Development of a Simultaneous Analysis Method for Quality Control of a Traditional Herbal Formula, Daeshiho-Tang, Using 10 Marker Components
Daeshiho-tang (DSHT) is a traditional herbal formula consisting of six herbal medicines: <i>Bupleurum falcatum</i> L., <i>Scutellaria baicalensis</i> Georgi, <i>Paonia lactiflora</i> Pall., <i>Pheum palmatum</i> L., <i>Poncirus trifoliata</i&g...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/11/21/10242 |
| _version_ | 1797512811993104384 |
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| author | Chang-Seob Seo Hyeun-Kyoo Shin |
| author_facet | Chang-Seob Seo Hyeun-Kyoo Shin |
| author_sort | Chang-Seob Seo |
| collection | DOAJ |
| description | Daeshiho-tang (DSHT) is a traditional herbal formula consisting of six herbal medicines: <i>Bupleurum falcatum</i> L., <i>Scutellaria baicalensis</i> Georgi, <i>Paonia lactiflora</i> Pall., <i>Pheum palmatum</i> L., <i>Poncirus trifoliata</i> (L.) Raf., and <i>Pinellia ternate</i> (Thunb.) Makino. In this study, we developed a simultaneous analysis method based on high-performance liquid chromatography for the quality control of DSHT. Chromatographic separation of 10 marker components (gallic acid, albiflorin, paeoniflorin, naringin, benzoic acid, baicalin, poncirin, wogonoside, baicalein, and wogonin) was achieved using a water–acetonitrile system as the mobile phase with a SunFire C18 reversed-phase column. The developed analytical method was validated with respect to linearity, limit of detection, limit of quantitation, recovery, and precision. Among the 10 markers of DSHT in the established assay, baicalin, the main compound of <i>Scutellaria baicalensis</i> Georgi, was present in the highest concentration (36.86–46.17 mg/g). The validated assay will be useful for the quality control of DSHT. |
| first_indexed | 2024-03-10T06:06:57Z |
| format | Article |
| id | doaj.art-d721740acad64cd3b97e5444421bfe96 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T06:06:57Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-d721740acad64cd3b97e5444421bfe962023-11-22T20:29:55ZengMDPI AGApplied Sciences2076-34172021-11-0111211024210.3390/app112110242Development of a Simultaneous Analysis Method for Quality Control of a Traditional Herbal Formula, Daeshiho-Tang, Using 10 Marker ComponentsChang-Seob Seo0Hyeun-Kyoo Shin1KM Science Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, KoreaKM Science Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, KoreaDaeshiho-tang (DSHT) is a traditional herbal formula consisting of six herbal medicines: <i>Bupleurum falcatum</i> L., <i>Scutellaria baicalensis</i> Georgi, <i>Paonia lactiflora</i> Pall., <i>Pheum palmatum</i> L., <i>Poncirus trifoliata</i> (L.) Raf., and <i>Pinellia ternate</i> (Thunb.) Makino. In this study, we developed a simultaneous analysis method based on high-performance liquid chromatography for the quality control of DSHT. Chromatographic separation of 10 marker components (gallic acid, albiflorin, paeoniflorin, naringin, benzoic acid, baicalin, poncirin, wogonoside, baicalein, and wogonin) was achieved using a water–acetonitrile system as the mobile phase with a SunFire C18 reversed-phase column. The developed analytical method was validated with respect to linearity, limit of detection, limit of quantitation, recovery, and precision. Among the 10 markers of DSHT in the established assay, baicalin, the main compound of <i>Scutellaria baicalensis</i> Georgi, was present in the highest concentration (36.86–46.17 mg/g). The validated assay will be useful for the quality control of DSHT.https://www.mdpi.com/2076-3417/11/21/10242simultaneous analysisquality controltraditional herbal formuladaeshiho-tang |
| spellingShingle | Chang-Seob Seo Hyeun-Kyoo Shin Development of a Simultaneous Analysis Method for Quality Control of a Traditional Herbal Formula, Daeshiho-Tang, Using 10 Marker Components Applied Sciences simultaneous analysis quality control traditional herbal formula daeshiho-tang |
| title | Development of a Simultaneous Analysis Method for Quality Control of a Traditional Herbal Formula, Daeshiho-Tang, Using 10 Marker Components |
| title_full | Development of a Simultaneous Analysis Method for Quality Control of a Traditional Herbal Formula, Daeshiho-Tang, Using 10 Marker Components |
| title_fullStr | Development of a Simultaneous Analysis Method for Quality Control of a Traditional Herbal Formula, Daeshiho-Tang, Using 10 Marker Components |
| title_full_unstemmed | Development of a Simultaneous Analysis Method for Quality Control of a Traditional Herbal Formula, Daeshiho-Tang, Using 10 Marker Components |
| title_short | Development of a Simultaneous Analysis Method for Quality Control of a Traditional Herbal Formula, Daeshiho-Tang, Using 10 Marker Components |
| title_sort | development of a simultaneous analysis method for quality control of a traditional herbal formula daeshiho tang using 10 marker components |
| topic | simultaneous analysis quality control traditional herbal formula daeshiho-tang |
| url | https://www.mdpi.com/2076-3417/11/21/10242 |
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