Overexpression of <i>AcEXPA23</i> Promotes Lateral Root Development in Kiwifruit
Kiwifruit is loved by consumers for its unique taste and rich vitamin C content. Kiwifruit are very sensitive to adverse soil environments owing to fleshy and shallow roots, which limits the uptake of water and nutrients into the root system, resulting in low yield and poor fruit quality. Lateral ro...
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MDPI AG
2022-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/14/8026 |
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| author | Zhiyong Wu Ming Li Yunpeng Zhong Lan Li Dawei Cheng Hong Gu Xizhi Guo Xiujuan Qi Jinyong Chen |
| author_facet | Zhiyong Wu Ming Li Yunpeng Zhong Lan Li Dawei Cheng Hong Gu Xizhi Guo Xiujuan Qi Jinyong Chen |
| author_sort | Zhiyong Wu |
| collection | DOAJ |
| description | Kiwifruit is loved by consumers for its unique taste and rich vitamin C content. Kiwifruit are very sensitive to adverse soil environments owing to fleshy and shallow roots, which limits the uptake of water and nutrients into the root system, resulting in low yield and poor fruit quality. Lateral roots are the key organs for plants to absorb water and nutrients. Improving water and fertilizer use efficiency by promoting lateral root development is a feasible method to improve yield and quality. Expansin proteins plays a major role in lateral root growth; hence, it is important to identify expansin protein family members, screen key genes, and explore gene function in root development. In this study, 41 expansin genes were identified based on the genome of kiwifruit (‘Hongyang’, <i>Actinidia chinensis</i>). By clustering with the <i>Arabidopsis thaliana</i> expansin protein family, the 41 AcExpansin proteins were divided into four subfamilies. The AcExpansin protein family was further analysed by bioinformatics methods and was shown to be evolutionarily diverse and conserved at the DNA and protein levels. Based on previous transcriptome data and quantitative real-time PCR assays, we screened the candidate gene <i>AcEXPA23</i>. Overexpression of <i>AcEXPA23</i> in kiwifruit increased the number of kiwifruit lateral roots. |
| first_indexed | 2024-03-09T10:17:46Z |
| format | Article |
| id | doaj.art-d73a87c07dd74c239dc154000bbffae2 |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T10:17:46Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-d73a87c07dd74c239dc154000bbffae22023-12-01T22:16:38ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-07-012314802610.3390/ijms23148026Overexpression of <i>AcEXPA23</i> Promotes Lateral Root Development in KiwifruitZhiyong Wu0Ming Li1Yunpeng Zhong2Lan Li3Dawei Cheng4Hong Gu5Xizhi Guo6Xiujuan Qi7Jinyong Chen8Key Laboratory of Fruit Breeding Technology of Ministry of Agriculture and Rural Affairs, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaKey Laboratory of Fruit Breeding Technology of Ministry of Agriculture and Rural Affairs, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaKey Laboratory of Fruit Breeding Technology of Ministry of Agriculture and Rural Affairs, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaKey Laboratory of Fruit Breeding Technology of Ministry of Agriculture and Rural Affairs, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaKey Laboratory of Fruit Breeding Technology of Ministry of Agriculture and Rural Affairs, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaKey Laboratory of Fruit Breeding Technology of Ministry of Agriculture and Rural Affairs, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaKey Laboratory of Fruit Breeding Technology of Ministry of Agriculture and Rural Affairs, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaKey Laboratory of Fruit Breeding Technology of Ministry of Agriculture and Rural Affairs, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaKey Laboratory of Fruit Breeding Technology of Ministry of Agriculture and Rural Affairs, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaKiwifruit is loved by consumers for its unique taste and rich vitamin C content. Kiwifruit are very sensitive to adverse soil environments owing to fleshy and shallow roots, which limits the uptake of water and nutrients into the root system, resulting in low yield and poor fruit quality. Lateral roots are the key organs for plants to absorb water and nutrients. Improving water and fertilizer use efficiency by promoting lateral root development is a feasible method to improve yield and quality. Expansin proteins plays a major role in lateral root growth; hence, it is important to identify expansin protein family members, screen key genes, and explore gene function in root development. In this study, 41 expansin genes were identified based on the genome of kiwifruit (‘Hongyang’, <i>Actinidia chinensis</i>). By clustering with the <i>Arabidopsis thaliana</i> expansin protein family, the 41 AcExpansin proteins were divided into four subfamilies. The AcExpansin protein family was further analysed by bioinformatics methods and was shown to be evolutionarily diverse and conserved at the DNA and protein levels. Based on previous transcriptome data and quantitative real-time PCR assays, we screened the candidate gene <i>AcEXPA23</i>. Overexpression of <i>AcEXPA23</i> in kiwifruit increased the number of kiwifruit lateral roots.https://www.mdpi.com/1422-0067/23/14/8026kiwifruitexpansinlateral root |
| spellingShingle | Zhiyong Wu Ming Li Yunpeng Zhong Lan Li Dawei Cheng Hong Gu Xizhi Guo Xiujuan Qi Jinyong Chen Overexpression of <i>AcEXPA23</i> Promotes Lateral Root Development in Kiwifruit International Journal of Molecular Sciences kiwifruit expansin lateral root |
| title | Overexpression of <i>AcEXPA23</i> Promotes Lateral Root Development in Kiwifruit |
| title_full | Overexpression of <i>AcEXPA23</i> Promotes Lateral Root Development in Kiwifruit |
| title_fullStr | Overexpression of <i>AcEXPA23</i> Promotes Lateral Root Development in Kiwifruit |
| title_full_unstemmed | Overexpression of <i>AcEXPA23</i> Promotes Lateral Root Development in Kiwifruit |
| title_short | Overexpression of <i>AcEXPA23</i> Promotes Lateral Root Development in Kiwifruit |
| title_sort | overexpression of i acexpa23 i promotes lateral root development in kiwifruit |
| topic | kiwifruit expansin lateral root |
| url | https://www.mdpi.com/1422-0067/23/14/8026 |
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