An RNA-binding protein Blanks plays important roles in defining small RNA and mRNA profiles in Drosophila testes
Drosophila Blanks is a testes-specific RNA-binding protein required for post-meiotic spermiogenesis. However, Blanks's role in regulating RNA populations in the testes remains unknown. We performed small RNA and mRNA high-throughput sequencing in blanks mutant testes and controls. We identified...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-07-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844018317602 |
| _version_ | 1819133412647108608 |
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| author | Susan E. Liao Yiwei Ai Ryuya Fukunaga |
| author_facet | Susan E. Liao Yiwei Ai Ryuya Fukunaga |
| author_sort | Susan E. Liao |
| collection | DOAJ |
| description | Drosophila Blanks is a testes-specific RNA-binding protein required for post-meiotic spermiogenesis. However, Blanks's role in regulating RNA populations in the testes remains unknown. We performed small RNA and mRNA high-throughput sequencing in blanks mutant testes and controls. We identified two miRNAs, one siRNA, and hundreds of mRNAs that are significantly upregulated or downregulated in blanks mutant testes. Pathway analysis revealed that differentially expressed mRNAs are involved in catabolic and metabolic processes, anion and cation transport, mating, and reproductive behavior. Our results reveal that Blanks plays important roles in defining testicular small RNA and mRNA profiles. |
| first_indexed | 2024-12-22T09:46:53Z |
| format | Article |
| id | doaj.art-d766f6a41c7b43d2a21c5cb30d589473 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-22T09:46:53Z |
| publishDate | 2018-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-d766f6a41c7b43d2a21c5cb30d5894732022-12-21T18:30:31ZengElsevierHeliyon2405-84402018-07-0147e00706An RNA-binding protein Blanks plays important roles in defining small RNA and mRNA profiles in Drosophila testesSusan E. Liao0Yiwei Ai1Ryuya Fukunaga2Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 North Wolfe Street, 521A Physiology Building, Baltimore, MD 21205, USADepartment of Biological Chemistry, Johns Hopkins University School of Medicine, 725 North Wolfe Street, 521A Physiology Building, Baltimore, MD 21205, USACorresponding author.; Department of Biological Chemistry, Johns Hopkins University School of Medicine, 725 North Wolfe Street, 521A Physiology Building, Baltimore, MD 21205, USADrosophila Blanks is a testes-specific RNA-binding protein required for post-meiotic spermiogenesis. However, Blanks's role in regulating RNA populations in the testes remains unknown. We performed small RNA and mRNA high-throughput sequencing in blanks mutant testes and controls. We identified two miRNAs, one siRNA, and hundreds of mRNAs that are significantly upregulated or downregulated in blanks mutant testes. Pathway analysis revealed that differentially expressed mRNAs are involved in catabolic and metabolic processes, anion and cation transport, mating, and reproductive behavior. Our results reveal that Blanks plays important roles in defining testicular small RNA and mRNA profiles.http://www.sciencedirect.com/science/article/pii/S2405844018317602GeneticsMolecular biology |
| spellingShingle | Susan E. Liao Yiwei Ai Ryuya Fukunaga An RNA-binding protein Blanks plays important roles in defining small RNA and mRNA profiles in Drosophila testes Heliyon Genetics Molecular biology |
| title | An RNA-binding protein Blanks plays important roles in defining small RNA and mRNA profiles in Drosophila testes |
| title_full | An RNA-binding protein Blanks plays important roles in defining small RNA and mRNA profiles in Drosophila testes |
| title_fullStr | An RNA-binding protein Blanks plays important roles in defining small RNA and mRNA profiles in Drosophila testes |
| title_full_unstemmed | An RNA-binding protein Blanks plays important roles in defining small RNA and mRNA profiles in Drosophila testes |
| title_short | An RNA-binding protein Blanks plays important roles in defining small RNA and mRNA profiles in Drosophila testes |
| title_sort | rna binding protein blanks plays important roles in defining small rna and mrna profiles in drosophila testes |
| topic | Genetics Molecular biology |
| url | http://www.sciencedirect.com/science/article/pii/S2405844018317602 |
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