Immunogenicity and protective efficacy of a novel bacterium-like particle-based vaccine displaying canine distemper virus antigens in mice and dogs
ABSTRACTCanine distemper virus (CDV) poses a severe threat to both domesticated and wild animals, including multiple carnivores. With the continued expansion of its host range, there is an urgent need for the development of a safer and more effective vaccine. In this study, we developed subunit vacc...
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| Formáid: | Alt |
| Teanga: | English |
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American Society for Microbiology
2024-04-01
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| Sraith: | Microbiology Spectrum |
| Ábhair: | |
| Rochtain ar líne: | https://journals.asm.org/doi/10.1128/spectrum.03477-23 |
| _version_ | 1827297859996745728 |
|---|---|
| author | Jianzhong Wang Lina Liu Xianchun Zong Chunliu Wang Guangmei Zhu Guilian Yang Yanlong Jiang Wentao Yang Haibin Huang Chunwei Shi Yan Zeng Nan Wang Xin Cao Chunfeng Wang Na Feng |
| author_facet | Jianzhong Wang Lina Liu Xianchun Zong Chunliu Wang Guangmei Zhu Guilian Yang Yanlong Jiang Wentao Yang Haibin Huang Chunwei Shi Yan Zeng Nan Wang Xin Cao Chunfeng Wang Na Feng |
| author_sort | Jianzhong Wang |
| collection | DOAJ |
| description | ABSTRACTCanine distemper virus (CDV) poses a severe threat to both domesticated and wild animals, including multiple carnivores. With the continued expansion of its host range, there is an urgent need for the development of a safer and more effective vaccine. In this study, we developed subunit vaccines based on a bacterium-like particle (BLP) delivery platform containing BLPs-F and BLPs-H, which display the CDV F and H glycoprotein antigens, respectively, using the antigen-protein anchor fusions produced by a recombinant baculovirus insect cell expression system. The combination of BLPs-F and BLPs-H (CDV-BLPs), formulated with colloidal manganese salt [Mn jelly (MnJ)] adjuvant, triggered robust CDV-specific antibody responses and a substantial increase in the number of interferon gamma (IFN-γ)-secreting CD4+ and CD8+ T cells in mice. Dogs immunized intramuscularly with this vaccine not only produced CDV-specific IgG but also displayed elevated concentrations of IFN-γ and interleukin 6 in their serum, along with an increase of the CD3+CD4+ and CD3+CD8+ T cell subsets. Consequently, this heightened immune response provided effective protection against disease development and reduced viral shedding levels following challenge with a virulent strain. These findings suggest that this BLP-based subunit vaccine has the potential to become a novel canine distemper vaccine.IMPORTANCEMany sensitive species require a safe and effective distemper vaccine. Non-replicating vaccines are preferred. We constructed subunit particles displaying canine distemper virus (CDV) antigens based on a bacterium-like particle (BLP) delivery platform. The CDV-BLPs formulated with theMn jelly adjuvant induced robust humoral and cell-mediated immune responses to CDV in mice and dogs, thereby providing effective protection against a virulent virus challenge. This work is an important step in developing a CDV subunit vaccine. |
| first_indexed | 2024-04-24T15:03:37Z |
| format | Article |
| id | doaj.art-d73ce3a0f0a34d7788d8b2e11f71bd51 |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-04-24T15:03:37Z |
| publishDate | 2024-04-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-d73ce3a0f0a34d7788d8b2e11f71bd512024-04-02T14:16:18ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972024-04-0112410.1128/spectrum.03477-23Immunogenicity and protective efficacy of a novel bacterium-like particle-based vaccine displaying canine distemper virus antigens in mice and dogsJianzhong Wang0Lina Liu1Xianchun Zong2Chunliu Wang3Guangmei Zhu4Guilian Yang5Yanlong Jiang6Wentao Yang7Haibin Huang8Chunwei Shi9Yan Zeng10Nan Wang11Xin Cao12Chunfeng Wang13Na Feng14College of Veterinary Medicine, Jilin Agricultural University, Changchun, ChinaChangchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, ChinaCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, ChinaChangchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, ChinaABSTRACTCanine distemper virus (CDV) poses a severe threat to both domesticated and wild animals, including multiple carnivores. With the continued expansion of its host range, there is an urgent need for the development of a safer and more effective vaccine. In this study, we developed subunit vaccines based on a bacterium-like particle (BLP) delivery platform containing BLPs-F and BLPs-H, which display the CDV F and H glycoprotein antigens, respectively, using the antigen-protein anchor fusions produced by a recombinant baculovirus insect cell expression system. The combination of BLPs-F and BLPs-H (CDV-BLPs), formulated with colloidal manganese salt [Mn jelly (MnJ)] adjuvant, triggered robust CDV-specific antibody responses and a substantial increase in the number of interferon gamma (IFN-γ)-secreting CD4+ and CD8+ T cells in mice. Dogs immunized intramuscularly with this vaccine not only produced CDV-specific IgG but also displayed elevated concentrations of IFN-γ and interleukin 6 in their serum, along with an increase of the CD3+CD4+ and CD3+CD8+ T cell subsets. Consequently, this heightened immune response provided effective protection against disease development and reduced viral shedding levels following challenge with a virulent strain. These findings suggest that this BLP-based subunit vaccine has the potential to become a novel canine distemper vaccine.IMPORTANCEMany sensitive species require a safe and effective distemper vaccine. Non-replicating vaccines are preferred. We constructed subunit particles displaying canine distemper virus (CDV) antigens based on a bacterium-like particle (BLP) delivery platform. The CDV-BLPs formulated with theMn jelly adjuvant induced robust humoral and cell-mediated immune responses to CDV in mice and dogs, thereby providing effective protection against a virulent virus challenge. This work is an important step in developing a CDV subunit vaccine.https://journals.asm.org/doi/10.1128/spectrum.03477-23bacterium-like particlescanine distemper virussubunit vaccinesurface display technologyimmune response |
| spellingShingle | Jianzhong Wang Lina Liu Xianchun Zong Chunliu Wang Guangmei Zhu Guilian Yang Yanlong Jiang Wentao Yang Haibin Huang Chunwei Shi Yan Zeng Nan Wang Xin Cao Chunfeng Wang Na Feng Immunogenicity and protective efficacy of a novel bacterium-like particle-based vaccine displaying canine distemper virus antigens in mice and dogs Microbiology Spectrum bacterium-like particles canine distemper virus subunit vaccine surface display technology immune response |
| title | Immunogenicity and protective efficacy of a novel bacterium-like particle-based vaccine displaying canine distemper virus antigens in mice and dogs |
| title_full | Immunogenicity and protective efficacy of a novel bacterium-like particle-based vaccine displaying canine distemper virus antigens in mice and dogs |
| title_fullStr | Immunogenicity and protective efficacy of a novel bacterium-like particle-based vaccine displaying canine distemper virus antigens in mice and dogs |
| title_full_unstemmed | Immunogenicity and protective efficacy of a novel bacterium-like particle-based vaccine displaying canine distemper virus antigens in mice and dogs |
| title_short | Immunogenicity and protective efficacy of a novel bacterium-like particle-based vaccine displaying canine distemper virus antigens in mice and dogs |
| title_sort | immunogenicity and protective efficacy of a novel bacterium like particle based vaccine displaying canine distemper virus antigens in mice and dogs |
| topic | bacterium-like particles canine distemper virus subunit vaccine surface display technology immune response |
| url | https://journals.asm.org/doi/10.1128/spectrum.03477-23 |
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