MatriGrid<sup>®</sup> Based Biological Morphologies: Tools for 3D Cell Culturing
Recent trends in 3D cell culturing has placed organotypic tissue models at another level. Now, not only is the microenvironment at the cynosure of this research, but rather, microscopic geometrical parameters are also decisive for mimicking a tissue model. Over the years, technologies such as microm...
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MDPI AG
2022-05-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/9/5/220 |
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| author | Patrick Mai Jörg Hampl Martin Baca Dana Brauer Sukhdeep Singh Frank Weise Justyna Borowiec André Schmidt Johanna Merle Küstner Maren Klett Michael Gebinoga Insa S. Schroeder Udo R. Markert Felix Glahn Berit Schumann Diana Eckstein Andreas Schober |
| author_facet | Patrick Mai Jörg Hampl Martin Baca Dana Brauer Sukhdeep Singh Frank Weise Justyna Borowiec André Schmidt Johanna Merle Küstner Maren Klett Michael Gebinoga Insa S. Schroeder Udo R. Markert Felix Glahn Berit Schumann Diana Eckstein Andreas Schober |
| author_sort | Patrick Mai |
| collection | DOAJ |
| description | Recent trends in 3D cell culturing has placed organotypic tissue models at another level. Now, not only is the microenvironment at the cynosure of this research, but rather, microscopic geometrical parameters are also decisive for mimicking a tissue model. Over the years, technologies such as micromachining, 3D printing, and hydrogels are making the foundation of this field. However, mimicking the topography of a particular tissue-relevant substrate can be achieved relatively simply with so-called template or morphology transfer techniques. Over the last 15 years, in one such research venture, we have been investigating a micro thermoforming technique as a facile tool for generating bioinspired topographies. We call them MatriGrid<sup>®</sup>s. In this research account, we summarize our learning outcome from this technique in terms of the influence of 3D micro morphologies on different cell cultures that we have tested in our laboratory. An integral part of this research is the evolution of unavoidable aspects such as possible label-free sensing and fluidic automatization. The development in the research field is also documented in this account. |
| first_indexed | 2024-03-10T03:19:50Z |
| format | Article |
| id | doaj.art-d761935868b74767bcda1e76a6b4bd43 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-10T03:19:50Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-d761935868b74767bcda1e76a6b4bd432023-11-23T10:05:56ZengMDPI AGBioengineering2306-53542022-05-019522010.3390/bioengineering9050220MatriGrid<sup>®</sup> Based Biological Morphologies: Tools for 3D Cell CulturingPatrick Mai0Jörg Hampl1Martin Baca2Dana Brauer3Sukhdeep Singh4Frank Weise5Justyna Borowiec6André Schmidt7Johanna Merle Küstner8Maren Klett9Michael Gebinoga10Insa S. Schroeder11Udo R. Markert12Felix Glahn13Berit Schumann14Diana Eckstein15Andreas Schober16Department of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, 98693 Ilmenau, GermanyDepartment of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, 98693 Ilmenau, GermanyDepartment of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, 98693 Ilmenau, GermanyDepartment of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, 98693 Ilmenau, GermanyDepartment of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, 98693 Ilmenau, GermanyDepartment of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, 98693 Ilmenau, GermanyDepartment of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, 98693 Ilmenau, GermanyPlacenta Lab, Department of Obstetrics, Jena University Hospital, 07747 Jena, GermanyDepartment of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, 98693 Ilmenau, GermanyDepartment of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, 98693 Ilmenau, GermanyDepartment of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, 98693 Ilmenau, GermanyBiophysics Division, GSI Helmholtzzentrum für Schwerionenforschung, 64291 Darmstadt, GermanyPlacenta Lab, Department of Obstetrics, Jena University Hospital, 07747 Jena, GermanyInstitute of Environmental Toxicology, Martin-Luther-University Halle-Wittenberg, 06097 Halle, GermanyInstitute of Environmental Toxicology, Martin-Luther-University Halle-Wittenberg, 06097 Halle, GermanyInstitute of Environmental Toxicology, Martin-Luther-University Halle-Wittenberg, 06097 Halle, GermanyDepartment of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, 98693 Ilmenau, GermanyRecent trends in 3D cell culturing has placed organotypic tissue models at another level. Now, not only is the microenvironment at the cynosure of this research, but rather, microscopic geometrical parameters are also decisive for mimicking a tissue model. Over the years, technologies such as micromachining, 3D printing, and hydrogels are making the foundation of this field. However, mimicking the topography of a particular tissue-relevant substrate can be achieved relatively simply with so-called template or morphology transfer techniques. Over the last 15 years, in one such research venture, we have been investigating a micro thermoforming technique as a facile tool for generating bioinspired topographies. We call them MatriGrid<sup>®</sup>s. In this research account, we summarize our learning outcome from this technique in terms of the influence of 3D micro morphologies on different cell cultures that we have tested in our laboratory. An integral part of this research is the evolution of unavoidable aspects such as possible label-free sensing and fluidic automatization. The development in the research field is also documented in this account.https://www.mdpi.com/2306-5354/9/5/220scaffolds for 3D cell culturehepatocyte culturescaffold manufacturingmanipulation of organoidsstem cell nichesneurons and cerebral bodies |
| spellingShingle | Patrick Mai Jörg Hampl Martin Baca Dana Brauer Sukhdeep Singh Frank Weise Justyna Borowiec André Schmidt Johanna Merle Küstner Maren Klett Michael Gebinoga Insa S. Schroeder Udo R. Markert Felix Glahn Berit Schumann Diana Eckstein Andreas Schober MatriGrid<sup>®</sup> Based Biological Morphologies: Tools for 3D Cell Culturing Bioengineering scaffolds for 3D cell culture hepatocyte culture scaffold manufacturing manipulation of organoids stem cell niches neurons and cerebral bodies |
| title | MatriGrid<sup>®</sup> Based Biological Morphologies: Tools for 3D Cell Culturing |
| title_full | MatriGrid<sup>®</sup> Based Biological Morphologies: Tools for 3D Cell Culturing |
| title_fullStr | MatriGrid<sup>®</sup> Based Biological Morphologies: Tools for 3D Cell Culturing |
| title_full_unstemmed | MatriGrid<sup>®</sup> Based Biological Morphologies: Tools for 3D Cell Culturing |
| title_short | MatriGrid<sup>®</sup> Based Biological Morphologies: Tools for 3D Cell Culturing |
| title_sort | matrigrid sup r sup based biological morphologies tools for 3d cell culturing |
| topic | scaffolds for 3D cell culture hepatocyte culture scaffold manufacturing manipulation of organoids stem cell niches neurons and cerebral bodies |
| url | https://www.mdpi.com/2306-5354/9/5/220 |
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