Pre-treatment by Rosemary Extract or Cell Transplantation Improves Memory Deficits of Parkinson’s Disease: When Tradition Meets the Future
ABSTRACT The therapeutic effect of adipose tissue-derived stem cells (ADSCs) or RE on hippocampal neurogenesis and memory in Parkinsonian rats were investigated. Male rats were lesioned by bilateral intra-nigral injections of 6-OHDA and divided into six groups: 1. Lesion 2 and 3: RE and water groups...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2021-05-01
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| Series: | Brazilian Archives of Biology and Technology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000100306&tlng=en |
| _version_ | 1798020057194823680 |
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| author | Seyyede Narjes Hashemi Rad Maryam Haji Ghasem Kashani Kataneh Abrari |
| author_facet | Seyyede Narjes Hashemi Rad Maryam Haji Ghasem Kashani Kataneh Abrari |
| author_sort | Seyyede Narjes Hashemi Rad |
| collection | DOAJ |
| description | ABSTRACT The therapeutic effect of adipose tissue-derived stem cells (ADSCs) or RE on hippocampal neurogenesis and memory in Parkinsonian rats were investigated. Male rats were lesioned by bilateral intra-nigral injections of 6-OHDA and divided into six groups: 1. Lesion 2 and 3: RE and water groups were lesioned rats pretreated with RE or water, from 2weeks before neurotoxin injection and treated once a day for 8weeks post lesion. 4&5: Cell and α-MEM (α-minimal essential médium) received intravenous injection of BrdU-labeled ADSCs or medium, respectively from 10days post lesion until 8weeks later. 6: Sham was injected by saline instead of neurotoxin. Memory was assessed using Morris water Maze (MWM), one week before and at 1, 4 and 8weeks post 6-OHDA lesion. After the last probe, the animals were sacrificed and brain tissue obtained. Paraffin sections were stained using cresyl violet, anti-BrdU (Bromodeoxyuridine / 5-bromo-2'-deoxyuridine), anti-GFAP (Glial fibrillary acidic protein) and anti-TH antibodies. There was a significant difference of time spent in the target quadrant between groups during probe trial at 4 and 8 weeks’ post- lesion. Cell and RE groups spent a significantly longer period in the target quadrant and had lower latency as compared with lesion. Treated groups have a significantly higher neuronal density in hippocampus compared to water, α-MEM and lesion groups. BrdU positive cells were presented in lesioned sites. The GFAP (Glial fibrillary acidic protein) positive cells were reduced in treated and sham groups compared to the water, α-MEM and lesion groups. Oral administration of RE (Rosemary extract) or ADSCs injection could improve memory deficit in the Parkinsonian rat by neuroprotection. |
| first_indexed | 2024-04-11T16:51:30Z |
| format | Article |
| id | doaj.art-d72bc68345034278bcb2fb3d9388234a |
| institution | Directory Open Access Journal |
| issn | 1678-4324 |
| language | English |
| last_indexed | 2024-04-11T16:51:30Z |
| publishDate | 2021-05-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-d72bc68345034278bcb2fb3d9388234a2022-12-22T04:13:26ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242021-05-016410.1590/1678-4324-2021180392Pre-treatment by Rosemary Extract or Cell Transplantation Improves Memory Deficits of Parkinson’s Disease: When Tradition Meets the FutureSeyyede Narjes Hashemi Radhttps://orcid.org/0000-0002-2889-9893Maryam Haji Ghasem Kashanihttps://orcid.org/0000-0001-8889-6427Kataneh Abrarihttps://orcid.org/0000-0002-8056-9218ABSTRACT The therapeutic effect of adipose tissue-derived stem cells (ADSCs) or RE on hippocampal neurogenesis and memory in Parkinsonian rats were investigated. Male rats were lesioned by bilateral intra-nigral injections of 6-OHDA and divided into six groups: 1. Lesion 2 and 3: RE and water groups were lesioned rats pretreated with RE or water, from 2weeks before neurotoxin injection and treated once a day for 8weeks post lesion. 4&5: Cell and α-MEM (α-minimal essential médium) received intravenous injection of BrdU-labeled ADSCs or medium, respectively from 10days post lesion until 8weeks later. 6: Sham was injected by saline instead of neurotoxin. Memory was assessed using Morris water Maze (MWM), one week before and at 1, 4 and 8weeks post 6-OHDA lesion. After the last probe, the animals were sacrificed and brain tissue obtained. Paraffin sections were stained using cresyl violet, anti-BrdU (Bromodeoxyuridine / 5-bromo-2'-deoxyuridine), anti-GFAP (Glial fibrillary acidic protein) and anti-TH antibodies. There was a significant difference of time spent in the target quadrant between groups during probe trial at 4 and 8 weeks’ post- lesion. Cell and RE groups spent a significantly longer period in the target quadrant and had lower latency as compared with lesion. Treated groups have a significantly higher neuronal density in hippocampus compared to water, α-MEM and lesion groups. BrdU positive cells were presented in lesioned sites. The GFAP (Glial fibrillary acidic protein) positive cells were reduced in treated and sham groups compared to the water, α-MEM and lesion groups. Oral administration of RE (Rosemary extract) or ADSCs injection could improve memory deficit in the Parkinsonian rat by neuroprotection.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000100306&tlng=enRosemary extractcell transplantationhippocampusmemory |
| spellingShingle | Seyyede Narjes Hashemi Rad Maryam Haji Ghasem Kashani Kataneh Abrari Pre-treatment by Rosemary Extract or Cell Transplantation Improves Memory Deficits of Parkinson’s Disease: When Tradition Meets the Future Brazilian Archives of Biology and Technology Rosemary extract cell transplantation hippocampus memory |
| title | Pre-treatment by Rosemary Extract or Cell Transplantation Improves Memory Deficits of Parkinson’s Disease: When Tradition Meets the Future |
| title_full | Pre-treatment by Rosemary Extract or Cell Transplantation Improves Memory Deficits of Parkinson’s Disease: When Tradition Meets the Future |
| title_fullStr | Pre-treatment by Rosemary Extract or Cell Transplantation Improves Memory Deficits of Parkinson’s Disease: When Tradition Meets the Future |
| title_full_unstemmed | Pre-treatment by Rosemary Extract or Cell Transplantation Improves Memory Deficits of Parkinson’s Disease: When Tradition Meets the Future |
| title_short | Pre-treatment by Rosemary Extract or Cell Transplantation Improves Memory Deficits of Parkinson’s Disease: When Tradition Meets the Future |
| title_sort | pre treatment by rosemary extract or cell transplantation improves memory deficits of parkinson s disease when tradition meets the future |
| topic | Rosemary extract cell transplantation hippocampus memory |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000100306&tlng=en |
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