Distinct Characteristics of <i>Escherichia coli</i> Isolated from Patients with Urinary Tract Infections in a Medical Center at a Ten-Year Interval
<i>Escherichia coli</i> causing urinary tract infections (UTIs) are one of the most common outpatient bacterial infections. This study aimed to compare the characteristics of <i>E. coli</i> isolated from UTI patients in a single medical center in 2009–2010 (n = 504) and 2020...
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| Format: | Article |
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MDPI AG
2021-09-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/10/9/1156 |
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| author | Wei-Hung Lin Yen-Zhen Zhang Po-Yao Liu Po-Shun Chen Shining Wang Pei-Yun Kuo Tran Thi Dieu Thuy Tran Thi Thuy Duong Li-Li Wen Yi-Hsien Hsieh Ming-Cheng Wang Cheng-Yen Kao |
| author_facet | Wei-Hung Lin Yen-Zhen Zhang Po-Yao Liu Po-Shun Chen Shining Wang Pei-Yun Kuo Tran Thi Dieu Thuy Tran Thi Thuy Duong Li-Li Wen Yi-Hsien Hsieh Ming-Cheng Wang Cheng-Yen Kao |
| author_sort | Wei-Hung Lin |
| collection | DOAJ |
| description | <i>Escherichia coli</i> causing urinary tract infections (UTIs) are one of the most common outpatient bacterial infections. This study aimed to compare the characteristics of <i>E. coli</i> isolated from UTI patients in a single medical center in 2009–2010 (n = 504) and 2020 (n = 340). The antimicrobial susceptibility of <i>E. coli</i> was determined by the disk diffusion method. PCRs were conducted to detect phylogenetic groups, ST131, K1 capsule antigen, and 15 virulence factors. Phylogenetic group B2 dominated in our 2009–2010 and 2020 isolates. Moreover, no phylogenetic group E strains were isolated in 2020. <i>E. coli</i> isolates in 2020 were more susceptible to amoxicillin, ampicillin/sulbactam, cefuroxime, cefmetazole, ceftazidime, cefoxitin, tetracycline, and sulfamethoxazole/trimethoprim, compared to the isolates in 2009–2010. Extensively drug-resistant (XDR)-<i>E. coli</i> in 2009–2010 were detected in groups B1 (5 isolates), B2 (12 isolates), F (8 isolates), and unknown (1 isolate). In 2020, XDR-<i>E. coli</i> were only detected in groups A (2 isolates), B2 (5 isolates), D (1 isolate), and F (4 isolates). The prevalence of virulence factor genes <i>aer</i> and <i>fimH</i> were higher in <i>E. coli</i> in 2009–2010 compared to those in 2020. In contrast, <i>afa</i> and <i>sat</i> showed higher frequencies in <i>E. coli</i> isolates in 2020 compared to <i>E. coli</i> in 2009–2010. |
| first_indexed | 2024-03-10T07:19:41Z |
| format | Article |
| id | doaj.art-d77083498865495b8131768695d449be |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T07:19:41Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-d77083498865495b8131768695d449be2023-11-22T14:42:36ZengMDPI AGPathogens2076-08172021-09-01109115610.3390/pathogens10091156Distinct Characteristics of <i>Escherichia coli</i> Isolated from Patients with Urinary Tract Infections in a Medical Center at a Ten-Year IntervalWei-Hung Lin0Yen-Zhen Zhang1Po-Yao Liu2Po-Shun Chen3Shining Wang4Pei-Yun Kuo5Tran Thi Dieu Thuy6Tran Thi Thuy Duong7Li-Li Wen8Yi-Hsien Hsieh9Ming-Cheng Wang10Cheng-Yen Kao11Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan 701, TaiwanInstitute of Microbiology and Immunology, School of Life Sciences, National Yang Ming Chiao Tung University, Taipei 112, TaiwanInstitute of Microbiology and Immunology, School of Life Sciences, National Yang Ming Chiao Tung University, Taipei 112, TaiwanDepartment of Biomedical Engineering, University of Southern California, Los Angeles, CA 90089-0482, USAInstitute of Microbiology and Immunology, School of Life Sciences, National Yang Ming Chiao Tung University, Taipei 112, TaiwanInstitute of Microbiology and Immunology, School of Life Sciences, National Yang Ming Chiao Tung University, Taipei 