Potential Effect of Glutamine in the Improvement of Intestinal Stem Cell Proliferation and the Alleviation of Burn-Induced Intestinal Injury via Activating YAP: A Preliminary Study
Burn injury is a common form of traumatic injury that leads to high mortality worldwide. A severe burn injury usually induces gut barrier dysfunction, partially resulting from the impairment in the proliferation and self-renewal of intestinal stem cells (ISCs) post burns. As a main energy substance...
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MDPI AG
2023-04-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/15/7/1766 |
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| author | Xia Chen Panyang Zhang Yajuan Zhang Shijun Fan Yan Wei Zhifan Yang Fengchao Wang Xi Peng |
| author_facet | Xia Chen Panyang Zhang Yajuan Zhang Shijun Fan Yan Wei Zhifan Yang Fengchao Wang Xi Peng |
| author_sort | Xia Chen |
| collection | DOAJ |
| description | Burn injury is a common form of traumatic injury that leads to high mortality worldwide. A severe burn injury usually induces gut barrier dysfunction, partially resulting from the impairment in the proliferation and self-renewal of intestinal stem cells (ISCs) post burns. As a main energy substance of small intestinal enterocytes, glutamine (Gln) is important for intestinal cell viability and growth, while its roles in ISCs-induced regeneration after burns are still unclear. To demonstrate the potential effects of Gln in improving ISCs proliferation and alleviating burn-induced intestinal injury, in this study, we verified that Gln significantly alleviated small intestine injury in burned mice model. It showed that Gln could significantly decrease the ferroptosis of crypt cells in the ileum, promote the proliferation of ISCs, and repair the crypt. These effects of Gln were also confirmed in the mouse small intestine organoids model. Further research found that Yes-associated protein (YAP) is suppressed after burn injury, and Gln could improve cell proliferation and accelerate the renewal of the damaged intestinal mucosal barrier after burns by activating YAP. YAP is closely associated with the changes in intestinal stem cell proliferation after burn injury and could be served as a potential target for severe burns. |
| first_indexed | 2024-03-11T05:28:12Z |
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| id | doaj.art-d71fa9b1337249e9a9203d578af33fd3 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-11T05:28:12Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
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| series | Nutrients |
| spelling | doaj.art-d71fa9b1337249e9a9203d578af33fd32023-11-17T17:21:44ZengMDPI AGNutrients2072-66432023-04-01157176610.3390/nu15071766Potential Effect of Glutamine in the Improvement of Intestinal Stem Cell Proliferation and the Alleviation of Burn-Induced Intestinal Injury via Activating YAP: A Preliminary StudyXia Chen0Panyang Zhang1Yajuan Zhang2Shijun Fan3Yan Wei4Zhifan Yang5Fengchao Wang6Xi Peng7Clinical Medical Research Center, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, ChinaClinical Medical Research Center, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, ChinaClinical Medical Research Center, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, ChinaClinical Medical Research Center, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, ChinaClinical Medical Research Center, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, ChinaInstitute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University (Army Medical University), Chongqing 400038, ChinaInstitute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University (Army Medical University), Chongqing 400038, ChinaClinical Medical Research Center, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, ChinaBurn injury is a common form of traumatic injury that leads to high mortality worldwide. A severe burn injury usually induces gut barrier dysfunction, partially resulting from the impairment in the proliferation and self-renewal of intestinal stem cells (ISCs) post burns. As a main energy substance of small intestinal enterocytes, glutamine (Gln) is important for intestinal cell viability and growth, while its roles in ISCs-induced regeneration after burns are still unclear. To demonstrate the potential effects of Gln in improving ISCs proliferation and alleviating burn-induced intestinal injury, in this study, we verified that Gln significantly alleviated small intestine injury in burned mice model. It showed that Gln could significantly decrease the ferroptosis of crypt cells in the ileum, promote the proliferation of ISCs, and repair the crypt. These effects of Gln were also confirmed in the mouse small intestine organoids model. Further research found that Yes-associated protein (YAP) is suppressed after burn injury, and Gln could improve cell proliferation and accelerate the renewal of the damaged intestinal mucosal barrier after burns by activating YAP. YAP is closely associated with the changes in intestinal stem cell proliferation after burn injury and could be served as a potential target for severe burns.https://www.mdpi.com/2072-6643/15/7/1766glutamineintestinal stem cellscryptproliferationburns |
| spellingShingle | Xia Chen Panyang Zhang Yajuan Zhang Shijun Fan Yan Wei Zhifan Yang Fengchao Wang Xi Peng Potential Effect of Glutamine in the Improvement of Intestinal Stem Cell Proliferation and the Alleviation of Burn-Induced Intestinal Injury via Activating YAP: A Preliminary Study Nutrients glutamine intestinal stem cells crypt proliferation burns |
| title | Potential Effect of Glutamine in the Improvement of Intestinal Stem Cell Proliferation and the Alleviation of Burn-Induced Intestinal Injury via Activating YAP: A Preliminary Study |
| title_full | Potential Effect of Glutamine in the Improvement of Intestinal Stem Cell Proliferation and the Alleviation of Burn-Induced Intestinal Injury via Activating YAP: A Preliminary Study |
| title_fullStr | Potential Effect of Glutamine in the Improvement of Intestinal Stem Cell Proliferation and the Alleviation of Burn-Induced Intestinal Injury via Activating YAP: A Preliminary Study |
| title_full_unstemmed | Potential Effect of Glutamine in the Improvement of Intestinal Stem Cell Proliferation and the Alleviation of Burn-Induced Intestinal Injury via Activating YAP: A Preliminary Study |
| title_short | Potential Effect of Glutamine in the Improvement of Intestinal Stem Cell Proliferation and the Alleviation of Burn-Induced Intestinal Injury via Activating YAP: A Preliminary Study |
| title_sort | potential effect of glutamine in the improvement of intestinal stem cell proliferation and the alleviation of burn induced intestinal injury via activating yap a preliminary study |
| topic | glutamine intestinal stem cells crypt proliferation burns |
| url | https://www.mdpi.com/2072-6643/15/7/1766 |
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