Inhibitory effects of epigallocatechin-3-gallate on the pathogenic properties of P. gingivalis in vitro
Objective To explore the antibacterial activity of epigallocatechin-3-gallate (EGCG) on P. gingivalis and the inhibitory effects on matrix metalloproteinases (MMPs) production induced by P. gingivalis. Methods The antimicrobial effect of EGCG against planktonic cultures and biofilms of P. gingivalis...
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| Format: | Article |
| Language: | zho |
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Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases
2021-05-01
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| Series: | 口腔疾病防治 |
| Subjects: | |
| Online Access: | http://www.kqjbfz.com/CN/10.12016/j.issn.2096-1456.2021.05.004 |
| _version_ | 1819159987701678080 |
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| author | QI Xia KONG Lingxue LI Shujuan MA Siting QI Yali ZHAO Lei |
| author_facet | QI Xia KONG Lingxue LI Shujuan MA Siting QI Yali ZHAO Lei |
| author_sort | QI Xia |
| collection | DOAJ |
| description | Objective To explore the antibacterial activity of epigallocatechin-3-gallate (EGCG) on P. gingivalis and the inhibitory effects on matrix metalloproteinases (MMPs) production induced by P. gingivalis. Methods The antimicrobial effect of EGCG against planktonic cultures and biofilms of P. gingivalis was evaluated using microplate dilution assays. The microstructural changes in biofilms were studied using scanning electron microscopy (SEM). The inhibitory effect of EGCG on arginine gingipain (Rgp) and lysine gingipain (Kgp) activity of P. gingivalis was evaluated using synthetic chromogenic peptides and fluorogenic substrates. Enzyme-linked immunosorbent assay (ELISA) and qRT-PCR analysis were used to assess MMP-1 and MMP-2 mRNA expression and secretion by human gingival fibroblasts (HGFs) stimulated with P. gingivalis in the presence or absence of EGCG, respectively. Results The MIC and MBC of EGCG against P. gingivalis were 62.5 μg/mL and 500 μg/mL, respectively. EGCG can not only inhibit the biofilm formation of P. gingivalis but also has a scavenging effect on mature biofilms and can affect their viability. Additionally, 10 μg/mL and 50 μg/mL of EGCG inhibited the proteinase activities of Rgp and Kgp, respectively (P < 0.05). Finally, the mRNA expression and secretion of MMP-1 and MMP-2 by HGFs stimulated by P. gingivalis were significantly inhibited by 50 μg/mL of EGCG (P < 0.05). Conclusion EGCG exhibits antimicrobial effects against P. gingivalis and reduces the expression of MMPs by HGFs. |
| first_indexed | 2024-12-22T16:49:17Z |
| format | Article |
| id | doaj.art-d7383ad53f9f4caba75a72a2680a220c |
| institution | Directory Open Access Journal |
| issn | 2096-1456 2096-1456 |
| language | zho |
| last_indexed | 2024-12-22T16:49:17Z |
| publishDate | 2021-05-01 |
| publisher | Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases |
| record_format | Article |
| series | 口腔疾病防治 |
| spelling | doaj.art-d7383ad53f9f4caba75a72a2680a220c2022-12-21T18:19:38ZzhoEditorial Department of Journal of Prevention and Treatment for Stomatological Diseases口腔疾病防治2096-14562096-14562021-05-0129531432110.12016/j.issn.2096⁃1456.2021.05.004Inhibitory effects of epigallocatechin-3-gallate on the pathogenic properties of P. gingivalis in vitroQI Xia0 KONG Lingxue1 LI Shujuan2MA Siting3QI Yali4ZHAO Lei5Department of Periodontics, School and Hospital of Stomatology, Hebei Medical University, Hebei Key Laboratory of Stomatology,Hebei Clinical Research Center for Oral DiseasesDepartment of Periodontics and Oral Mucosa, Jinan Stomatologic HospitalDepartment of Periodontics, School and Hospital of Stomatology, Hebei Medical University, Hebei Key Laboratory of