Optimization of Molecularly Imprinted Solid Phase Extraction-High Performance Liquid Chromatography and Its Application in Determination of Benzo (a) pyrene in Vegetable Oil
An optimized method for the determination of benzopyrene in vegetable oil by molecular imprinting solid phase extraction with ultra-high performance liquid chromatography was established. During the experiment, through the investigation of different solid-phase extraction purification efficiency, op...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Academy of National Food and Strategic Reserves Administration
2023-01-01
|
| Series: | Liang you shipin ke-ji |
| Subjects: | |
| Online Access: | http://lyspkj.ijournal.cn/lyspkj/article/abstract/20230120?st=article_issue |
| _version_ | 1797895437883015168 |
|---|---|
| author | LI Wen-ting LIU Ling XIA Jia-en DONG Yu-ying RAN Ya-li ZHAO Li |
| author_facet | LI Wen-ting LIU Ling XIA Jia-en DONG Yu-ying RAN Ya-li ZHAO Li |
| author_sort | LI Wen-ting |
| collection | DOAJ |
| description | An optimized method for the determination of benzopyrene in vegetable oil by molecular imprinting solid phase extraction with ultra-high performance liquid chromatography was established. During the experiment, through the investigation of different solid-phase extraction purification efficiency, optimal detection wavelength, mobile phase system and chromatographic column use, the results showed that the molecularly imprinted solid phase extraction purification column was more suitable for pretreatment extraction and purification of vegetable oil. The optimal detection the excitation wavelength and the emission wavelength were 365 nm and 410 nm, respectively. Under these conditions, the benzopyrene chromatographic peak had high response value and signal-to-noise ratio. Under the mobile phase system of 90% acetonitrile-10% water, the target peak had a good separation effect. Using 150 mm short columns can effectively improve work efficiency. Benzopyrene had a good linear relationship in the concentration range of 0.3~20 µg/L, and the correlation coefficient was greater than 0.999 9. The detection limit and quantitative limit of benzopyrene determination method were 0.1 µg/kg and 0.3 µg/kg, respectively. The average recoveries of three added concentrations were 87.6%~95.9%, and the relative standard deviations were 1.08%~2.16%. In the actual sample detection, the detection rate of benzopyrene, the exceeding rate and the qualified rate were 39.0%, 2.4%, and was 97.6%, respectively. This optimization method is sensitive, convenient, rapid and accurate, and can be used for batch detection of benzopyrene in vegetable oil and analysis of pollution monitoring. |
| first_indexed | 2024-04-10T07:26:50Z |
| format | Article |
| id | doaj.art-d76f1d88a1624c5ead244ae2d85900d5 |
| institution | Directory Open Access Journal |
| issn | 1007-7561 |
| language | English |
| last_indexed | 2024-04-10T07:26:50Z |
| publishDate | 2023-01-01 |
| publisher | Academy of National Food and Strategic Reserves Administration |
| record_format | Article |
| series | Liang you shipin ke-ji |
| spelling | doaj.art-d76f1d88a1624c5ead244ae2d85900d52023-02-24T06:24:36ZengAcademy of National Food and Strategic Reserves AdministrationLiang you shipin ke-ji1007-75612023-01-0131115015710.16210/j.cnki.1007-7561.2023.01.019Optimization of Molecularly Imprinted Solid Phase Extraction-High Performance Liquid Chromatography and Its Application in Determination of Benzo (a) pyrene in Vegetable OilLI Wen-ting0LIU Ling1XIA Jia-en2DONG Yu-ying3RAN Ya-li4ZHAO Li5 Kunming Center for Disease Control and Prevention, Kunming, Yunnan 650228, ChinaKunming Center for Disease Control and Prevention, Kunming, Yunnan 650228, China;School of Ethnic Medicine, Yunnan Minzu Uinversity, Kunming, Yunnan 650500, ChinaKunming Dongchuan District Center for Disease Control and Prevention, Kunming, Yunnan 650228, ChinaKunming Center for Disease Control and Prevention, Kunming, Yunnan 650228, ChinaKunming Center for Disease Control and Prevention, Kunming, Yunnan 650228, ChinaKunming Center for Disease Control and Prevention, Kunming, Yunnan 650228, ChinaAn optimized method for the determination of benzopyrene in vegetable oil by molecular imprinting solid phase extraction with ultra-high performance liquid chromatography was established. During the experiment, through the