Nylon wool column: a tool for obtaining monokine-rich eluates in the absence of serum
Diverse conditions for stimulating human mononuclear cells to release thymocyte costimulatory factors were tested for their contribution to the generation of supernatants high titers of these monokines. Activity titers increased with LPS concentration, reaching a plateau between 1 and 10 microng/ml....
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
1990-06-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000200003 |
| _version_ | 1797718112727990272 |
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| author | S. R. Dalmau C. S. Freitas |
| author_facet | S. R. Dalmau C. S. Freitas |
| author_sort | S. R. Dalmau |
| collection | DOAJ |
| description | Diverse conditions for stimulating human mononuclear cells to release thymocyte costimulatory factors were tested for their contribution to the generation of supernatants high titers of these monokines. Activity titers increased with LPS concentration, reaching a plateau between 1 and 10 microng/ml. Indomethacin did not modify the monokine, but the assay for thymocyte costimulatory activity was substantially affected by inhibitory substances produced by the monocytes in the absence of indomethacin. The use of nylon wool columns to trap the cells was shown to be effective in raising cellular densities without decreasing activity titers. As result, the yield per cell could be maintained even in the absence of serum, an important step toward the goal of purifiying bioactive from crude broths. |
| first_indexed | 2024-03-12T08:46:15Z |
| format | Article |
| id | doaj.art-d74246c7b5ed437d85716885d014ee57 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:46:15Z |
| publishDate | 1990-06-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-d74246c7b5ed437d85716885d014ee572023-09-02T16:26:12ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80601990-06-0185215316210.1590/S0074-02761990000200003Nylon wool column: a tool for obtaining monokine-rich eluates in the absence of serumS. R. DalmauC. S. FreitasDiverse conditions for stimulating human mononuclear cells to release thymocyte costimulatory factors were tested for their contribution to the generation of supernatants high titers of these monokines. Activity titers increased with LPS concentration, reaching a plateau between 1 and 10 microng/ml. Indomethacin did not modify the monokine, but the assay for thymocyte costimulatory activity was substantially affected by inhibitory substances produced by the monocytes in the absence of indomethacin. The use of nylon wool columns to trap the cells was shown to be effective in raising cellular densities without decreasing activity titers. As result, the yield per cell could be maintained even in the absence of serum, an important step toward the goal of purifiying bioactive from crude broths.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000200003nylon wool columnshuman monokine productionthymocyte costimulatory assayLPSperipheral blood monocytes |
| spellingShingle | S. R. Dalmau C. S. Freitas Nylon wool column: a tool for obtaining monokine-rich eluates in the absence of serum Memorias do Instituto Oswaldo Cruz nylon wool columns human monokine production thymocyte costimulatory assay LPS peripheral blood monocytes |
| title | Nylon wool column: a tool for obtaining monokine-rich eluates in the absence of serum |
| title_full | Nylon wool column: a tool for obtaining monokine-rich eluates in the absence of serum |
| title_fullStr | Nylon wool column: a tool for obtaining monokine-rich eluates in the absence of serum |
| title_full_unstemmed | Nylon wool column: a tool for obtaining monokine-rich eluates in the absence of serum |
| title_short | Nylon wool column: a tool for obtaining monokine-rich eluates in the absence of serum |
| title_sort | nylon wool column a tool for obtaining monokine rich eluates in the absence of serum |
| topic | nylon wool columns human monokine production thymocyte costimulatory assay LPS peripheral blood monocytes |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000200003 |
| work_keys_str_mv | AT srdalmau nylonwoolcolumnatoolforobtainingmonokinericheluatesintheabsenceofserum AT csfreitas nylonwoolcolumnatoolforobtainingmonokinericheluatesintheabsenceofserum |