Peduncle Necking in Rosa hybrida Induces Stress-Related Transcription Factors, Upregulates Galactose Metabolism, and Downregulates Phenylpropanoid Biosynthesis Genes
Roses are highly valued as cut flowers worldwide but have limited vase life. Peduncle bending “bent neck” or “necking” is a major cause of reduced vase life, especially in some cultivars. Necking is thought to be caused by either an air embolism or accumulation of microorganisms at or within the ste...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.874590/full |
| _version_ | 1811300347183890432 |
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| author | Bianca Lear Matthew Casey Anthony D. Stead Hilary Joan Rogers |
| author_facet | Bianca Lear Matthew Casey Anthony D. Stead Hilary Joan Rogers |
| author_sort | Bianca Lear |
| collection | DOAJ |
| description | Roses are highly valued as cut flowers worldwide but have limited vase life. Peduncle bending “bent neck” or “necking” is a major cause of reduced vase life, especially in some cultivars. Necking is thought to be caused by either an air embolism or accumulation of microorganisms at or within the stem end, blocking the xylem vessels and preventing water uptake. However, the underlying mechanisms of necking are poorly understood. Here, RNAseq analysis was applied to compare gene expression across three stages of peduncle necking (straight, <90°, and >90°), in the necking-susceptible Rosa hybrida cultivar H30. Most gene expression change was later in bending and there was, overall, more downregulation than upregulation of gene expression during necking. Photosynthetic, starch, and lignin biosynthesis genes were all downregulated, while genes associated with galactose metabolism, producing raffinose and trehalose that are both related to osmoprotection, were upregulated. Genes associated with starch breakdown, autophagy, and senescence were also upregulated, as were most of the NAC and WRKY transcription factors, involved in stress and senescence regulation. Microscopy showed a cellular collapse in the peduncle. These data support a possible mechanism, whereby a reduction in water transport leads to a cellular collapse in the peduncle, accompanied by upregulation of senescence and drought responses. |
| first_indexed | 2024-04-13T06:49:43Z |
| format | Article |
| id | doaj.art-d7737231ff544f1dafe052665b996334 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T06:49:43Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-d7737231ff544f1dafe052665b9963342022-12-22T02:57:27ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-04-011310.3389/fpls.2022.874590874590Peduncle Necking in Rosa hybrida Induces Stress-Related Transcription Factors, Upregulates Galactose Metabolism, and Downregulates Phenylpropanoid Biosynthesis GenesBianca Lear0Matthew Casey1Anthony D. Stead2Hilary Joan Rogers3School of Biological Sciences, Royal Holloway University of London, Egham, United KingdomSchool of Biological Sciences, Royal Holloway University of London, Egham, United KingdomSchool of Biological Sciences, Royal Holloway University of London, Egham, United KingdomSchool of Biosciences, Cardiff University, Cardiff, United KingdomRoses are highly valued as cut flowers worldwide but have limited vase life. Peduncle bending “bent neck” or “necking” is a major cause of reduced vase life, especially in some cultivars. Necking is thought to be caused by either an air embolism or accumulation of microorganisms at or within the stem end, blocking the xylem vessels and preventing water uptake. However, the underlying mechanisms of necking are poorly understood. Here, RNAseq analysis was applied to compare gene expression across three stages of peduncle necking (straight, <90°, and >90°), in the necking-susceptible Rosa hybrida cultivar H30. Most gene expression change was later in bending and there was, overall, more downregulation than upregulation of gene expression during necking. Photosynthetic, starch, and lignin biosynthesis genes were all downregulated, while genes associated with galactose metabolism, producing raffinose and trehalose that are both related to osmoprotection, were upregulated. Genes associated with starch breakdown, autophagy, and senescence were also upregulated, as were most of the NAC and WRKY transcription factors, involved in stress and senescence regulation. Microscopy showed a cellular collapse in the peduncle. These data support a possible mechanism, whereby a reduction in water transport leads to a cellular collapse in the peduncle, accompanied by upregulation of senescence and drought responses.https://www.frontiersin.org/articles/10.3389/fpls.2022.874590/fullfloral senescencepeduncleRosa hybridastress biologytranscriptome |
| spellingShingle | Bianca Lear Matthew Casey Anthony D. Stead Hilary Joan Rogers Peduncle Necking in Rosa hybrida Induces Stress-Related Transcription Factors, Upregulates Galactose Metabolism, and Downregulates Phenylpropanoid Biosynthesis Genes Frontiers in Plant Science floral senescence peduncle Rosa hybrida stress biology transcriptome |
| title | Peduncle Necking in Rosa hybrida Induces Stress-Related Transcription Factors, Upregulates Galactose Metabolism, and Downregulates Phenylpropanoid Biosynthesis Genes |
| title_full | Peduncle Necking in Rosa hybrida Induces Stress-Related Transcription Factors, Upregulates Galactose Metabolism, and Downregulates Phenylpropanoid Biosynthesis Genes |
| title_fullStr | Peduncle Necking in Rosa hybrida Induces Stress-Related Transcription Factors, Upregulates Galactose Metabolism, and Downregulates Phenylpropanoid Biosynthesis Genes |
| title_full_unstemmed | Peduncle Necking in Rosa hybrida Induces Stress-Related Transcription Factors, Upregulates Galactose Metabolism, and Downregulates Phenylpropanoid Biosynthesis Genes |
| title_short | Peduncle Necking in Rosa hybrida Induces Stress-Related Transcription Factors, Upregulates Galactose Metabolism, and Downregulates Phenylpropanoid Biosynthesis Genes |
| title_sort | peduncle necking in rosa hybrida induces stress related transcription factors upregulates galactose metabolism and downregulates phenylpropanoid biosynthesis genes |
| topic | floral senescence peduncle Rosa hybrida stress biology transcriptome |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.874590/full |
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