Characterization of iron-doped crystalline silicon nanoparticles and their modification with citrate anions for in vivo applications
Objectives. This paper presents data on the development and study of the structural properties of iron-doped crystalline silicon (nc-Si/SiOx/Fe) nanoparticles obtained using the plasma-chemical method for application in magnetic resonance imaging diagnostics and treatment of oncological diseases. Th...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
MIREA - Russian Technological University
2021-11-01
|
| Series: | Тонкие химические технологии |
| Subjects: | |
| Online Access: | https://www.finechem-mirea.ru/jour/article/view/1751 |
| _version_ | 1826565685340274688 |
|---|---|
| author | K. I. Rozhkov E. Y. Yagudaeva S. V. Sizova M. A. Lazov E. V. Smirnova V. P. Zubov A. A. Ischenko |
| author_facet | K. I. Rozhkov E. Y. Yagudaeva S. V. Sizova M. A. Lazov E. V. Smirnova V. P. Zubov A. A. Ischenko |
| author_sort | K. I. Rozhkov |
| collection | DOAJ |
| description | Objectives. This paper presents data on the development and study of the structural properties of iron-doped crystalline silicon (nc-Si/SiOx/Fe) nanoparticles obtained using the plasma-chemical method for application in magnetic resonance imaging diagnostics and treatment of oncological diseases. This work aimed to use a variety of analytical methods to study the structural properties of nc-Si/SiOx/Fe and their colloidal stabilization with citrate anions for in vivo applications.Methods. Silicon nanoparticles obtained via the plasma-chemical synthesis method were characterized by laser spark emission spectroscopy, atomic emission spectroscopy, Fouriertransform infrared spectroscopy, and X-ray photoelectron spectroscopy. The hydrodynamic diameter of the nanoparticles was estimated using dynamic light scattering. The toxicity of the nanoparticles was investigated using a colorimetric MTT test for the cell metabolic activity. Elemental iron with different Fe/Si atomic ratios was added to the feedstock during loading.Results. The particles were shown to have a large silicon core covered by a relatively thin layer of intermediate oxides (interface) and an amorphous oxide shell, which is silicon oxide with different oxidation states SiOx (0 ≤ x ≤ 2). The samples had an iron content of 0.8–1.8 at %. Colloidal solutions of the nanoparticles stabilized by citrate anions were obtained and characterized. According to the analysis of the cytotoxicity of the modified nanosilicon particles using monoclonal K562 human erythroleukemia cells, no toxicity was found for cells in culture at particle concentrations of up to 5 µg/mL.Conclusions. Since the obtained modified particles are nontoxic, they can be used in in vivo theranostic applications. |
| first_indexed | 2024-04-10T03:29:07Z |
| format | Article |
| id | doaj.art-d7612695a530493fa4f66785ec99db15 |
| institution | Directory Open Access Journal |
| issn | 2410-6593 2686-7575 |
| language | Russian |
| last_indexed | 2025-03-14T10:39:41Z |
| publishDate | 2021-11-01 |
| publisher | MIREA - Russian Technological University |
| record_format | Article |
| series | Тонкие химические технологии |
| spelling | doaj.art-d7612695a530493fa4f66785ec99db152025-03-02T10:57:37ZrusMIREA - Russian Technological UniversityТонкие химические технологии2410-65932686-75752021-11-0116541442510.32362/2410-6593-2021-16-5-414-4251653Characterization of iron-doped crystalline silicon nanoparticles and their modification with citrate anions for in vivo applicationsK. I. Rozhkov0E. Y. Yagudaeva1S. V. Sizova2M. A. Lazov3E. V. Smirnova4V. P. Zubov5A. A. Ischenko6MIREA – Russian Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies)Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of SciencesShemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of SciencesMIREA – Russian Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies)Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of SciencesMIREA – Russian Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies); Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of SciencesMIREA – Russian Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies)Objectives. This paper presents data on the development and study of the structural properties of iron-doped crystalline silicon (nc-Si/SiOx/Fe) nanoparticles obtained using the plasma-chemical method for application in magnetic resonance imaging diagnostics and treatment of oncological diseases. This work aimed to use a variety of analytical methods to study the structural properties of nc-Si/SiOx/Fe and their colloidal stabilization with citrate anions for in vivo applications.Methods. Silicon nanoparticles obtained via the plasma-chemical synthesis method were characterized by laser spark emission spectroscopy, atomic emission spectroscopy, Fouriertransform infrared spectroscopy, and X-ray photoelectron spectroscopy. The hydrodynamic diameter of the nanoparticles was estimated using dynamic light scattering. The toxicity of the nanoparticles was investigated using a colorimetric MTT test for the cell metabolic activity. Elemental iron with different Fe/Si atomic ratios was added to the feedstock during loading.Results. The particles were shown to have a large silicon core covered by a relatively thin layer of intermediate oxides (interface) and an amorphous oxide shell, which is silicon oxide with different oxidation states SiOx (0 ≤ x ≤ 2). The samples had an iron content of 0.8–1.8 at %. Colloidal solutions of the nanoparticles stabilized by citrate anions were obtained and characterized. According to the analysis of the cytotoxicity of the modified nanosilicon particles using monoclonal K562 human erythroleukemia cells, no toxicity was found for cells in culture at particle concentrations of up to 5 µg/mL.Conclusions. Since the obtained modified particles are nontoxic, they can be used in in vivo theranostic applications.https://www.finechem-mirea.ru/jour/article/view/1751silicon nanoparticlesironmagnetic resonance imagingcitrate anionsx-ray photoelectron spectroscopyfourier-transform infrared spectroscopycytotoxicity |
| spellingShingle | K. I. Rozhkov E. Y. Yagudaeva S. V. Sizova M. A. Lazov E. V. Smirnova V. P. Zubov A. A. Ischenko Characterization of iron-doped crystalline silicon nanoparticles and their modification with citrate anions for in vivo applications Тонкие химические технологии silicon nanoparticles iron magnetic resonance imaging citrate anions x-ray photoelectron spectroscopy fourier-transform infrared spectroscopy cytotoxicity |
| title | Characterization of iron-doped crystalline silicon nanoparticles and their modification with citrate anions for in vivo applications |
| title_full | Characterization of iron-doped crystalline silicon nanoparticles and their modification with citrate anions for in vivo applications |
| title_fullStr | Characterization of iron-doped crystalline silicon nanoparticles and their modification with citrate anions for in vivo applications |
| title_full_unstemmed | Characterization of iron-doped crystalline silicon nanoparticles and their modification with citrate anions for in vivo applications |
| title_short | Characterization of iron-doped crystalline silicon nanoparticles and their modification with citrate anions for in vivo applications |
| title_sort | characterization of iron doped crystalline silicon nanoparticles and their modification with citrate anions for in vivo applications |
| topic | silicon nanoparticles iron magnetic resonance imaging citrate anions x-ray photoelectron spectroscopy fourier-transform infrared spectroscopy cytotoxicity |
| url | https://www.finechem-mirea.ru/jour/article/view/1751 |
| work_keys_str_mv | AT kirozhkov characterizationofirondopedcrystallinesiliconnanoparticlesandtheirmodificationwithcitrateanionsforinvivoapplications AT eyyagudaeva characterizationofirondopedcrystallinesiliconnanoparticlesandtheirmodificationwithcitrateanionsforinvivoapplications AT svsizova characterizationofirondopedcrystallinesiliconnanoparticlesandtheirmodificationwithcitrateanionsforinvivoapplications AT malazov characterizationofirondopedcrystallinesiliconnanoparticlesandtheirmodificationwithcitrateanionsforinvivoapplications AT evsmirnova characterizationofirondopedcrystallinesiliconnanoparticlesandtheirmodificationwithcitrateanionsforinvivoapplications AT vpzubov characterizationofirondopedcrystallinesiliconnanoparticlesandtheirmodificationwithcitrateanionsforinvivoapplications AT aaischenko characterizationofirondopedcrystallinesiliconnanoparticlesandtheirmodificationwithcitrateanionsforinvivoapplications |