3-Hydroxy-5,6-epoxy-β-ionone Isolated from Invasive Harmful Brown Seaweed Sargassum Horneri Protects MH-S Mouse Lung Cells from Urban Particulate Matter-Induced Inflammation
Air pollution is a process that mixes pollutants into the atmosphere, which is potentially harmful to humans and causes negative impacts on the surrounding environment (biotic and abiotic). The negative health effects associated with air pollution have been reported from both indoor and outdoor envi...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-11-01
|
| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/11/22/10929 |
| _version_ | 1797511315409862656 |
|---|---|
| author | K. K. Asanka Sanjeewa Hyun-Soo Kim Hyo-Geun Lee Thilina U. Jayawardena D. P. Nagahawatta Hye-Won Yang Dhanushka Udayanga Jae-Il Kim You-Jin Jeon |
| author_facet | K. K. Asanka Sanjeewa Hyun-Soo Kim Hyo-Geun Lee Thilina U. Jayawardena D. P. Nagahawatta Hye-Won Yang Dhanushka Udayanga Jae-Il Kim You-Jin Jeon |
| author_sort | K. K. Asanka Sanjeewa |
| collection | DOAJ |
| description | Air pollution is a process that mixes pollutants into the atmosphere, which is potentially harmful to humans and causes negative impacts on the surrounding environment (biotic and abiotic). The negative health effects associated with air pollution have been reported from both indoor and outdoor environments. Specifically, dust storms originating in Chinese and Mongolian desert areas introduce significant amounts of particulate matter (PM) to the Korean atmosphere. Previously, several studies reported that urban PM (UPM) is a potential agent that causes inflammation in the lungs by altering multiple signal transduction pathways; therefore, screening and identification of anti-inflammatory compounds against UPM-induced inflammation is an urgent requirement. In the present study, we attempted to study the anti-inflammatory properties of 3-Hydroxy-5,6-epoxy-β-ionone (HEBI), a pure compound isolated from invasive brown seaweed, <i>Sargassum horneri</i> (brown edible seaweed), against UPM-stimulated lung macrophages (MH-S). Anti-inflammatory parameters of HEBI were evaluated using Western blots, ELISA, RT-qPCR, and MTT assays. According to the results, HEBI at concentrations between 31.3 and 125 µg/mL reduced UPM-induced NO, PGE2, and pro-inflammatory cytokine production via blocking the downstream signal transduction of NF-κB and MAPKs. Specifically, HEBI down-regulated the mRNA expression levels of Toll-like receptors 2 and 4, which are well-known NF-κB and MAPKs stimulators. Taken together, HEBI is a potential candidate to develop functional foods and active ingredients in cosmeceuticals because of its profound effects against UPM-induced inflammation in MH-S macrophages. |
| first_indexed | 2024-03-10T05:43:37Z |
| format | Article |
| id | doaj.art-d763e776c210454b891e09af3b64a01e |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T05:43:37Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-d763e776c210454b891e09af3b64a01e2023-11-22T22:21:05ZengMDPI AGApplied Sciences2076-34172021-11-0111221092910.3390/app1122109293-Hydroxy-5,6-epoxy-β-ionone Isolated from Invasive Harmful Brown Seaweed Sargassum Horneri Protects MH-S Mouse Lung Cells from Urban Particulate Matter-Induced InflammationK. K. Asanka Sanjeewa0Hyun-Soo Kim1Hyo-Geun Lee2Thilina U. Jayawardena3D. P. Nagahawatta4Hye-Won Yang5Dhanushka Udayanga6Jae-Il Kim7You-Jin Jeon8Department of Biosystems Technology, Faculty of Technology, University of Sri Jayewardenepura, Pitipana, Homagama 10206, Sri LankaDepartment of Marine Life Science, School of Marine Biomedical Sciences, Jeju National University, Jeju 63243, KoreaDepartment of Marine Life Science, School of Marine Biomedical Sciences, Jeju National University, Jeju 63243, KoreaDepartment of Marine Life Science, School of Marine Biomedical Sciences, Jeju National University, Jeju 63243, KoreaDepartment of Marine Life Science, School of Marine Biomedical Sciences, Jeju National University, Jeju 63243, KoreaDepartment of Marine Life Science, School of Marine Biomedical Sciences, Jeju National University, Jeju 63243, KoreaDepartment of Biosystems Technology, Faculty of Technology, University of Sri Jayewardenepura, Pitipana, Homagama 10206, Sri LankaDepartment of Food Science & Nutrition, Pukyong National University, Yongso-ro, 45, Busan 608737, KoreaDepartment of Marine Life Science, School of Marine Biomedical Sciences, Jeju National University, Jeju 63243, KoreaAir pollution is a process that mixes pollutants into the atmosphere, which is potentially harmful to humans and causes negative impacts on the surrounding environment (biotic and abiotic). The negative health effects associated with air pollution have been reported from both indoor and outdoor