investigation of different solid-phase extraction purification efficiency, optimal detection wavelength, mobile phase system and chromatographic column use, the results showed that the molecularly imprinted solid phase extraction purification column was more suitable for pretreatment extraction and purification of vegetable oil. The optimal detection the excitation wavelength and the emission wavelength were 365 nm and 410 nm, respectively. Under these conditions, the benzopyrene chromatographic peak had high response value and signal-to-noise ratio. Under the mobile phase system of 90% acetonitrile-10% water, the target peak had a good separation effect. Using 150 mm short columns can effectively improve work efficiency. Benzopyrene had a good linear relationship in the concentration range of 0.3~20 µg/L, and the correlation coefficient was greater than 0.999 9. The detection limit and quantitative limit of benzopyrene determination method were 0.1 µg/kg and 0.3 µg/kg, respectively. The average recoveries of three added concentrations were 87.6%~95.9%, and the relative standard deviations were 1.08%~2.16%. In the actual sample detection, the detection rate of benzopyrene, the exceeding rate and the qualified rate were 39.0%, 2.4%, and was 97.6%, respectively. This optimization method is sensitive, convenient, rapid and accurate, and can be used for batch detection of benzopyrene in vegetable oil and analysis of pollution monitoring.http://lyspkj.ijournal.cn/lyspkj/article/abstract/20230120?st=article_issuebenzopyrenemolecular imprinting columnvegetable oilssolid phase extractionhigh performance liquid chromatography |
| spellingShingle | LI Wen-ting LIU Ling XIA Jia-en DONG Yu-ying RAN Ya-li ZHAO Li Optimization of Molecularly Imprinted Solid Phase Extraction-High Performance Liquid Chromatography and Its Application in Determination of Benzo (a) pyrene in Vegetable Oil Liang you shipin ke-ji benzopyrene molecular imprinting column vegetable oils solid phase extraction high performance liquid chromatography |
| title | Optimization of Molecularly Imprinted Solid Phase Extraction-High Performance Liquid Chromatography and Its Application in Determination of Benzo (a) pyrene in Vegetable Oil |
| title_full | Optimization of Molecularly Imprinted Solid Phase Extraction-High Performance Liquid Chromatography and Its Application in Determination of Benzo (a) pyrene in Vegetable Oil |
| title_fullStr | Optimization of Molecularly Imprinted Solid Phase Extraction-High Performance Liquid Chromatography and Its Application in Determination of Benzo (a) pyrene in Vegetable Oil |
| title_full_unstemmed | Optimization of Molecularly Imprinted Solid Phase Extraction-High Performance Liquid Chromatography and Its Application in Determination of Benzo (a) pyrene in Vegetable Oil |
| title_short | Optimization of Molecularly Imprinted Solid Phase Extraction-High Performance Liquid Chromatography and Its Application in Determination of Benzo (a) pyrene in Vegetable Oil |
| title_sort | optimization of molecularly imprinted solid phase extraction high performance liquid chromatography and its application in determination of benzo a pyrene in vegetable oil |
| topic | benzopyrene molecular imprinting column vegetable oils solid phase extraction high performance liquid chromatography |
| url | http://lyspkj.ijournal.cn/lyspkj/article/abstract/20230120?st=article_issue |
| work_keys_str_mv | AT liwenting optimizationofmolecularlyimprintedsolidphaseextractionhighperformanceliquidchromatographyanditsapplicationindeterminationofbenzoapyreneinvegetableoil AT liuling optimizationofmolecularlyimprintedsolidphaseextractionhighperformanceliquidchromatographyanditsapplicationindeterminationofbenzoapyreneinvegetableoil AT xiajiaen optimizationofmolecularlyimprintedsolidphaseextractionhighperformanceliquidchromatographyanditsapplicationindeterminationofbenzoapyreneinvegetableoil AT dongyuying optimizationofmolecularlyimprintedsolidphaseextractionhighperformanceliquidchromatographyanditsapplicationindeterminationofbenzoapyreneinvegetableoil AT ranyali optimizationofmolecularlyimprintedsolidphaseextractionhighperformanceliquidchromatographyanditsapplicationindeterminationofbenzoapyreneinvegetableoil AT zhaoli optimizationofmolecularlyimprintedsolidphaseextractionhighperformanceliquidchromatographyanditsapplicationindeterminationofbenzoapyreneinvegetableoil |