Chemical Composition and Biological Activities of <i>Centranthus longiflorus</i> Stems Extracts Recovered Using <i>Ired-Irrad</i><sup>®</sup>, an Innovative Infrared Technology, Compared to Water Bath and Ultrasound
Extraction of polyphenols from <i>Centranthus longiflorus</i> stems was conducted using ultrasound and infrared <i>Ired-Irrad</i><sup>®</sup> techniques, and compared to the conventional water bath method. Response surface methodology was used to analyse the effec...
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MDPI AG
2023-05-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/13/6/1288 |
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| author | Mariam Hammoud Hiba N. Rajha Ali Chokr Carl Safi Lambertus A. M. van den Broek Gijs van Erven Richard G. Maroun Espérance Debs Hassan Rammal Nicolas Louka |
| author_facet | Mariam Hammoud Hiba N. Rajha Ali Chokr Carl Safi Lambertus A. M. van den Broek Gijs van Erven Richard G. Maroun Espérance Debs Hassan Rammal Nicolas Louka |
| author_sort | Mariam Hammoud |
| collection | DOAJ |
| description | Extraction of polyphenols from <i>Centranthus longiflorus</i> stems was conducted using ultrasound and infrared <i>Ired-Irrad</i><sup>®</sup> techniques, and compared to the conventional water bath method. Response surface methodology was used to analyse the effect of time, temperature, and ethanol percentage, as well as to optimize the three extraction methods. The highest phenolic content (81 mg GAE/g DM) and antioxidant activity (76% DPPH inhibition) were recorded with the <i>Ired-Irrad</i><sup>®</sup> extract obtained under the optimal conditions: 55 °C, 127 min, 48% (<i>v</i>/<i>v</i>) ethanol. Biological activities (antioxidant, antibacterial and antibiofilm) of the three extracts were assessed. All <i>C</i>. <i>longiflorus</i> stems extracts showed limited antibacterial effects regardless of the extraction method (MIC = 50 mg/mL), whereas <i>Ired-Irrad</i><sup>®</sup> extract exhibited the highest biofilm eradication and prevention capacities (93% against <i>Escherichia coli</i> and 97% against <i>Staphylococcus epidermidis</i>, respectively). This bioactivity is likely related to abundant caffeoylquinic acid and quercetin rutinoside, as identified by RP-UHPLC-PDA-MS analysis. The results obtained further promote the effectiveness of <i>Ired-Irrad</i><sup>®</sup> as a highly flexible and cost-efficient extraction technique. |
| first_indexed | 2024-03-11T02:14:53Z |
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| language | English |
| last_indexed | 2024-03-11T02:14:53Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
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| series | Life |
| spelling | doaj.art-d74dfc547b1949e1aefec217493761512023-11-18T11:17:14ZengMDPI AGLife2075-17292023-05-01136128810.3390/life13061288Chemical Composition and Biological Activities of <i>Centranthus longiflorus</i> Stems Extracts Recovered Using <i>Ired-Irrad</i><sup>®</sup>, an Innovative Infrared Technology, Compared to Water Bath and UltrasoundMariam Hammoud0Hiba N. Rajha1Ali Chokr2Carl Safi3Lambertus A. M. van den Broek4Gijs van Erven5Richard G. Maroun6Espérance Debs7Hassan Rammal8Nicolas Louka9Centre d’Analyses et de Recherche, Unité de Recherche Technologies et Valorisation Agro-Alimentaire, Faculté des Sciences, Université Saint-Joseph de Beyrouth, CST-Mar Roukos-Dekwaneh, Riad El Solh, P.O. Box 1514, Beirut 1107 2050, LebanonCentre d’Analyses et de Recherche, Unité de Recherche Technologies et Valorisation Agro-Alimentaire, Faculté des Sciences, Université Saint-Joseph de Beyrouth, CST-Mar Roukos-Dekwaneh, Riad El Solh, P.O. Box 1514, Beirut 1107 2050, LebanonResearch Laboratory of Microbiology (RLM), Department of Life and Earth Sciences, Faculty of Sciences I, Lebanese University, Hadath Campus, P.O. Box 5, Beirut 1683, LebanonWageningen Food & Biobased Research, P.O. Box 17, 6700 AA Wageningen, The NetherlandsWageningen Food & Biobased Research, P.O. Box 17, 6700 AA Wageningen, The NetherlandsWageningen Food & Biobased Research, P.O. Box 17, 6700 AA Wageningen, The NetherlandsCentre d’Analyses et de Recherche, Unité de Recherche Technologies et Valorisation Agro-Alimentaire, Faculté des Sciences, Université