environments. Specifically, dust storms originating in Chinese and Mongolian desert areas introduce significant amounts of particulate matter (PM) to the Korean atmosphere. Previously, several studies reported that urban PM (UPM) is a potential agent that causes inflammation in the lungs by altering multiple signal transduction pathways; therefore, screening and identification of anti-inflammatory compounds against UPM-induced inflammation is an urgent requirement. In the present study, we attempted to study the anti-inflammatory properties of 3-Hydroxy-5,6-epoxy-β-ionone (HEBI), a pure compound isolated from invasive brown seaweed, <i>Sargassum horneri</i> (brown edible seaweed), against UPM-stimulated lung macrophages (MH-S). Anti-inflammatory parameters of HEBI were evaluated using Western blots, ELISA, RT-qPCR, and MTT assays. According to the results, HEBI at concentrations between 31.3 and 125 µg/mL reduced UPM-induced NO, PGE2, and pro-inflammatory cytokine production via blocking the downstream signal transduction of NF-κB and MAPKs. Specifically, HEBI down-regulated the mRNA expression levels of Toll-like receptors 2 and 4, which are well-known NF-κB and MAPKs stimulators. Taken together, HEBI is a potential candidate to develop functional foods and active ingredients in cosmeceuticals because of its profound effects against UPM-induced inflammation in MH-S macrophages.https://www.mdpi.com/2076-3417/11/22/10929lung macrophagesinflammationoxidative stress<i>Sargassum horneri</i>particulate matter |
| spellingShingle | K. K. Asanka Sanjeewa Hyun-Soo Kim Hyo-Geun Lee Thilina U. Jayawardena D. P. Nagahawatta Hye-Won Yang Dhanushka Udayanga Jae-Il Kim You-Jin Jeon 3-Hydroxy-5,6-epoxy-β-ionone Isolated from Invasive Harmful Brown Seaweed Sargassum Horneri Protects MH-S Mouse Lung Cells from Urban Particulate Matter-Induced Inflammation Applied Sciences lung macrophages inflammation oxidative stress <i>Sargassum horneri</i> particulate matter |
| title | 3-Hydroxy-5,6-epoxy-β-ionone Isolated from Invasive Harmful Brown Seaweed Sargassum Horneri Protects MH-S Mouse Lung Cells from Urban Particulate Matter-Induced Inflammation |
| title_full | 3-Hydroxy-5,6-epoxy-β-ionone Isolated from Invasive Harmful Brown Seaweed Sargassum Horneri Protects MH-S Mouse Lung Cells from Urban Particulate Matter-Induced Inflammation |
| title_fullStr | 3-Hydroxy-5,6-epoxy-β-ionone Isolated from Invasive Harmful Brown Seaweed Sargassum Horneri Protects MH-S Mouse Lung Cells from Urban Particulate Matter-Induced Inflammation |
| title_full_unstemmed | 3-Hydroxy-5,6-epoxy-β-ionone Isolated from Invasive Harmful Brown Seaweed Sargassum Horneri Protects MH-S Mouse Lung Cells from Urban Particulate Matter-Induced Inflammation |
| title_short | 3-Hydroxy-5,6-epoxy-β-ionone Isolated from Invasive Harmful Brown Seaweed Sargassum Horneri Protects MH-S Mouse Lung Cells from Urban Particulate Matter-Induced Inflammation |
| title_sort | 3 hydroxy 5 6 epoxy β ionone isolated from invasive harmful brown seaweed sargassum horneri protects mh s mouse lung cells from urban particulate matter induced inflammation |
| topic | lung macrophages inflammation oxidative stress <i>Sargassum horneri</i> particulate matter |
| url | https://www.mdpi.com/2076-3417/11/22/10929 |
| work_keys_str_mv | AT kkasankasanjeewa 3hydroxy56epoxybiononeisolatedfrominvasiveharmfulbrownseaweedsargassumhorneriprotectsmhsmouselungcellsfromurbanparticulatematterinducedinflammation AT hyunsookim 3hydroxy56epoxybiononeisolatedfrominvasiveharmfulbrownseaweedsargassumhorneriprotectsmhsmouselungcellsfromurbanparticulatematterinducedinflammation AT hyogeunlee 3hydroxy56epoxybiononeisolatedfrominvasiveharmfulbrownseaweedsargassumhorneriprotectsmhsmouselungcellsfromurbanparticulatematterinducedinflammation AT thilinaujayawardena 3hydroxy56epoxybiononeisolatedfrominvasiveharmfulbrownseaweedsargassumhorneriprotectsmhsmouselungcellsfromurbanparticulatematterinducedinflammation AT dpnagahawatta 3hydroxy56epoxybiononeisolatedfrominvasiveharmfulbrownseaweedsargassumhorneriprotectsmhsmouselungcellsfromurbanparticulatematterinducedinflammation AT hyewonyang 3hydroxy56epoxybiononeisolatedfrominvasiveharmfulbrownseaweedsargassumhorneriprotectsmhsmouselungcellsfromurbanparticulatematterinducedinflammation AT dhanushkaudayanga 3hydroxy56epoxybiononeisolatedfrominvasiveharmfulbrownseaweedsargassumhorneriprotectsmhsmouselungcellsfromurbanparticulatematterinducedinflammation AT jaeilkim 3hydroxy56epoxybiononeisolatedfrominvasiveharmfulbrownseaweedsargassumhorneriprotectsmhsmouselungcellsfromurbanparticulatematterinducedinflammation AT youjinjeon 3hydroxy56epoxybiononeisolatedfrominvasiveharmfulbrownseaweedsargassumhorneriprotectsmhsmouselungcellsfromurbanparticulatematterinducedinflammation |