Protein A-mouse acidic mammalian chitinase-V5-His expressed in periplasmic space of Escherichia coli possesses chitinase functions comparable to CHO-expressed protein.

Protein A-mouse acidic mammalian chitinase-V5-His expressed in periplasmic space of Escherichia coli possesses chitinase functions comparable to CHO-expressed protein.

Acidic mammalian chitinase (AMCase) has been shown to be associated with asthma in mouse models, allergic inflammation and food processing. Here, we describe an E. coli-expression system that allows for the periplasmic production of active AMCase fused to Protein A at the N-terminus and V5 epitope a...

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Bibliographic Details
Main Authors: Akinori Kashimura, Kazuaki Okawa, Kotarou Ishikawa, Yuta Kida, Kokoro Iwabuchi, Yudai Matsushima, Masayoshi Sakaguchi, Yasusato Sugahara, Fumitaka Oyama
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3823863?pdf=render

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