Saint-Joseph de Beyrouth, CST-Mar Roukos-Dekwaneh, Riad El Solh, P.O. Box 1514, Beirut 1107 2050, LebanonDepartment of Biology, Faculty of Arts and Sciences, University of Balamand, P.O. Box 100, Tripoli 1300, LebanonResearch Laboratory of Microbiology (RLM), Department of Life and Earth Sciences, Faculty of Sciences I, Lebanese University, Hadath Campus, P.O. Box 5, Beirut 1683, LebanonCentre d’Analyses et de Recherche, Unité de Recherche Technologies et Valorisation Agro-Alimentaire, Faculté des Sciences, Université Saint-Joseph de Beyrouth, CST-Mar Roukos-Dekwaneh, Riad El Solh, P.O. Box 1514, Beirut 1107 2050, LebanonExtraction of polyphenols from <i>Centranthus longiflorus</i> stems was conducted using ultrasound and infrared <i>Ired-Irrad</i><sup>®</sup> techniques, and compared to the conventional water bath method. Response surface methodology was used to analyse the effect of time, temperature, and ethanol percentage, as well as to optimize the three extraction methods. The highest phenolic content (81 mg GAE/g DM) and antioxidant activity (76% DPPH inhibition) were recorded with the <i>Ired-Irrad</i><sup>®</sup> extract obtained under the optimal conditions: 55 °C, 127 min, 48% (<i>v</i>/<i>v</i>) ethanol. Biological activities (antioxidant, antibacterial and antibiofilm) of the three extracts were assessed. All <i>C</i>. <i>longiflorus</i> stems extracts showed limited antibacterial effects regardless of the extraction method (MIC = 50 mg/mL), whereas <i>Ired-Irrad</i><sup>®</sup> extract exhibited the highest biofilm eradication and prevention capacities (93% against <i>Escherichia coli</i> and 97% against <i>Staphylococcus epidermidis</i>, respectively). This bioactivity is likely related to abundant caffeoylquinic acid and quercetin rutinoside, as identified by RP-UHPLC-PDA-MS analysis. The results obtained further promote the effectiveness of <i>Ired-Irrad</i><sup>®</sup> as a highly flexible and cost-efficient extraction technique.https://www.mdpi.com/2075-1729/13/6/1288<i>Centranthus longiflorus</i>phenolic compounds<i>Ired-Irrad</i><sup>®</sup>ultrasoundantibacterial activity |
| spellingShingle | Mariam Hammoud Hiba N. Rajha Ali Chokr Carl Safi Lambertus A. M. van den Broek Gijs van Erven Richard G. Maroun Espérance Debs Hassan Rammal Nicolas Louka Chemical Composition and Biological Activities of <i>Centranthus longiflorus</i> Stems Extracts Recovered Using <i>Ired-Irrad</i><sup>®</sup>, an Innovative Infrared Technology, Compared to Water Bath and Ultrasound Life <i>Centranthus longiflorus</i> phenolic compounds <i>Ired-Irrad</i><sup>®</sup> ultrasound antibacterial activity |
| title | Chemical Composition and Biological Activities of <i>Centranthus longiflorus</i> Stems Extracts Recovered Using <i>Ired-Irrad</i><sup>®</sup>, an Innovative Infrared Technology, Compared to Water Bath and Ultrasound |
| title_full | Chemical Composition and Biological Activities of <i>Centranthus longiflorus</i> Stems Extracts Recovered Using <i>Ired-Irrad</i><sup>®</sup>, an Innovative Infrared Technology, Compared to Water Bath and Ultrasound |
| title_fullStr | Chemical Composition and Biological Activities of <i>Centranthus longiflorus</i> Stems Extracts Recovered Using <i>Ired-Irrad</i><sup>®</sup>, an Innovative Infrared Technology, Compared to Water Bath and Ultrasound |
| title_full_unstemmed | Chemical Composition and Biological Activities of <i>Centranthus longiflorus</i> Stems Extracts Recovered Using <i>Ired-Irrad</i><sup>®</sup>, an Innovative Infrared Technology, Compared to Water Bath and Ultrasound |
| title_short | Chemical Composition and Biological Activities of <i>Centranthus longiflorus</i> Stems Extracts Recovered Using <i>Ired-Irrad</i><sup>®</sup>, an Innovative Infrared Technology, Compared to Water Bath and Ultrasound |
| title_sort | chemical composition and biological activities of i centranthus longiflorus i stems extracts recovered using i ired irrad i sup r sup an innovative infrared technology compared to water bath and ultrasound |
| topic | <i>Centranthus longiflorus</i> phenolic compounds <i>Ired-Irrad</i><sup>®</sup> ultrasound antibacterial activity |
| url | https://www.mdpi.com/2075-1729/13/6/1288 |
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