Isolation and Detection of Erysipelothrix rhusiopathiae and Its Distribution in Humans and Animals by Phenotypical and Molecular Methods in Ahvaz-Iran in 2015
Background: Erysipelothrix rhusiopathiae (E. rhusiopathiae) is generally transmitted into the gastrointestinal tract of animals by the intake of contaminated food or water and causes great economic loss in agriculture worldwide. Some of the Erysipelothrix spp. are the causative agents of erysipeloid...
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2017-07-01
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| Series: | Iranian Journal of Medical Sciences |
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| Online Access: | http://ijms.sums.ac.ir/index.php/IJMS/article/view/2988 |
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| author | Pariya Ahmadi Balootaki Mansour Amin Farkhondeh Haghparasti Farokh Rokhbakhsh-Zamin |
| author_facet | Pariya Ahmadi Balootaki Mansour Amin Farkhondeh Haghparasti Farokh Rokhbakhsh-Zamin |
| author_sort | Pariya Ahmadi Balootaki |
| collection | DOAJ |
| description | Background: Erysipelothrix rhusiopathiae (E. rhusiopathiae) is generally transmitted into the gastrointestinal tract of animals by the intake of contaminated food or water and causes great economic loss in agriculture worldwide. Some of the Erysipelothrix spp. are the causative agents of erysipeloid, which is an occupational infection in humans. The aim of the present study was to isolate E. rhusiopathiae from animals as well as the hands of the butchers working in Ahvaz, Iran, and to determine their susceptibility to antibiotics.
Methods: Totally, 150 samples were taken from slaughterhouse workers, fishermen, and livers and hearts of sheep and calves by the swabbing method. Phenotypical methods and polymerase chain reaction (PCR) were used for the isolation and identification of E. rhusiopathiae. The isolates were tested for their susceptibility to commonly used antimicrobial agents using the disk diffusion protocol described by the Clinical and Laboratory Standards Institute.
Results: Out of the 150 samples examined via phenotypical and biochemical tests, 16 samples were positive as putative Erysipelothrix spp. twelve cases out of the 16 putative Erysipelothrix spp. were confirmed by PCR. The tested isolates were highly sensitive to the antibiotics used. The results of the sensitivity and specificity of PCR revealed that the sensitivity and specificity of indirect PCR were higher than those of direct PCR.
Conclusion: E. rhusiopathiae is widely distributed on seafood and presents as a commensal pathogen in nature and animals. Infection with this microorganism should be emphasized because it is a rare organism causing severe infections such as infectious endocarditis and polyarthritis following localized infections. |
| first_indexed | 2024-12-11T22:22:47Z |
| format | Article |
| id | doaj.art-d7357b76d95845ef8b524295eea5e14c |
| institution | Directory Open Access Journal |
| issn | 0253-0716 1735-3688 |
| language | English |
| last_indexed | 2024-12-11T22:22:47Z |
| publishDate | 2017-07-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Medical Sciences |
| spelling | doaj.art-d7357b76d95845ef8b524295eea5e14c2022-12-22T00:48:24ZengShiraz University of Medical SciencesIranian Journal of Medical Sciences0253-07161735-36882017-07-01424377383Isolation and Detection of Erysipelothrix rhusiopathiae and Its Distribution in Humans and Animals by Phenotypical and Molecular Methods in Ahvaz-Iran in 2015Pariya Ahmadi Balootaki0Mansour Amin1Farkhondeh Haghparasti2Farokh Rokhbakhsh-Zamin3Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran; and Department of Microbiology, Kerman Science and Research Branch, Islamic Azad University, Kerman, IraHealth Research Institute, Infectious and Tropical Diseases Research Center and Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranHealth Research Institute, Infectious and Tropical Diseases Research Center and Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranDepartment of Microbiology, Kerman Branch, Islamic Azad University, Kerman, IranBackground: Erysipelothrix rhusiopathiae (E. rhusiopathiae) is generally transmitted into the gastrointestinal tract of animals by the intake of contaminated food or water and causes great economic loss in agriculture worldwide. Some of the Erysipelothrix spp. are the causative agents of erysipeloid, which is an occupational infection in humans. The aim of the present study was to isolate E. rhusiopathiae from animals as well as the hands of the butchers working in Ahvaz, Iran, and to determine their susceptibility to antibiotics. Methods: Totally, 150 samples were taken from slaughterhouse workers, fishermen, and livers and hearts of sheep and calves by the swabbing method. Phenotypical methods and polymerase chain reaction (PCR) were used for the isolation and identification of E. rhusiopathiae. The isolates were tested for their susceptibility to commonly used antimicrobial agents using the disk diffusion protocol described by the Clinical and Laboratory Standards Institute. Results: Out of the 150 samples examined via phenotypical and biochemical tests, 16 samples were positive as putative Erysipelothrix spp. twelve cases out of the 16 putative Erysipelothrix spp. were confirmed by PCR. The tested isolates were highly sensitive to the antibiotics used. The results of the sensitivity and specificity of PCR revealed that the sensitivity and specificity of indirect PCR were higher than those of direct PCR. Conclusion: E. rhusiopathiae is widely distributed on seafood and presents as a commensal pathogen in nature and animals. Infection with this microorganism should be emphasized because it is a rare organism causing severe infections such as infectious endocarditis and polyarthritis following localized infections.http://ijms.sums.ac.ir/index.php/IJMS/article/view/2988Erysipelothrix rhusiopathiaeErysipeloidOccupational diseasesPolymerase chain reaction |
| spellingShingle | Pariya Ahmadi Balootaki Mansour Amin Farkhondeh Haghparasti Farokh Rokhbakhsh-Zamin Isolation and Detection of Erysipelothrix rhusiopathiae and Its Distribution in Humans and Animals by Phenotypical and Molecular Methods in Ahvaz-Iran in 2015 Iranian Journal of Medical Sciences Erysipelothrix rhusiopathiae Erysipeloid Occupational diseases Polymerase chain reaction |
| title | Isolation and Detection of Erysipelothrix rhusiopathiae and Its Distribution in Humans and Animals by Phenotypical and Molecular Methods in Ahvaz-Iran in 2015 |
| title_full | Isolation and Detection of Erysipelothrix rhusiopathiae and Its Distribution in Humans and Animals by Phenotypical and Molecular Methods in Ahvaz-Iran in 2015 |
| title_fullStr | Isolation and Detection of Erysipelothrix rhusiopathiae and Its Distribution in Humans and Animals by Phenotypical and Molecular Methods in Ahvaz-Iran in 2015 |
| title_full_unstemmed | Isolation and Detection of Erysipelothrix rhusiopathiae and Its Distribution in Humans and Animals by Phenotypical and Molecular Methods in Ahvaz-Iran in 2015 |
| title_short | Isolation and Detection of Erysipelothrix rhusiopathiae and Its Distribution in Humans and Animals by Phenotypical and Molecular Methods in Ahvaz-Iran in 2015 |
| title_sort | isolation and detection of erysipelothrix rhusiopathiae and its distribution in humans and animals by phenotypical and molecular methods in ahvaz iran in 2015 |
| topic | Erysipelothrix rhusiopathiae Erysipeloid Occupational diseases Polymerase chain reaction |
| url | http://ijms.sums.ac.ir/index.php/IJMS/article/view/2988 |
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