Detection and Quantification of Klebsiella pneumoniae in Fecal Samples Using Digital Droplet PCR in Comparison with Real-Time PCR

Detection and Quantification of Klebsiella pneumoniae in Fecal Samples Using Digital Droplet PCR in Comparison with Real-Time PCR

ABSTRACT This study aimed to develop a rapid and sensitive droplet digital PCR (ddPCR) assay for the specific detection of Klebsiella pneumoniae in fecal samples, and to evaluate its application in the clinic by comparison with real-time PCR assay and conventional microbial culture. Specific primers...

Full description

Bibliographic Details
Main Authors: Junxia Feng, Xiaohu Cui, Bing Du, Hanqing Zhao, Yanling Feng, Jinghua Cui, Chao Yan, Lin Gan, Zheng Fan, Tongtong Fu, Ziying Xu, Zihui Yu, Rui Zhang, Shuheng Du, Ziyan Tian, Qun Zhang, Guanhua Xue, Jing Yuan
Format: Article
Language:English
Published: American Society for Microbiology 2023-08-01
Series:Microbiology Spectrum
Subjects:
droplet digital PCR
real-time PCR
Klebsiella pneumoniae
quantification
Online Access:https://journals.asm.org/doi/10.1128/spectrum.04249-22

Internet

https://journals.asm.org/doi/10.1128/spectrum.04249-22

Similar Items