Comparative Proteomic Analysis of an Ethyl Tert-Butyl Ether-Degrading Bacterial Consortium
A bacterial consortium capable of degrading ethyl <i>tert</i>-butyl ether (ETBE) as a sole carbon source was enriched and isolated from gasoline-contaminated water. <i>Arthrobacter</i> sp., <i>Herbaspirillum</i> sp., <i>Pseudacidovorax</i> sp., <i&g...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/12/2331 |
| _version_ | 1797456253771841536 |
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| author | Vijayalakshmi Gunasekaran Núria Canela Magda Constantí |
| author_facet | Vijayalakshmi Gunasekaran Núria Canela Magda Constantí |
| author_sort | Vijayalakshmi Gunasekaran |
| collection | DOAJ |
| description | A bacterial consortium capable of degrading ethyl <i>tert</i>-butyl ether (ETBE) as a sole carbon source was enriched and isolated from gasoline-contaminated water. <i>Arthrobacter</i> sp., <i>Herbaspirillum</i> sp., <i>Pseudacidovorax</i> sp., <i>Pseudomonas</i> sp., and <i>Xanthomonas</i> sp. were identified as the initial populations with the 16S rDNA analysis. The consortium aerobically degraded 49% of 50 mg/L of ETBE, in 6 days. The ETBE degrading efficiency of the consortium increased to 98% even with the higher concentrations of ETBE (1000 mg/L) in the subsequent subcultures, which accumulated tert-butyl alcohol (TBA). <i>Xanthomonas</i> sp. and <i>Pseudomonas</i> sp. were identified as the predominant ETBE degrading populations in the final subculture. The metaproteome of the ETBE-grown bacterial consortium was compared with the glucose-grown bacterial consortium, using 2D-DIGE. Proteins related to the ETBE metabolism, stress response, carbon metabolism and chaperones were found to be abundant in the presence of ETBE while proteins related to cell division were less abundant. The metaproteomic study revealed that the ETBE does have an effect on the metabolism of the bacterial consortium. It also enabled us to understand the responses of the complex bacterial consortium to ETBE, thus revealing interesting facts about the ETBE degrading bacterial community. |
| first_indexed | 2024-03-09T16:05:35Z |
| format | Article |
| id | doaj.art-d7758d0f9a404f188cf08c2336dac799 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T16:05:35Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-d7758d0f9a404f188cf08c2336dac7992023-11-24T16:47:18ZengMDPI AGMicroorganisms2076-26072022-11-011012233110.3390/microorganisms10122331Comparative Proteomic Analysis of an Ethyl Tert-Butyl Ether-Degrading Bacterial ConsortiumVijayalakshmi Gunasekaran0Núria Canela1Magda Constantí2Departament d’Enginyeria Química, Universitat Rovira i Virgili, Av. Països Catalans, 26, 43007 Tarragona, SpainCentre for Omic Sciences (COS), Joint Unit Universitat Rovira i Virgili-EURECAT, Av. Universitat 1, 43204 Reus, SpainDepartament d’Enginyeria Química, Universitat Rovira i Virgili, Av. Països Catalans, 26, 43007 Tarragona, SpainA bacterial consortium capable of degrading ethyl <i>tert</i>-butyl ether (ETBE) as a sole carbon source was enriched and isolated from gasoline-contaminated water. <i>Arthrobacter</i> sp., <i>Herbaspirillum</i> sp., <i>Pseudacidovorax</i> sp., <i>Pseudomonas</i> sp., and <i>Xanthomonas</i> sp. were identified as the initial populations with the 16S rDNA analysis. The consortium aerobically degraded 49% of 50 mg/L of ETBE, in 6 days. The ETBE degrading efficiency of the consortium increased to 98% even with the higher concentrations of ETBE (1000 mg/L) in the subsequent subcultures, which accumulated tert-butyl alcohol (TBA). <i>Xanthomonas</i> sp. and <i>Pseudomonas</i> sp. were identified as the predominant ETBE degrading populations in the final subculture. The metaproteome of the ETBE-grown bacterial consortium was compared with the glucose-grown bacterial consortium, using 2D-DIGE. Proteins related to the ETBE metabolism, stress response, carbon metabolism and chaperones were found to be abundant in the presence of ETBE while proteins related to cell division were less abundant. The metaproteomic study revealed that the ETBE does have an effect on the metabolism of the bacterial consortium. It also enabled us to understand the responses of the complex bacterial consortium to ETBE, thus revealing interesting facts about the ETBE degrading bacterial community.https://www.mdpi.com/2076-2607/10/12/2331ETBEbiodegradationbacterial consortiummetaproteome2D-DIGE |
| spellingShingle | Vijayalakshmi Gunasekaran Núria Canela Magda Constantí Comparative Proteomic Analysis of an Ethyl Tert-Butyl Ether-Degrading Bacterial Consortium Microorganisms ETBE biodegradation bacterial consortium metaproteome 2D-DIGE |
| title | Comparative Proteomic Analysis of an Ethyl Tert-Butyl Ether-Degrading Bacterial Consortium |
| title_full | Comparative Proteomic Analysis of an Ethyl Tert-Butyl Ether-Degrading Bacterial Consortium |
| title_fullStr | Comparative Proteomic Analysis of an Ethyl Tert-Butyl Ether-Degrading Bacterial Consortium |
| title_full_unstemmed | Comparative Proteomic Analysis of an Ethyl Tert-Butyl Ether-Degrading Bacterial Consortium |
| title_short | Comparative Proteomic Analysis of an Ethyl Tert-Butyl Ether-Degrading Bacterial Consortium |
| title_sort | comparative proteomic analysis of an ethyl tert butyl ether degrading bacterial consortium |
| topic | ETBE biodegradation bacterial consortium metaproteome 2D-DIGE |
| url | https://www.mdpi.com/2076-2607/10/12/2331 |
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