Variation in the CENP-A sequence association landscape across diverse inbred mouse strains
Summary: Centromeres are crucial for chromosome segregation, but their underlying sequences evolve rapidly, imposing strong selection for compensatory changes in centromere-associated kinetochore proteins to assure the stability of genome transmission. While this co-evolution is well documented betw...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124723011907 |
| _version_ | 1797674933662253056 |
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| author | Uma P. Arora Beth A. Sullivan Beth L. Dumont |
| author_facet | Uma P. Arora Beth A. Sullivan Beth L. Dumont |
| author_sort | Uma P. Arora |
| collection | DOAJ |
| description | Summary: Centromeres are crucial for chromosome segregation, but their underlying sequences evolve rapidly, imposing strong selection for compensatory changes in centromere-associated kinetochore proteins to assure the stability of genome transmission. While this co-evolution is well documented between species, it remains unknown whether population-level centromere diversity leads to functional differences in kinetochore protein association. Mice (Mus musculus) exhibit remarkable variation in centromere size and sequence, but the amino acid sequence of the kinetochore protein CENP-A is conserved. Here, we apply k-mer-based analyses to CENP-A chromatin profiling data from diverse inbred mouse strains to investigate the interplay between centromere variation and kinetochore protein sequence association. We show that centromere sequence diversity is associated with strain-level differences in both CENP-A positioning and sequence preference along the mouse core centromere satellite. Our findings reveal intraspecies sequence-dependent differences in CENP-A/centromere association and open additional perspectives for understanding centromere-mediated variation in genome stability. |
| first_indexed | 2024-03-11T22:07:20Z |
| format | Article |
| id | doaj.art-d7713c1ce6394892850c00157582d497 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-03-11T22:07:20Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-d7713c1ce6394892850c00157582d4972023-09-25T04:12:11ZengElsevierCell Reports2211-12472023-10-014210113178Variation in the CENP-A sequence association landscape across diverse inbred mouse strainsUma P. Arora0Beth A. Sullivan1Beth L. Dumont2The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609, USA; Graduate School of Biomedical Sciences, Tufts University, 136 Harrison Avenue, Boston, MA 02111, USA; Corresponding authorDepartment of Molecular Genetics and Microbiology, Duke University Medical Center, 213 Research Drive, Box 3054, Durham, NC 27710, USAThe Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609, USA; Graduate School of Biomedical Sciences, Tufts University, 136 Harrison Avenue, Boston, MA 02111, USA; Graduate School of Biomedical Science and Engineering, University of Maine, 5775 Stodder Hall, Room 46, Orono, ME 04469, USA; Corresponding authorSummary: Centromeres are crucial for chromosome segregation, but their underlying sequences evolve rapidly, imposing strong selection for compensatory changes in centromere-associated kinetochore proteins to assure the stability of genome transmission. While this co-evolution is well documented between species, it remains unknown whether population-level centromere diversity leads to functional differences in kinetochore protein association. Mice (Mus musculus) exhibit remarkable variation in centromere size and sequence, but the amino acid sequence of the kinetochore protein CENP-A is conserved. Here, we apply k-mer-based analyses to CENP-A chromatin profiling data from diverse inbred mouse strains to investigate the interplay between centromere variation and kinetochore protein sequence association. We show that centromere sequence diversity is associated with strain-level differences in both CENP-A positioning and sequence preference along the mouse core centromere satellite. Our findings reveal intraspecies sequence-dependent differences in CENP-A/centromere association and open additional perspectives for understanding centromere-mediated variation in genome stability.http://www.sciencedirect.com/science/article/pii/S2211124723011907CP: Genomics |
| spellingShingle | Uma P. Arora Beth A. Sullivan Beth L. Dumont Variation in the CENP-A sequence association landscape across diverse inbred mouse strains Cell Reports CP: Genomics |
| title | Variation in the CENP-A sequence association landscape across diverse inbred mouse strains |
| title_full | Variation in the CENP-A sequence association landscape across diverse inbred mouse strains |
| title_fullStr | Variation in the CENP-A sequence association landscape across diverse inbred mouse strains |
| title_full_unstemmed | Variation in the CENP-A sequence association landscape across diverse inbred mouse strains |
| title_short | Variation in the CENP-A sequence association landscape across diverse inbred mouse strains |
| title_sort | variation in the cenp a sequence association landscape across diverse inbred mouse strains |
| topic | CP: Genomics |
| url | http://www.sciencedirect.com/science/article/pii/S2211124723011907 |
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