Long-Read Sequencing Revealed an Extensive Transcript Complexity in Herpesviruses
Long-read sequencing (LRS) techniques are very recent advancements, but they have already been used for transcriptome research in all of the three subfamilies of herpesviruses. These techniques have multiplied the number of known transcripts in each of the examined viruses. Meanwhile, they have reve...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-07-01
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| Series: | Frontiers in Genetics |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fgene.2018.00259/full |
| _version_ | 1819020342122774528 |
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| author | Dóra Tombácz Zsolt Balázs Zsolt Csabai Michael Snyder Zsolt Boldogkői |
| author_facet | Dóra Tombácz Zsolt Balázs Zsolt Csabai Michael Snyder Zsolt Boldogkői |
| author_sort | Dóra Tombácz |
| collection | DOAJ |
| description | Long-read sequencing (LRS) techniques are very recent advancements, but they have already been used for transcriptome research in all of the three subfamilies of herpesviruses. These techniques have multiplied the number of known transcripts in each of the examined viruses. Meanwhile, they have revealed a so far hidden complexity of the herpesvirus transcriptome with the discovery of a large number of novel RNA molecules, including coding and non-coding RNAs, as well as transcript isoforms, and polycistronic RNAs. Additionally, LRS techniques have uncovered an intricate meshwork of transcriptional overlaps between adjacent and distally located genes. Here, we review the contribution of LRS to herpesvirus transcriptomics and present the complexity revealed by this technology, while also discussing the functional significance of this phenomenon. |
| first_indexed | 2024-12-21T03:49:41Z |
| format | Article |
| id | doaj.art-d75e5b82850e482eb03a44b345df2f94 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-21T03:49:41Z |
| publishDate | 2018-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-d75e5b82850e482eb03a44b345df2f942022-12-21T19:16:59ZengFrontiers Media S.A.Frontiers in Genetics1664-80212018-07-01910.3389/fgene.2018.00259388411Long-Read Sequencing Revealed an Extensive Transcript Complexity in HerpesvirusesDóra Tombácz0Zsolt Balázs1Zsolt Csabai2Michael Snyder3Zsolt Boldogkői4Department of Medical Biology, Faculty of Medicine, University of Szeged, Szeged, HungaryDepartment of Medical Biology, Faculty of Medicine, University of Szeged, Szeged, HungaryDepartment of Medical Biology, Faculty of Medicine, University of Szeged, Szeged, HungaryDepartment of Genetics, School of Medicine, Stanford University, Stanford, CA, United StatesDepartment of Medical Biology, Faculty of Medicine, University of Szeged, Szeged, HungaryLong-read sequencing (LRS) techniques are very recent advancements, but they have already been used for transcriptome research in all of the three subfamilies of herpesviruses. These techniques have multiplied the number of known transcripts in each of the examined viruses. Meanwhile, they have revealed a so far hidden complexity of the herpesvirus transcriptome with the discovery of a large number of novel RNA molecules, including coding and non-coding RNAs, as well as transcript isoforms, and polycistronic RNAs. Additionally, LRS techniques have uncovered an intricate meshwork of transcriptional overlaps between adjacent and distally located genes. Here, we review the contribution of LRS to herpesvirus transcriptomics and present the complexity revealed by this technology, while also discussing the functional significance of this phenomenon.https://www.frontiersin.org/article/10.3389/fgene.2018.00259/fullherpesvirustranscriptomelong-read sequencingPacBio sequencingOxford Nanopore Technologiestranscript isoforms |
| spellingShingle | Dóra Tombácz Zsolt Balázs Zsolt Csabai Michael Snyder Zsolt Boldogkői Long-Read Sequencing Revealed an Extensive Transcript Complexity in Herpesviruses Frontiers in Genetics herpesvirus transcriptome long-read sequencing PacBio sequencing Oxford Nanopore Technologies transcript isoforms |
| title | Long-Read Sequencing Revealed an Extensive Transcript Complexity in Herpesviruses |
| title_full | Long-Read Sequencing Revealed an Extensive Transcript Complexity in Herpesviruses |
| title_fullStr | Long-Read Sequencing Revealed an Extensive Transcript Complexity in Herpesviruses |
| title_full_unstemmed | Long-Read Sequencing Revealed an Extensive Transcript Complexity in Herpesviruses |
| title_short | Long-Read Sequencing Revealed an Extensive Transcript Complexity in Herpesviruses |
| title_sort | long read sequencing revealed an extensive transcript complexity in herpesviruses |
| topic | herpesvirus transcriptome long-read sequencing PacBio sequencing Oxford Nanopore Technologies transcript isoforms |
| url | https://www.frontiersin.org/article/10.3389/fgene.2018.00259/full |
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