Iron Bioavailability in the Extracellular Environment Is More Relevant Than the Intracellular One in Viability and Gene Expression: A Lesson from Oligodendroglioma Cells
Oligodendroglioma (OG) is a brain tumor that contributes to <1% of brain tumor diagnoses in the pediatric population. Unfortunately, pediatric OG remains without definitive molecular characteristics to aid in diagnosis, and little is known about the tumor microenvironment. Tumor cells’ metabolism...
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MDPI AG
2023-10-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/11/2940 |
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| author | Stefania Braidotti Debora Curci Daniele Zampieri Cesare Covino Davide Zanon Natalia Maximova Roberto Sala |
| author_facet | Stefania Braidotti Debora Curci Daniele Zampieri Cesare Covino Davide Zanon Natalia Maximova Roberto Sala |
| author_sort | Stefania Braidotti |
| collection | DOAJ |
| description | Oligodendroglioma (OG) is a brain tumor that contributes to <1% of brain tumor diagnoses in the pediatric population. Unfortunately, pediatric OG remains without definitive molecular characteristics to aid in diagnosis, and little is known about the tumor microenvironment. Tumor cells’ metabolism and proliferation rate are generally higher than those of healthy cells, so their iron demand is also significantly higher. This consideration underlines the great importance of iron for tumor development and progression. In this context, this study aims to evaluate the effect of iron in a cellular in vitro model of human oligodendroglioma brain tumor. Cell morphology, the effect of siderotic medium on cell growth, iron uptake, and the expression of iron-metabolism-related genes were evaluated via optic microscopy, ICP-MS, confocal microscopy, and real-time PCR, respectively. This study underlines the great importance of iron for tumor development and progression and also the possibility of reducing the available iron concentration to determine an antiproliferative effect on OG. Therefore, every attempt can be promising to defeat OG for which there are currently no long-term curative therapies. |
| first_indexed | 2024-03-09T17:01:00Z |
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| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T17:01:00Z |
| publishDate | 2023-10-01 |
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| series | Biomedicines |
| spelling | doaj.art-d741989bc1c84bffacde736dece461af2023-11-24T14:30:50ZengMDPI AGBiomedicines2227-90592023-10-011111294010.3390/biomedicines11112940Iron Bioavailability in the Extracellular Environment Is More Relevant Than the Intracellular One in Viability and Gene Expression: A Lesson from Oligodendroglioma CellsStefania Braidotti0Debora Curci1Daniele Zampieri2Cesare Covino3Davide Zanon4Natalia Maximova5Roberto Sala6Department of Pediatrics, Institute for Maternal and Child Health-IRCCS Burlo Garofolo, 34137 Trieste, ItalyAdvanced Translational Diagnostic Laboratory, Institute for Maternal and Child Health-IRCCS Burlo Garofolo, 34137 Trieste, ItalyDepartment of Chemical and Pharmaceutical Sciences, University of Trieste, 34127 Trieste, ItalyAdvanced Light and Electron Microscopy Imaging Centre (ALEMBIC), IRCCS San Raffaele Scientific Institute, 20132 Milan, ItalyPharmacy and Clinical Pharmacology Department, Institute for Maternal and Child Health-IRCCS Burlo Garofolo, 34137 Trieste, ItalyDepartment of Pediatrics, Institute for Maternal and Child Health-IRCCS Burlo Garofolo, 34137 Trieste, ItalyDepartment of Medicine and Surgery, University of Parma, 43121 Parma, ItalyOligodendroglioma (OG) is a brain tumor that contributes to <1% of brain tumor diagnoses in the pediatric population. Unfortunately, pediatric OG remains without definitive molecular characteristics to aid in diagnosis, and little is known about the tumor microenvironment. Tumor cells’ metabolism and proliferation rate are generally higher than those of healthy cells, so their iron demand is also significantly higher. This consideration underlines the great importance of iron for tumor development and progression. In this context, this study aims to evaluate the effect of iron in a cellular in vitro model of human oligodendroglioma brain tumor. Cell morphology, the effect of siderotic medium on cell growth, iron uptake, and the expression of iron-metabolism-related genes were evaluated via optic microscopy, ICP-MS, confocal microscopy, and real-time PCR, respectively. This study underlines the great importance of iron for tumor development and progression and also the possibility of reducing the available iron concentration to determine an antiproliferative effect on OG. Therefore, every attempt can be promising to defeat OG for which there are currently no long-term curative therapies.https://www.mdpi.com/2227-9059/11/11/2940oligodendrogliomaironpediatricbrain tumors |
| spellingShingle | Stefania Braidotti Debora Curci Daniele Zampieri Cesare Covino Davide Zanon Natalia Maximova Roberto Sala Iron Bioavailability in the Extracellular Environment Is More Relevant Than the Intracellular One in Viability and Gene Expression: A Lesson from Oligodendroglioma Cells Biomedicines oligodendroglioma iron pediatric brain tumors |
| title | Iron Bioavailability in the Extracellular Environment Is More Relevant Than the Intracellular One in Viability and Gene Expression: A Lesson from Oligodendroglioma Cells |
| title_full | Iron Bioavailability in the Extracellular Environment Is More Relevant Than the Intracellular One in Viability and Gene Expression: A Lesson from Oligodendroglioma Cells |
| title_fullStr | Iron Bioavailability in the Extracellular Environment Is More Relevant Than the Intracellular One in Viability and Gene Expression: A Lesson from Oligodendroglioma Cells |
| title_full_unstemmed | Iron Bioavailability in the Extracellular Environment Is More Relevant Than the Intracellular One in Viability and Gene Expression: A Lesson from Oligodendroglioma Cells |
| title_short | Iron Bioavailability in the Extracellular Environment Is More Relevant Than the Intracellular One in Viability and Gene Expression: A Lesson from Oligodendroglioma Cells |
| title_sort | iron bioavailability in the extracellular environment is more relevant than the intracellular one in viability and gene expression a lesson from oligodendroglioma cells |
| topic | oligodendroglioma iron pediatric brain tumors |
| url | https://www.mdpi.com/2227-9059/11/11/2940 |
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