Protective effect of chrysophanol on neural degeneration caused by glaucoma and its mechanism
AIM:To investigate the effect and mechanism of chrysophanol for rat model with glaucoma.<p>METHODS:The glaucoma rat models were made by cauterization of three episcleral veins. Then the glaucoma rats were divided into three groups. Group 1 was the untreated intraocular hypertension group. Grou...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Press of International Journal of Ophthalmology (IJO PRESS)
2016-01-01
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| Series: | Guoji Yanke Zazhi |
| Subjects: | |
| Online Access: | http://ies.ijo.cn/cn_publish/2016/1/201601008.pdf |
| Summary: | AIM:To investigate the effect and mechanism of chrysophanol for rat model with glaucoma.<p>METHODS:The glaucoma rat models were made by cauterization of three episcleral veins. Then the glaucoma rats were divided into three groups. Group 1 was the untreated intraocular hypertension group. Group 2 was the low dose of chrysophanol group(25mg/kg). Group 3 was the high dose of chrysophanol group(50mg/kg), 15 rats in each group. The right eyes were the experiment eyes while the left were the control ones. After 6wk treatment, the mRNA and protein of protein kinase-like endoplasmic reticulum kinase(PERK)and Rho kinase 1(ROCK-1)were determined in the retina. <p>RESULTS:The chrysophanol reduced intraocular pressure(IOP)of experiment eyes, which was significantly lower than that of control eyes(<i>P</i><0.01). Compared with the normal group, the p-PERK protein increased significantly in the retina of glaucoma model group and chrysophanol increased the lever of p-PERK protein. The ROCK-1 protein level increased significantly in glaucoma group, it all decreased in these treatment groups, and it decreased significantly in high dose treatment group. Detected by TR-PCR, chrysophanol also could activate the mRNA of PERK and inhibited the mRNA expression of ROCK-1 in a rat model of glaucoma.<p>CONCLUSION:These results suggest that chrysophanol can reduce the IOP through the phosphorylation of PERK protein to regulate the PERK/ROCK signaling in glaucoma rat model. |
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| ISSN: | 1672-5123 1672-5123 |