Stomatology,Hebei Clinical Research Center for Oral DiseasesDepartment of Periodontics, School and Hospital of Stomatology, Hebei Medical University, Hebei Key Laboratory of Stomatology,Hebei Clinical Research Center for Oral DiseasesDepartment of Periodontics, School and Hospital of Stomatology, Hebei Medical University, Hebei Key Laboratory of Stomatology,Hebei Clinical Research Center for Oral DiseasesState Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, Department of Periodontics, West China Hospital of Stomatology, Sichuan UniversityObjective To explore the antibacterial activity of epigallocatechin-3-gallate (EGCG) on P. gingivalis and the inhibitory effects on matrix metalloproteinases (MMPs) production induced by P. gingivalis. Methods The antimicrobial effect of EGCG against planktonic cultures and biofilms of P. gingivalis was evaluated using microplate dilution assays. The microstructural changes in biofilms were studied using scanning electron microscopy (SEM). The inhibitory effect of EGCG on arginine gingipain (Rgp) and lysine gingipain (Kgp) activity of P. gingivalis was evaluated using synthetic chromogenic peptides and fluorogenic substrates. Enzyme-linked immunosorbent assay (ELISA) and qRT-PCR analysis were used to assess MMP-1 and MMP-2 mRNA expression and secretion by human gingival fibroblasts (HGFs) stimulated with P. gingivalis in the presence or absence of EGCG, respectively. Results The MIC and MBC of EGCG against P. gingivalis were 62.5 μg/mL and 500 μg/mL, respectively. EGCG can not only inhibit the biofilm formation of P. gingivalis but also has a scavenging effect on mature biofilms and can affect their viability. Additionally, 10 μg/mL and 50 μg/mL of EGCG inhibited the proteinase activities of Rgp and Kgp, respectively (P < 0.05). Finally, the mRNA expression and secretion of MMP-1 and MMP-2 by HGFs stimulated by P. gingivalis were significantly inhibited by 50 μg/mL of EGCG (P < 0.05). Conclusion EGCG exhibits antimicrobial effects against P. gingivalis and reduces the expression of MMPs by HGFs.http://www.kqjbfz.com/CN/10.12016/j.issn.2096-1456.2021.05.004epigallocatechin-3-gallateporphyromonas gingivalisbiofilm formationbiofilm reductionarginine gingipainlysine gingipainantibacterialanti-inflammatorygingival fibroblastsmatrix metalloproteinase |
| spellingShingle | QI Xia KONG Lingxue LI Shujuan MA Siting QI Yali ZHAO Lei Inhibitory effects of epigallocatechin-3-gallate on the pathogenic properties of P. gingivalis in vitro 口腔疾病防治 epigallocatechin-3-gallate porphyromonas gingivalis biofilm formation biofilm reduction arginine gingipain lysine gingipain antibacterial anti-inflammatory gingival fibroblasts matrix metalloproteinase |
| title | Inhibitory effects of epigallocatechin-3-gallate on the pathogenic properties of P. gingivalis in vitro |
| title_full | Inhibitory effects of epigallocatechin-3-gallate on the pathogenic properties of P. gingivalis in vitro |
| title_fullStr | Inhibitory effects of epigallocatechin-3-gallate on the pathogenic properties of P. gingivalis in vitro |
| title_full_unstemmed | Inhibitory effects of epigallocatechin-3-gallate on the pathogenic properties of P. gingivalis in vitro |
| title_short | Inhibitory effects of epigallocatechin-3-gallate on the pathogenic properties of P. gingivalis in vitro |
| title_sort | inhibitory effects of epigallocatechin 3 gallate on the pathogenic properties of p gingivalis in vitro |
| topic | epigallocatechin-3-gallate porphyromonas gingivalis biofilm formation biofilm reduction arginine gingipain lysine gingipain antibacterial anti-inflammatory gingival fibroblasts matrix metalloproteinase |
| url | http://www.kqjbfz.com/CN/10.12016/j.issn.2096-1456.2021.05.004 |
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