Development and validation of a GC-MS method for determination of amphetamine-type stimulants and ketamine in human hair
A solid-phase extraction (SPE) followed by gas chromatography-mass spectrometry (GC-MS) method was developed and validated for determination of seven amphetamine-type stimulants (ATSs) including amphetamine (AM), methamphetamine (MA), 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphet...
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| Format: | Article |
| Language: | English |
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Faculty of Pharmacy, Mahidol University
2023-01-01
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| Series: | Pharmaceutical Sciences Asia |
| Subjects: | |
| Online Access: | https://pharmacy.mahidol.ac.th/journal/_files/2023-50-1_4.pdf |
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| author | Tran Viet Hung Ha Minh Hien Pham Quoc Chinh Pham Thi Thu Ha Nguyen Duc Nhu Nguyen Duc Tuan |
| author_facet | Tran Viet Hung Ha Minh Hien Pham Quoc Chinh Pham Thi Thu Ha Nguyen Duc Nhu Nguyen Duc Tuan |
| author_sort | Tran Viet Hung |
| collection | DOAJ |
| description | A solid-phase extraction (SPE) followed by gas chromatography-mass spectrometry (GC-MS) method was developed and validated for determination of seven amphetamine-type stimulants (ATSs) including amphetamine (AM), methamphetamine (MA), 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylendioxyethylamphetamine (MDEA), para-methoxyamphetamine (PMA), 4-bromo-2,5-
dimethoxyamphetamine (DOB), and ketamine (KET), norketamine (NKT) in human hair. Ten milligrams of human hair was washed by water and methanol followed by soaking in a solution of 1% hydrochloric acid in methanol for 18 hours. The analytes from the methanolic extract were isolated by a SPE procedure before being derivatized using heptafluorobutyric anhydride (HFBA) at 80°C for 40 minutes. The selected ion monitoring (SIM) method was used for the quantification of the derivatized compounds. The linear range was from 0.5-40 ng/mg for all of the analytes with the coefficient of determination (R2)>0.9971. The intra-day and inter-day accuracies were in the range of 93.63-112.40% and 94.70-110.20%, respectively. The intra-day and inter-day precision (RSD%) were in the range of 8.72 and 9.73, respectively. Limit of detection (LOD) and limit of quantitation (LOQ) for each analyte were less than 0.10 and 0.32 ng/mg, respectively. The recoveries were in the range of 75.18-89.30%. The GC-MS was used for the hair analysis on 51 subjects suspected to be ATSs and ketamine user. The average content of all the analytes was in the range of 1.14-12.70 ng/mg. On the basis of these results, the method was proved to be effective for identification and quantitation of |
| first_indexed | 2024-04-10T21:33:11Z |
| format | Article |
| id | doaj.art-d73bb044989040de8e3f1b8d20138f8a |
| institution | Directory Open Access Journal |
| issn | 2586-8470 |
| language | English |
| last_indexed | 2024-04-10T21:33:11Z |
| publishDate | 2023-01-01 |
| publisher | Faculty of Pharmacy, Mahidol University |
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| series | Pharmaceutical Sciences Asia |
| spelling | doaj.art-d73bb044989040de8e3f1b8d20138f8a2023-01-19T12:57:19ZengFaculty of Pharmacy, Mahidol UniversityPharmaceutical Sciences Asia2586-84702023-01-01501243310.29090/psa.2023.01.22.292Development and validation of a GC-MS method for determination of amphetamine-type stimulants and ketamine in human hairTran Viet Hung0Ha Minh Hien1Pham Quoc Chinh2Pham Thi Thu Ha3Nguyen Duc Nhu4Nguyen Duc Tuan5Institute of Drug Quality Control Ho Chi Minh City, Ho Chi Minh City, VietnamInstitute of Drug Quality Control Ho Chi Minh City, Ho Chi Minh City, VietnamNational Institute of Forensic Medicine, Hanoi City, VietnamNational Institute of Forensic Medicine, Hanoi City, VietnamNational Institute of Forensic Medicine, Hanoi City, VietnamFaculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, VietnamA solid-phase extraction (SPE) followed by gas chromatography-mass spectrometry (GC-MS) method was developed and validated for determination of seven amphetamine-type stimulants (ATSs) including amphetamine (AM), methamphetamine (MA), 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylendioxyethylamphetamine (MDEA), para-methoxyamphetamine (PMA), 4-bromo-2,5- dimethoxyamphetamine (DOB), and ketamine (KET), norketamine (NKT) in human hair. Ten milligrams of human hair was washed by water and methanol followed by soaking in a solution of 1% hydrochloric acid in methanol for 18 hours. The analytes from the methanolic extract were isolated by a SPE procedure before being derivatized using heptafluorobutyric anhydride (HFBA) at 80°C for 40 minutes. The selected ion monitoring (SIM) method was used for the quantification of the derivatized compounds. The linear range was from 0.5-40 ng/mg for all of the analytes with the coefficient of determination (R2)>0.9971. The intra-day and inter-day accuracies were in the range of 93.63-112.40% and 94.70-110.20%, respectively. The intra-day and inter-day precision (RSD%) were in the range of 8.72 and 9.73, respectively. Limit of detection (LOD) and limit of quantitation (LOQ) for each analyte were less than 0.10 and 0.32 ng/mg, respectively. The recoveries were in the range of 75.18-89.30%. The GC-MS was used for the hair analysis on 51 subjects suspected to be ATSs and ketamine user. The average content of all the analytes was in the range of 1.14-12.70 ng/mg. On the basis of these results, the method was proved to be effective for identification and quantitation ofhttps://pharmacy.mahidol.ac.th/journal/_files/2023-50-1_4.pdfamphetamine-type stimulantsketaminegc-mshair |
| spellingShingle | Tran Viet Hung Ha Minh Hien Pham Quoc Chinh Pham Thi Thu Ha Nguyen Duc Nhu Nguyen Duc Tuan Development and validation of a GC-MS method for determination of amphetamine-type stimulants and ketamine in human hair Pharmaceutical Sciences Asia amphetamine-type stimulants ketamine gc-ms hair |
| title | Development and validation of a GC-MS method for determination of amphetamine-type stimulants and ketamine in human hair |
| title_full | Development and validation of a GC-MS method for determination of amphetamine-type stimulants and ketamine in human hair |
| title_fullStr | Development and validation of a GC-MS method for determination of amphetamine-type stimulants and ketamine in human hair |
| title_full_unstemmed | Development and validation of a GC-MS method for determination of amphetamine-type stimulants and ketamine in human hair |
| title_short | Development and validation of a GC-MS method for determination of amphetamine-type stimulants and ketamine in human hair |
| title_sort | development and validation of a gc ms method for determination of amphetamine type stimulants and ketamine in human hair |
| topic | amphetamine-type stimulants ketamine gc-ms hair |
| url | https://pharmacy.mahidol.ac.th/journal/_files/2023-50-1_4.pdf |
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