Bayesian estimation of Pseudomonas aeruginosa viscoelastic properties based on creep responses of wild type, rugose, and mucoid variant biofilms
Pseudomonas aeruginosa biofilms are relevant for a variety of disease settings, including pulmonary infections in people with cystic fibrosis. Biofilms are initiated by individual bacteria that undergo a phenotypic switch and produce an extracellular polymeric slime (EPS). However, the viscoelastic...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Biofilm |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590207523000308 |
| _version_ | 1797801005504528384 |
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| author | Mohammad Nooranidoost N.G. Cogan Paul Stoodley Erin S. Gloag M. Yousuff Hussaini |
| author_facet | Mohammad Nooranidoost N.G. Cogan Paul Stoodley Erin S. Gloag M. Yousuff Hussaini |
| author_sort | Mohammad Nooranidoost |
| collection | DOAJ |
| description | Pseudomonas aeruginosa biofilms are relevant for a variety of disease settings, including pulmonary infections in people with cystic fibrosis. Biofilms are initiated by individual bacteria that undergo a phenotypic switch and produce an extracellular polymeric slime (EPS). However, the viscoelastic characteristics of biofilms at different stages of formation and the contributions of different EPS constituents have not been fully explored. For this purpose, we develop and parameterize a mathematical model to study the rheological behavior of three biofilms — P. aeruginosa wild type PAO1, isogenic rugose small colony variant (RSCV), and mucoid variant biofilms against a range of experimental data. Using Bayesian inference to estimate these viscoelastic properties, we quantify the rheological characteristics of the biofilm EPS. We employ a Monte Carlo Markov Chain algorithm to estimate these properties of P. aeruginosa variant biofilms in comparison to those of wild type. This information helps us understand the rheological behavior of biofilms at different stages of their development. The mechanical properties of wild type biofilms change significantly over time and are more sensitive to small changes in their composition than the other two mutants. |
| first_indexed | 2024-03-13T04:43:51Z |
| format | Article |
| id | doaj.art-d7515198c2a04e80b72cb356b768c88f |
| institution | Directory Open Access Journal |
| issn | 2590-2075 |
| language | English |
| last_indexed | 2024-03-13T04:43:51Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biofilm |
| spelling | doaj.art-d7515198c2a04e80b72cb356b768c88f2023-06-19T04:29:37ZengElsevierBiofilm2590-20752023-12-015100133Bayesian estimation of Pseudomonas aeruginosa viscoelastic properties based on creep responses of wild type, rugose, and mucoid variant biofilmsMohammad Nooranidoost0N.G. Cogan1Paul Stoodley2Erin S. Gloag3M. Yousuff Hussaini4Department of Mathematics, Florida State University, Tallahassee, FL, USA; Corresponding author.Department of Mathematics, Florida State University, Tallahassee, FL, USADepartment of Microbial Infection and Immunity, The Ohio State University, Columbus, OH, USA; Department of Orthopaedics, The Ohio State University, Columbus, OH, USA; National Centre for Advanced Tribology at Southampton (nCATS), National Biofilm Innovation Centre (NBIC), Department of Mechanical Engineering, University of Southampton, UK; Corresponding author. National Centre for Advanced Tribology at Southampton (nCATS), Department Mechanical Engineering, University of Southampton, SO17 1BJ, UK.Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Tech, Blacksburg, VA, USADepartment of Mathematics, Florida State University, Tallahassee, FL, USAPseudomonas aeruginosa biofilms are relevant for a variety of disease settings, including pulmonary infections in people with cystic fibrosis. Biofilms are initiated by individual bacteria that undergo a phenotypic switch and produce an extracellular polymeric slime (EPS). However, the viscoelastic characteristics of biofilms at different stages of formation and the contributions of different EPS constituents have not been fully explored. For this purpose, we develop and parameterize a mathematical model to study the rheological behavior of three biofilms — P. aeruginosa wild type PAO1, isogenic rugose small colony variant (RSCV), and mucoid variant biofilms against a range of experimental data. Using Bayesian inference to estimate these viscoelastic properties, we quantify the rheological characteristics of the biofilm EPS. We employ a Monte Carlo Markov Chain algorithm to estimate these properties of P. aeruginosa variant biofilms in comparison to those of wild type. This information helps us understand the rheological behavior of biofilms at different stages of their development. The mechanical properties of wild type biofilms change significantly over time and are more sensitive to small changes in their composition than the other two mutants.http://www.sciencedirect.com/science/article/pii/S2590207523000308BiofilmViscoelasticityBiomechanicsExtracellular polymeric slimeBayesian estimation |
| spellingShingle | Mohammad Nooranidoost N.G. Cogan Paul Stoodley Erin S. Gloag M. Yousuff Hussaini Bayesian estimation of Pseudomonas aeruginosa viscoelastic properties based on creep responses of wild type, rugose, and mucoid variant biofilms Biofilm Biofilm Viscoelasticity Biomechanics Extracellular polymeric slime Bayesian estimation |
| title | Bayesian estimation of Pseudomonas aeruginosa viscoelastic properties based on creep responses of wild type, rugose, and mucoid variant biofilms |
| title_full | Bayesian estimation of Pseudomonas aeruginosa viscoelastic properties based on creep responses of wild type, rugose, and mucoid variant biofilms |
| title_fullStr | Bayesian estimation of Pseudomonas aeruginosa viscoelastic properties based on creep responses of wild type, rugose, and mucoid variant biofilms |
| title_full_unstemmed | Bayesian estimation of Pseudomonas aeruginosa viscoelastic properties based on creep responses of wild type, rugose, and mucoid variant biofilms |
| title_short | Bayesian estimation of Pseudomonas aeruginosa viscoelastic properties based on creep responses of wild type, rugose, and mucoid variant biofilms |
| title_sort | bayesian estimation of pseudomonas aeruginosa viscoelastic properties based on creep responses of wild type rugose and mucoid variant biofilms |
| topic | Biofilm Viscoelasticity Biomechanics Extracellular polymeric slime Bayesian estimation |
| url | http://www.sciencedirect.com/science/article/pii/S2590207523000308 |
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