Cloning, nucleotide characterization and modeling expression of phytase gene phyC from Bacillus subtilis
Phytase has a hydrolysis function on phytic acid which yields inorganic phosphate. Using phytase enzymes in domestic animals have a positive effect on optimal diet intake. Bacillus species can only produce thermostable alkaline phytase. Accordingly extracellular phytase gene was isolated from <em...
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| Format: | Article |
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Shahid Bahonar University of Kerman
2012-08-01
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| Series: | مجله بیوتکنولوژی کشاورزی |
| Subjects: | |
| Online Access: | https://jab.uk.ac.ir/article_465_e5aa57d86a4dd8f90dbc33c8faf15c92.pdf |
| _version_ | 1818527102292459520 |
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| author | Hamid Arian nejad Mohammadreza Nassiri Aliasghar Aslaminejad Mojtaba Thmoors poor Reza valizadeh Ahmad Asoodeh SHahrokh GHovati |
| author_facet | Hamid Arian nejad Mohammadreza Nassiri Aliasghar Aslaminejad Mojtaba Thmoors poor Reza valizadeh Ahmad Asoodeh SHahrokh GHovati |
| author_sort | Hamid Arian nejad |
| collection | DOAJ |
| description | Phytase has a hydrolysis function on phytic acid which yields inorganic phosphate. Using phytase enzymes in domestic animals have a positive effect on optimal diet intake. Bacillus species can only produce thermostable alkaline phytase. Accordingly extracellular phytase gene was isolated from <em>Bacillus subtilis</em> ATCC 12711 (<em>phyc</em>) using linker primers containing restriction sites of <em>Bam</em>HI and <em>Hind</em>III. The isolated fragment was then inserted into pTZ57R/T vector for cloning and nucleotide characterization. Molecular weight of phytase protein was estimated about 42 kDa. On the other hand the model of phytase secondary structure showed the potential of thermostable characterization of enzyme |
| first_indexed | 2024-12-11T06:31:45Z |
| format | Article |
| id | doaj.art-d71ead928c5e4c0ea2870a0c808baedf |
| institution | Directory Open Access Journal |
| issn | 2228-6705 2228-6500 |
| language | fas |
| last_indexed | 2024-12-11T06:31:45Z |
| publishDate | 2012-08-01 |
| publisher | Shahid Bahonar University of Kerman |
| record_format | Article |
| series | مجله بیوتکنولوژی کشاورزی |
| spelling | doaj.art-d71ead928c5e4c0ea2870a0c808baedf2022-12-22T01:17:30ZfasShahid Bahonar University of Kermanمجله بیوتکنولوژی کشاورزی2228-67052228-65002012-08-0141193310.22103/jab.2012.465465Cloning, nucleotide characterization and modeling expression of phytase gene phyC from Bacillus subtilisHamid Arian nejad0Mohammadreza Nassiri1Aliasghar Aslaminejad2Mojtaba Thmoors poor3Reza valizadeh4Ahmad Asoodeh5SHahrokh GHovati61 دانشجوی کارشناسی ارشد، گروه علوم دامی، دانشگاه فردوسی مشهد2دانشیار، گروه علوم دامی، دانشکده کشاورزی، دانشگاه فردوسی مشهد 3دانشیار، گروه پژوهشی بیوتکنولوژی کشاورزی و دامی، پژوهشکده فناوری زیستی، دانشگاه فردوسی مشهاستادیار، گروه علوم دامی، دانشکده کشاورزی، دانشگاه فردوسی مشهددانشیار، گروه علوم دامی، دانشکده کشاورزی، دانشگاه فردوسی مشهداستاد، گروه علوم دامی، دانشکده کشاورزی، دانشگاه فردوسی مشهداستادیار، گروه بیوشیمی، دانشکده علوم، دانشگاه فردوسی مشهددانشجوی دکتری، گروه علوم دامی، دانشکده کشاورزی، دانشگاه فردوسی مشهدPhytase has a hydrolysis function on phytic acid which yields inorganic phosphate. Using phytase enzymes in domestic animals have a positive effect on optimal diet intake. Bacillus species can only produce thermostable alkaline phytase. Accordingly extracellular phytase gene was isolated from <em>Bacillus subtilis</em> ATCC 12711 (<em>phyc</em>) using linker primers containing restriction sites of <em>Bam</em>HI and <em>Hind</em>III. The isolated fragment was then inserted into pTZ57R/T vector for cloning and nucleotide characterization. Molecular weight of phytase protein was estimated about 42 kDa. On the other hand the model of phytase secondary structure showed the potential of thermostable characterization of enzymehttps://jab.uk.ac.ir/article_465_e5aa57d86a4dd8f90dbc33c8faf15c92.pdfphytase enzymegene cloningbacillus subtilisphytic aciderecombinant protein |
| spellingShingle | Hamid Arian nejad Mohammadreza Nassiri Aliasghar Aslaminejad Mojtaba Thmoors poor Reza valizadeh Ahmad Asoodeh SHahrokh GHovati Cloning, nucleotide characterization and modeling expression of phytase gene phyC from Bacillus subtilis مجله بیوتکنولوژی کشاورزی phytase enzyme gene cloning bacillus subtilis phytic acide recombinant protein |
| title | Cloning, nucleotide characterization and modeling expression of phytase gene phyC from Bacillus subtilis |
| title_full | Cloning, nucleotide characterization and modeling expression of phytase gene phyC from Bacillus subtilis |
| title_fullStr | Cloning, nucleotide characterization and modeling expression of phytase gene phyC from Bacillus subtilis |
| title_full_unstemmed | Cloning, nucleotide characterization and modeling expression of phytase gene phyC from Bacillus subtilis |
| title_short | Cloning, nucleotide characterization and modeling expression of phytase gene phyC from Bacillus subtilis |
| title_sort | cloning nucleotide characterization and modeling expression of phytase gene phyc from bacillus subtilis |
| topic | phytase enzyme gene cloning bacillus subtilis phytic acide recombinant protein |
| url | https://jab.uk.ac.ir/article_465_e5aa57d86a4dd8f90dbc33c8faf15c92.pdf |
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