Extremely Halophilic Biohydrogen Producing Microbial Communities from High-Salinity Soil and Salt Evaporation Pond
Extreme halophiles offer the advantage to save on the costs of sterilization and water for biohydrogen production from lignocellulosic waste after the pretreatment process with their ability to withstand extreme salt concentrations. This study identifies the dominant hydrogen-producing genera and sp...
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| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Fuels |
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| Online Access: | https://www.mdpi.com/2673-3994/2/2/14 |
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| author | Dyah Asri Handayani Taroepratjeka Tsuyoshi Imai Prapaipid Chairattanamanokorn Alissara Reungsang |
| author_facet | Dyah Asri Handayani Taroepratjeka Tsuyoshi Imai Prapaipid Chairattanamanokorn Alissara Reungsang |
| author_sort | Dyah Asri Handayani Taroepratjeka |
| collection | DOAJ |
| description | Extreme halophiles offer the advantage to save on the costs of sterilization and water for biohydrogen production from lignocellulosic waste after the pretreatment process with their ability to withstand extreme salt concentrations. This study identifies the dominant hydrogen-producing genera and species among the acclimatized, extremely halotolerant microbial communities taken from two salt-damaged soil locations in Khon Kaen and one location from the salt evaporation pond in Samut Sakhon, Thailand. The microbial communities’ V3–V4 regions of 16srRNA were analyzed using high-throughput amplicon sequencing. A total of 345 operational taxonomic units were obtained and the high-throughput sequencing confirmed that <i>Firmicutes</i> was the dominant phyla of the three communities. <i>Halanaerobium fermentans</i> and <i>Halanaerobacter lacunarum</i> were the dominant hydrogen-producing species of the communities. Spatial proximity was not found to be a determining factor for similarities between these extremely halophilic microbial communities. Through the study of the microbial communities, strategies can be developed to increase biohydrogen molar yield. |
| first_indexed | 2024-03-10T10:31:41Z |
| format | Article |
| id | doaj.art-d756b2a0bec04fe39f79484558918f13 |
| institution | Directory Open Access Journal |
| issn | 2673-3994 |
| language | English |
| last_indexed | 2024-03-10T10:31:41Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fuels |
| spelling | doaj.art-d756b2a0bec04fe39f79484558918f132023-11-21T23:37:05ZengMDPI AGFuels2673-39942021-06-012224125210.3390/fuels2020014Extremely Halophilic Biohydrogen Producing Microbial Communities from High-Salinity Soil and Salt Evaporation PondDyah Asri Handayani Taroepratjeka0Tsuyoshi Imai1Prapaipid Chairattanamanokorn2Alissara Reungsang3Department of Environmental Engineering, Institut Teknologi Nasional Bandung, Jawa Barat 40124, IndonesiaGraduate School of Sciences and Technology for Innovation, Yamaguchi University, Yamaguchi 755-8611, JapanDepartment of Environmental Engineering and Management, Faculty of Environment, Kasetsart University, Bangkok 10900, ThailandDepartment of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, ThailandExtreme halophiles offer the advantage to save on the costs of sterilization and water for biohydrogen production from lignocellulosic waste after the pretreatment process with their ability to withstand extreme salt concentrations. This study identifies the dominant hydrogen-producing genera and species among the acclimatized, extremely halotolerant microbial communities taken from two salt-damaged soil locations in Khon Kaen and one location from the salt evaporation pond in Samut Sakhon, Thailand. The microbial communities’ V3–V4 regions of 16srRNA were analyzed using high-throughput amplicon sequencing. A total of 345 operational taxonomic units were obtained and the high-throughput sequencing confirmed that <i>Firmicutes</i> was the dominant phyla of the three communities. <i>Halanaerobium fermentans</i> and <i>Halanaerobacter lacunarum</i> were the dominant hydrogen-producing species of the communities. Spatial proximity was not found to be a determining factor for similarities between these extremely halophilic microbial communities. Through the study of the microbial communities, strategies can be developed to increase biohydrogen molar yield.https://www.mdpi.com/2673-3994/2/2/14high-throughput sequencing<i>Halanaerobium fermentans</i>extreme halophilesbiohydrogen |
| spellingShingle | Dyah Asri Handayani Taroepratjeka Tsuyoshi Imai Prapaipid Chairattanamanokorn Alissara Reungsang Extremely Halophilic Biohydrogen Producing Microbial Communities from High-Salinity Soil and Salt Evaporation Pond Fuels high-throughput sequencing <i>Halanaerobium fermentans</i> extreme halophiles biohydrogen |
| title | Extremely Halophilic Biohydrogen Producing Microbial Communities from High-Salinity Soil and Salt Evaporation Pond |
| title_full | Extremely Halophilic Biohydrogen Producing Microbial Communities from High-Salinity Soil and Salt Evaporation Pond |
| title_fullStr | Extremely Halophilic Biohydrogen Producing Microbial Communities from High-Salinity Soil and Salt Evaporation Pond |
| title_full_unstemmed | Extremely Halophilic Biohydrogen Producing Microbial Communities from High-Salinity Soil and Salt Evaporation Pond |
| title_short | Extremely Halophilic Biohydrogen Producing Microbial Communities from High-Salinity Soil and Salt Evaporation Pond |
| title_sort | extremely halophilic biohydrogen producing microbial communities from high salinity soil and salt evaporation pond |
| topic | high-throughput sequencing <i>Halanaerobium fermentans</i> extreme halophiles biohydrogen |
| url | https://www.mdpi.com/2673-3994/2/2/14 |
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