112, TaiwanInstitute of Microbiology and Immunology, School of Life Sciences, National Yang Ming Chiao Tung University, Taipei 112, TaiwanInstitute of Microbiology and Immunology, School of Life Sciences, National Yang Ming Chiao Tung University, Taipei 112, TaiwanDepartment of Clinical Laboratory, En Chu Kong Hospital, New Taipei City 237, TaiwanInstitute of Medicine, Chung Shan Medical University, Taichung 402, TaiwanDivision of Nephrology, Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 701, TaiwanInstitute of Microbiology and Immunology, School of Life Sciences, National Yang Ming Chiao Tung University, Taipei 112, Taiwan<i>Escherichia coli</i> causing urinary tract infections (UTIs) are one of the most common outpatient bacterial infections. This study aimed to compare the characteristics of <i>E. coli</i> isolated from UTI patients in a single medical center in 2009–2010 (n = 504) and 2020 (n = 340). The antimicrobial susceptibility of <i>E. coli</i> was determined by the disk diffusion method. PCRs were conducted to detect phylogenetic groups, ST131, K1 capsule antigen, and 15 virulence factors. Phylogenetic group B2 dominated in our 2009–2010 and 2020 isolates. Moreover, no phylogenetic group E strains were isolated in 2020. <i>E. coli</i> isolates in 2020 were more susceptible to amoxicillin, ampicillin/sulbactam, cefuroxime, cefmetazole, ceftazidime, cefoxitin, tetracycline, and sulfamethoxazole/trimethoprim, compared to the isolates in 2009–2010. Extensively drug-resistant (XDR)-<i>E. coli</i> in 2009–2010 were detected in groups B1 (5 isolates), B2 (12 isolates), F (8 isolates), and unknown (1 isolate). In 2020, XDR-<i>E. coli</i> were only detected in groups A (2 isolates), B2 (5 isolates), D (1 isolate), and F (4 isolates). The prevalence of virulence factor genes <i>aer</i> and <i>fimH</i> were higher in <i>E. coli</i> in 2009–2010 compared to those in 2020. In contrast, <i>afa</i> and <i>sat</i> showed higher frequencies in <i>E. coli</i> isolates in 2020 compared to <i>E. coli</i> in 2009–2010.https://www.mdpi.com/2076-0817/10/9/1156antimicrobial susceptibilitylongitudinal surveillancephylogenetic groupsurinary tract infectionsvirulence factors |
| spellingShingle | Wei-Hung Lin Yen-Zhen Zhang Po-Yao Liu Po-Shun Chen Shining Wang Pei-Yun Kuo Tran Thi Dieu Thuy Tran Thi Thuy Duong Li-Li Wen Yi-Hsien Hsieh Ming-Cheng Wang Cheng-Yen Kao Distinct Characteristics of <i>Escherichia coli</i> Isolated from Patients with Urinary Tract Infections in a Medical Center at a Ten-Year Interval Pathogens antimicrobial susceptibility longitudinal surveillance phylogenetic groups urinary tract infections virulence factors |
| title | Distinct Characteristics of <i>Escherichia coli</i> Isolated from Patients with Urinary Tract Infections in a Medical Center at a Ten-Year Interval |
| title_full | Distinct Characteristics of <i>Escherichia coli</i> Isolated from Patients with Urinary Tract Infections in a Medical Center at a Ten-Year Interval |
| title_fullStr | Distinct Characteristics of <i>Escherichia coli</i> Isolated from Patients with Urinary Tract Infections in a Medical Center at a Ten-Year Interval |
| title_full_unstemmed | Distinct Characteristics of <i>Escherichia coli</i> Isolated from Patients with Urinary Tract Infections in a Medical Center at a Ten-Year Interval |
| title_short | Distinct Characteristics of <i>Escherichia coli</i> Isolated from Patients with Urinary Tract Infections in a Medical Center at a Ten-Year Interval |
| title_sort | distinct characteristics of i escherichia coli i isolated from patients with urinary tract infections in a medical center at a ten year interval |
| topic | antimicrobial susceptibility longitudinal surveillance phylogenetic groups urinary tract infections virulence factors |
| url | https://www.mdpi.com/2076-0817/10/9/1156 |
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