Tools of the trade: plasmid repositories and standardized plasmid manipulation for molecular and synthetic biology
Summary Plasmids are extrachromosomal genetic elements capable of autonomous replication within a host cell. They play a key role in bacterial ecology and evolution, facilitating the mobilization of accessory genes by horizontal gene transfer. Crucially, plasmids also serve as valuable tools in mode...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-05-01
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| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.14012 |
| _version_ | 1828499376827269120 |
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| author | Stephen K. Dolan Miguel A. Matilla |
| author_facet | Stephen K. Dolan Miguel A. Matilla |
| author_sort | Stephen K. Dolan |
| collection | DOAJ |
| description | Summary Plasmids are extrachromosomal genetic elements capable of autonomous replication within a host cell. They play a key role in bacterial ecology and evolution, facilitating the mobilization of accessory genes by horizontal gene transfer. Crucially, plasmids also serve as valuable tools in modern molecular biology. Here, we highlight recent articles aimed at implementing standardized plasmid assembly techniques and plasmid repositories to promote open science as well as to improve experimental reproducibility across laboratories. Research focused on assisting these fundamental aims is a further step towards improving standardization in molecular and synthetic biology. |
| first_indexed | 2024-12-11T13:23:33Z |
| format | Article |
| id | doaj.art-d757f3a6d1f7483fa4a3aa3b9083ba09 |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-12-11T13:23:33Z |
| publishDate | 2022-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-d757f3a6d1f7483fa4a3aa3b9083ba092022-12-22T01:05:41ZengWileyMicrobial Biotechnology1751-79152022-05-011551318132010.1111/1751-7915.14012Tools of the trade: plasmid repositories and standardized plasmid manipulation for molecular and synthetic biologyStephen K. Dolan0Miguel A. Matilla1School of Biological Sciences Georgia Institute of Technology Atlanta GA 30332 USADepartment of Biotechnology and Environmental Protection Estación Experimental del Zaidín Consejo Superior de Investigaciones Científicas Prof. Albareda 1 Granada 18008 SpainSummary Plasmids are extrachromosomal genetic elements capable of autonomous replication within a host cell. They play a key role in bacterial ecology and evolution, facilitating the mobilization of accessory genes by horizontal gene transfer. Crucially, plasmids also serve as valuable tools in modern molecular biology. Here, we highlight recent articles aimed at implementing standardized plasmid assembly techniques and plasmid repositories to promote open science as well as to improve experimental reproducibility across laboratories. Research focused on assisting these fundamental aims is a further step towards improving standardization in molecular and synthetic biology.https://doi.org/10.1111/1751-7915.14012 |
| spellingShingle | Stephen K. Dolan Miguel A. Matilla Tools of the trade: plasmid repositories and standardized plasmid manipulation for molecular and synthetic biology Microbial Biotechnology |
| title | Tools of the trade: plasmid repositories and standardized plasmid manipulation for molecular and synthetic biology |
| title_full | Tools of the trade: plasmid repositories and standardized plasmid manipulation for molecular and synthetic biology |
| title_fullStr | Tools of the trade: plasmid repositories and standardized plasmid manipulation for molecular and synthetic biology |
| title_full_unstemmed | Tools of the trade: plasmid repositories and standardized plasmid manipulation for molecular and synthetic biology |
| title_short | Tools of the trade: plasmid repositories and standardized plasmid manipulation for molecular and synthetic biology |
| title_sort | tools of the trade plasmid repositories and standardized plasmid manipulation for molecular and synthetic biology |
| url | https://doi.org/10.1111/1751-7915.14012 |
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