetic drugs. Apical and hypocotyl explants were cul...
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| Format: | Article |
| Language: | English |
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Sociedade de Investigações Florestais
2003-08-01
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| Series: | Revista Árvore |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622003000400001&tlng=en |
| _version_ | 1818744892792242176 |
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| author | Arildo José Braz de Oliveira Vanda Marilza de Carvalho Alexandre Ferreira Fernando Y. Sato Maria de Fátima Pires da Silva Machado |
| author_facet | Arildo José Braz de Oliveira Vanda Marilza de Carvalho Alexandre Ferreira Fernando Y. Sato Maria de Fátima Pires da Silva Machado |
| author_sort | Arildo José Braz de Oliveira |
| collection | DOAJ |
| description | This study describes a simple and promising for in vitro multiplication of Tabernaemontana fuchsiaefolia, a species abundantly found in southern Brazil utilized for medicinal purposes and as a source of compounds that may be used to develop new synthetic drugs. Apical and hypocotyl explants were cultured in MS medium containing different concentrations of the cytokinins benzylaminopurine (BA) and 6-furfurylaminopurine (kinetin), supplemented with phloroglucinol (1, 3, 5-hydroxybenzene) to stimulate growth and shoot proliferation. Cytokinin added to the culture media positively influenced the micropropagation of T. fuchsiaefolia.and kinetin induced more shoots per explant than BA cytokinin. A favorable effect of phloroglucinol on apical and lateral buds from hypocotyls was also achieved in medium containing no kinetin or in all kinetin concentrations tested. Short pulses of auxin 3-indolebutyric acid (IBA) 5.0 mg/l resulted in satisfactory rooting in apical microcuttings. The addition of phloroglucinol to MS medium induced rhizogenesis in 29% of the nodal segments transferred to MS medium in the absence of IBA and in 50% of the nodal segments transferred to MS medium containing 0.5 mg/l IBA and in nodal segments previously submitted to short pulses of IBA. |
| first_indexed | 2024-12-18T02:51:32Z |
| format | Article |
| id | doaj.art-d71dc2a3788a43249aef73c099ba8a5d |
| institution | Directory Open Access Journal |
| issn | 1806-9088 |
| language | English |
| last_indexed | 2024-12-18T02:51:32Z |
| publishDate | 2003-08-01 |
| publisher | Sociedade de Investigações Florestais |
| record_format | Article |
| series | Revista Árvore |
| spelling | doaj.art-d71dc2a3788a43249aef73c099ba8a5d2022-12-21T21:23:27ZengSociedade de Investigações FlorestaisRevista Árvore1806-90882003-08-0127442142510.1590/S0100-67622003000400001In vitro multiplication of Tabernaemontana fuchsiaefolia L. (Apocynaceae)Arildo José Braz de Oliveira0Vanda Marilza de Carvalho1Alexandre Ferreira2Fernando Y. Sato3Maria de Fátima Pires da Silva Machado4Universidade Estadual de MaringáUniversidade Estadual de MaringáUniversidade Estadual de MaringáUniversidade Estadual de MaringáUniversidade Estadual de MaringáThis study describes a simple and promising for in vitro multiplication of Tabernaemontana fuchsiaefolia, a species abundantly found in southern Brazil utilized for medicinal purposes and as a source of compounds that may be used to develop new synthetic drugs. Apical and hypocotyl explants were cultured in MS medium containing different concentrations of the cytokinins benzylaminopurine (BA) and 6-furfurylaminopurine (kinetin), supplemented with phloroglucinol (1, 3, 5-hydroxybenzene) to stimulate growth and shoot proliferation. Cytokinin added to the culture media positively influenced the micropropagation of T. fuchsiaefolia.and kinetin induced more shoots per explant than BA cytokinin. A favorable effect of phloroglucinol on apical and lateral buds from hypocotyls was also achieved in medium containing no kinetin or in all kinetin concentrations tested. Short pulses of auxin 3-indolebutyric acid (IBA) 5.0 mg/l resulted in satisfactory rooting in apical microcuttings. The addition of phloroglucinol to MS medium induced rhizogenesis in 29% of the nodal segments transferred to MS medium in the absence of IBA and in 50% of the nodal segments transferred to MS medium containing 0.5 mg/l IBA and in nodal segments previously submitted to short pulses of IBA.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622003000400001&tlng=enmicropropagationTabernaemontana fuchsiaefoliaApocynaceaetissue culture |
| spellingShingle | Arildo José Braz de Oliveira Vanda Marilza de Carvalho Alexandre Ferreira Fernando Y. Sato Maria de Fátima Pires da Silva Machado In vitro multiplication of Tabernaemontana fuchsiaefolia L. (Apocynaceae) Revista Árvore micropropagation Tabernaemontana fuchsiaefolia Apocynaceae tissue culture |
| title | In vitro multiplication of Tabernaemontana fuchsiaefolia L. (Apocynaceae) |
| title_full | In vitro multiplication of Tabernaemontana fuchsiaefolia L. (Apocynaceae) |
| title_fullStr | In vitro multiplication of Tabernaemontana fuchsiaefolia L. (Apocynaceae) |
| title_full_unstemmed | In vitro multiplication of Tabernaemontana fuchsiaefolia L. (Apocynaceae) |
| title_short | In vitro multiplication of Tabernaemontana fuchsiaefolia L. (Apocynaceae) |
| title_sort | in vitro multiplication of tabernaemontana fuchsiaefolia l apocynaceae |
| topic | micropropagation Tabernaemontana fuchsiaefolia Apocynaceae tissue culture |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-67622003000400001&tlng=en |
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