Efficacy of silver nanoparticles against Trichinella spiralis in mice and the role of multivitamin in alleviating its toxicity
Abstract Trichinellosis is a worldwide zoonotic disease. The majority of currently available anti-trichinellosis medications exhibit inadequate efficacy. The efficacy of a natively prepared new formulation of silver nanoparticles (Ag-NPs) was evaluated in the treatment of Trichinella spiralis (T. sp...
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| Format: | Article |
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Nature Portfolio
2024-03-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-56337-2 |
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| author | Noha Madbouly Taha Fady Sayed Youssef Hend M. Auda Mohamed M. El-Bahy Reem M. Ramadan |
| author_facet | Noha Madbouly Taha Fady Sayed Youssef Hend M. Auda Mohamed M. El-Bahy Reem M. Ramadan |
| author_sort | Noha Madbouly Taha |
| collection | DOAJ |
| description | Abstract Trichinellosis is a worldwide zoonotic disease. The majority of currently available anti-trichinellosis medications exhibit inadequate efficacy. The efficacy of a natively prepared new formulation of silver nanoparticles (Ag-NPs) was evaluated in the treatment of Trichinella spiralis (T. spiralis) infection in mice alone and combined with multivitamin-mineral (MM). After investigating the product’s biological and pharmacological characteristics, its therapeutic dose was estimated to be Ag-NPs at 21.5 mg/kg B.W. This dose was orally inoculated to experimentally infected mice at 3–5 days post-inoculation (dpi) against the mature worms, at 8–10 dpi against the newborn larvae, and at 33–35th dpi against the encapsulated larvae. Each treatment’s efficacy was assessed by scarifying control and treated mice 3 days post-treatment. The drug alone or in supplement form has a high trichinocidal effect exceeding that of the reference drug. Early treatment (3–5 dpi) by Ag-NPs or Ag-NPs + MM and albendazole revealed high efficacy against the intestinal stage, reaching 93.3%, 94.7%, and 90.6% for the three treatments, respectively. The materials causing a significant (P-value < 0.001) decrease in the mean encapsulated larvae reached 86.61%, 89.07%, and 88.84%/gm of muscles using the three treatments, respectively. Moreover, all larvae extracted from Ag-NPs-treated groups failed to induce infection post-inoculation in new mice. Additionally, combining the material with MM proved to overcome the reversible adverse effects of silver material on the estimated redox parameters and liver and kidney biomarkers, denoting its ability to alleviate Ag-NP toxicity. In conclusion, the high trichinocidal effect of Ag-NPs against the adult and encapsulated larvae during a short inoculation period introduced Ag-NPs as an alternative to other nematicidal drugs. |
| first_indexed | 2024-04-24T23:08:33Z |
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| id | doaj.art-d76a2cc85ee946f68e756e74dc6f0dd6 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T23:08:33Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-d76a2cc85ee946f68e756e74dc6f0dd62024-03-17T12:22:45ZengNature PortfolioScientific Reports2045-23222024-03-0114111210.1038/s41598-024-56337-2Efficacy of silver nanoparticles against Trichinella spiralis in mice and the role of multivitamin in alleviating its toxicityNoha Madbouly Taha0Fady Sayed Youssef1Hend M. Auda2Mohamed M. El-Bahy3Reem M. Ramadan4Department of Parasitology, Faculty of Medicine, Cairo UniversityDepartment of Pharmacology, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Parasitology, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Parasitology, Faculty of Veterinary Medicine, Cairo UniversityAbstract Trichinellosis is a worldwide zoonotic disease. The majority of currently available anti-trichinellosis medications exhibit inadequate efficacy. The efficacy of a natively prepared new formulation of silver nanoparticles (Ag-NPs) was evaluated in the treatment of Trichinella spiralis (T. spiralis) infection in mice alone and combined with multivitamin-mineral (MM). After investigating the product’s biological and pharmacological characteristics, its therapeutic dose was estimated to be Ag-NPs at 21.5 mg/kg B.W. This dose was orally inoculated to experimentally infected mice at 3–5 days post-inoculation (dpi) against the mature worms, at 8–10 dpi against the newborn larvae, and at 33–35th dpi against the encapsulated larvae. Each treatment’s efficacy was assessed by scarifying control and treated mice 3 days post-treatment. The drug alone or in supplement form has a high trichinocidal effect exceeding that of the reference drug. Early treatment (3–5 dpi) by Ag-NPs or Ag-NPs + MM and albendazole revealed high efficacy against the intestinal stage, reaching 93.3%, 94.7%, and 90.6% for the three treatments, respectively. The materials causing a significant (P-value < 0.001) decrease in the mean encapsulated larvae reached 86.61%, 89.07%, and 88.84%/gm of muscles using the three treatments, respectively. Moreover, all larvae extracted from Ag-NPs-treated groups failed to induce infection post-inoculation in new mice. Additionally, combining the material with MM proved to overcome the reversible adverse effects of silver material on the estimated redox parameters and liver and kidney biomarkers, denoting its ability to alleviate Ag-NP toxicity. In conclusion, the high trichinocidal effect of Ag-NPs against the adult and encapsulated larvae during a short inoculation period introduced Ag-NPs as an alternative to other nematicidal drugs.https://doi.org/10.1038/s41598-024-56337-2Trichinella spiralisSilver nanoparticlesToxicityMultivitamin |
| spellingShingle | Noha Madbouly Taha Fady Sayed Youssef Hend M. Auda Mohamed M. El-Bahy Reem M. Ramadan Efficacy of silver nanoparticles against Trichinella spiralis in mice and the role of multivitamin in alleviating its toxicity Scientific Reports Trichinella spiralis Silver nanoparticles Toxicity Multivitamin |
| title | Efficacy of silver nanoparticles against Trichinella spiralis in mice and the role of multivitamin in alleviating its toxicity |
| title_full | Efficacy of silver nanoparticles against Trichinella spiralis in mice and the role of multivitamin in alleviating its toxicity |
| title_fullStr | Efficacy of silver nanoparticles against Trichinella spiralis in mice and the role of multivitamin in alleviating its toxicity |
| title_full_unstemmed | Efficacy of silver nanoparticles against Trichinella spiralis in mice and the role of multivitamin in alleviating its toxicity |
| title_short | Efficacy of silver nanoparticles against Trichinella spiralis in mice and the role of multivitamin in alleviating its toxicity |
| title_sort | efficacy of silver nanoparticles against trichinella spiralis in mice and the role of multivitamin in alleviating its toxicity |
| topic | Trichinella spiralis Silver nanoparticles Toxicity Multivitamin |
| url | https://doi.org/10.1038/s41598-024-56337-2 |
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