Contents and bioconversion of β-glycoside isoflavones to aglycones in the processing conditions of soybean tempeh

Abstract: The objective of this work was to evaluate the effect of the processing conditions of soybean tempeh on the contents of β-glycoside isoflavones and on their bioconversion into aglycones. Different times of soaking (6, 12, and 18 hours), cooking (15, 30, and 45 minutes), and fermentation (1...

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Main Authors: Cristiane Wing Chong Borges, Mercedes Concórdia Carrão-Panizzi, José Marcos Gontijo Mandarino, Josemeyre Bonifácio da Silva, Silvia Benedetti, Elza Iouko Ida
Format: Article
Language:English
Published: Embrapa Informação Tecnológica 2016-03-01
Series:Pesquisa Agropecuária Brasileira
Subjects:
Online Access:http://www.scielo.br/pdf/pab/v51n3/1678-3921-pab-51-03-00271.pdf
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author Cristiane Wing Chong Borges
Mercedes Concórdia Carrão-Panizzi
José Marcos Gontijo Mandarino
Josemeyre Bonifácio da Silva
Silvia Benedetti
Elza Iouko Ida
author_facet Cristiane Wing Chong Borges
Mercedes Concórdia Carrão-Panizzi
José Marcos Gontijo Mandarino
Josemeyre Bonifácio da Silva
Silvia Benedetti
Elza Iouko Ida
author_sort Cristiane Wing Chong Borges
collection DOAJ
description Abstract: The objective of this work was to evaluate the effect of the processing conditions of soybean tempeh on the contents of β-glycoside isoflavones and on their bioconversion into aglycones. Different times of soaking (6, 12, and 18 hours), cooking (15, 30, and 45 minutes), and fermentation (18, 24, and 30 hours) with Rhizopus oligosporus at 37°C were evaluated for tempeh preparation. Grains from the cultivar 'BRS 267' were used, and the experiment was carried out according to a central composite design (23). The response functions comprised the contents of genistin, malonyldaidzin, malonylgenistin, daidzein, and genistein, quantified by ultraperformance liquid chromatography (UPLC). Soaking, cooking, and fermentation times change the content, profile, and distribution of the different forms of isoflavones in tempeh. The highest bioconversion of glycoside isoflavones into aglycones occurred in 6-hour soaked soybean grains, whose cotyledons were cooked for 15 minutes and subjected to 18-hour fermentation.
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spelling doaj.art-d764dc53db4e420985588a57b8ead4372022-12-21T21:23:57ZengEmbrapa Informação TecnológicaPesquisa Agropecuária Brasileira1678-39212016-03-0151327127910.1590/S0100-204X2016000300009Contents and bioconversion of β-glycoside isoflavones to aglycones in the processing conditions of soybean tempehCristiane Wing Chong BorgesMercedes Concórdia Carrão-PanizziJosé Marcos Gontijo MandarinoJosemeyre Bonifácio da SilvaSilvia BenedettiElza Iouko IdaAbstract: The objective of this work was to evaluate the effect of the processing conditions of soybean tempeh on the contents of β-glycoside isoflavones and on their bioconversion into aglycones. Different times of soaking (6, 12, and 18 hours), cooking (15, 30, and 45 minutes), and fermentation (18, 24, and 30 hours) with Rhizopus oligosporus at 37°C were evaluated for tempeh preparation. Grains from the cultivar 'BRS 267' were used, and the experiment was carried out according to a central composite design (23). The response functions comprised the contents of genistin, malonyldaidzin, malonylgenistin, daidzein, and genistein, quantified by ultraperformance liquid chromatography (UPLC). Soaking, cooking, and fermentation times change the content, profile, and distribution of the different forms of isoflavones in tempeh. The highest bioconversion of glycoside isoflavones into aglycones occurred in 6-hour soaked soybean grains, whose cotyledons were cooked for 15 minutes and subjected to 18-hour fermentation.http://www.scielo.br/pdf/pab/v51n3/1678-3921-pab-51-03-00271.pdfGlycine maxRhizopus oligosporusdaidzeinfermented soybeanfunctional foodgenistein
spellingShingle Cristiane Wing Chong Borges
Mercedes Concórdia Carrão-Panizzi
José Marcos Gontijo Mandarino
Josemeyre Bonifácio da Silva
Silvia Benedetti
Elza Iouko Ida
Contents and bioconversion of β-glycoside isoflavones to aglycones in the processing conditions of soybean tempeh
Pesquisa Agropecuária Brasileira
Glycine max
Rhizopus oligosporus
daidzein
fermented soybean
functional food
genistein
title Contents and bioconversion of β-glycoside isoflavones to aglycones in the processing conditions of soybean tempeh
title_full Contents and bioconversion of β-glycoside isoflavones to aglycones in the processing conditions of soybean tempeh
title_fullStr Contents and bioconversion of β-glycoside isoflavones to aglycones in the processing conditions of soybean tempeh
title_full_unstemmed Contents and bioconversion of β-glycoside isoflavones to aglycones in the processing conditions of soybean tempeh
title_short Contents and bioconversion of β-glycoside isoflavones to aglycones in the processing conditions of soybean tempeh
title_sort contents and bioconversion of β glycoside isoflavones to aglycones in the processing conditions of soybean tempeh
topic Glycine max
Rhizopus oligosporus
daidzein
fermented soybean
functional food
genistein
url http://www.scielo.br/pdf/pab/v51n3/1678-3921-pab-51-03-00271.pdf
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