Intraocular pressure reduction in a pigmentary glaucoma model by Goniotome Ab interno trabeculectomy.
<h4>Purpose</h4>To investigate whether microsurgical excision of trabecular meshwork (TM) in an ex vivo pigmentary glaucoma model can normalize the hypertensive phenotype.<h4>Methods</h4>Eight eyes of a porcine pigmentary glaucoma model underwent 90° of microsurgical TM excis...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0231360 |
| _version_ | 1818583893265088512 |
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| author | Chao Wang Yalong Dang Priyal Shah Hamed Esfandiari Ying Hong Ralitsa T Loewen Susannah Waxman Sarah Atta Xiaobo Xia Nils A Loewen |
| author_facet | Chao Wang Yalong Dang Priyal Shah Hamed Esfandiari Ying Hong Ralitsa T Loewen Susannah Waxman Sarah Atta Xiaobo Xia Nils A Loewen |
| author_sort | Chao Wang |
| collection | DOAJ |
| description | <h4>Purpose</h4>To investigate whether microsurgical excision of trabecular meshwork (TM) in an ex vivo pigmentary glaucoma model can normalize the hypertensive phenotype.<h4>Methods</h4>Eight eyes of a porcine pigmentary glaucoma model underwent 90° of microsurgical TM excision with an aspirating dual-blade (Goniotome (G)). 24 hours later, additional 90° of TM were removed. Anterior segments with sham surgeries served as the control (C). Outflow facility and intraocular pressure (IOP) were analyzed. Histology with hematoxylin and eosin (H&E) was obtained.<h4>Results</h4>After the first 90° TM excision, IOP was significantly lower in G (10.2±2.4 mmHg, n = 7) than C (20.0±2.0mmHg, n = 8, P<0.01). Outflow facility in G (0.38±0.07 μl/min/mmHg) was higher than C (0.16±0.02 μl/min/mmHg, P<0.01). After the second 90° TM excision, IOP in G (6.46±0.81 mmHg, n = 7) was significantly lower than C (20.3±1.7 mmHg, n = 8, P<0.001), while the outflow facility in G (0.50±0.05 μl/min/mmHg, n = 7) was higher than C (0.16±0.01 μl/min/mmHg, n = 8, P<0.001). Compared to the first excision, excision of an additional 90° did not change of IOP (P = 0.20) or outflow facility (P = 0.17) further.<h4>Conclusions</h4>Excision of 90° of TM in a pigmentary glaucoma model using an aspirating dual-blade decreased IOP and increased outflow facility. |
| first_indexed | 2024-12-16T08:12:31Z |
| format | Article |
| id | doaj.art-d776e03e9a674bcdbdd38d831116fac6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T08:12:31Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d776e03e9a674bcdbdd38d831116fac62022-12-21T22:38:20ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01154e023136010.1371/journal.pone.0231360Intraocular pressure reduction in a pigmentary glaucoma model by Goniotome Ab interno trabeculectomy.Chao WangYalong DangPriyal ShahHamed EsfandiariYing HongRalitsa T LoewenSusannah WaxmanSarah AttaXiaobo XiaNils A Loewen<h4>Purpose</h4>To investigate whether microsurgical excision of trabecular meshwork (TM) in an ex vivo pigmentary glaucoma model can normalize the hypertensive phenotype.<h4>Methods</h4>Eight eyes of a porcine pigmentary glaucoma model underwent 90° of microsurgical TM excision with an aspirating dual-blade (Goniotome (G)). 24 hours later, additional 90° of TM were removed. Anterior segments with sham surgeries served as the control (C). Outflow facility and intraocular pressure (IOP) were analyzed. Histology with hematoxylin and eosin (H&E) was obtained.<h4>Results</h4>After the first 90° TM excision, IOP was significantly lower in G (10.2±2.4 mmHg, n = 7) than C (20.0±2.0mmHg, n = 8, P<0.01). Outflow facility in G (0.38±0.07 μl/min/mmHg) was higher than C (0.16±0.02 μl/min/mmHg, P<0.01). After the second 90° TM excision, IOP in G (6.46±0.81 mmHg, n = 7) was significantly lower than C (20.3±1.7 mmHg, n = 8, P<0.001), while the outflow facility in G (0.50±0.05 μl/min/mmHg, n = 7) was higher than C (0.16±0.01 μl/min/mmHg, n = 8, P<0.001). Compared to the first excision, excision of an additional 90° did not change of IOP (P = 0.20) or outflow facility (P = 0.17) further.<h4>Conclusions</h4>Excision of 90° of TM in a pigmentary glaucoma model using an aspirating dual-blade decreased IOP and increased outflow facility.https://doi.org/10.1371/journal.pone.0231360 |
| spellingShingle | Chao Wang Yalong Dang Priyal Shah Hamed Esfandiari Ying Hong Ralitsa T Loewen Susannah Waxman Sarah Atta Xiaobo Xia Nils A Loewen Intraocular pressure reduction in a pigmentary glaucoma model by Goniotome Ab interno trabeculectomy. PLoS ONE |
| title | Intraocular pressure reduction in a pigmentary glaucoma model by Goniotome Ab interno trabeculectomy. |
| title_full | Intraocular pressure reduction in a pigmentary glaucoma model by Goniotome Ab interno trabeculectomy. |
| title_fullStr | Intraocular pressure reduction in a pigmentary glaucoma model by Goniotome Ab interno trabeculectomy. |
| title_full_unstemmed | Intraocular pressure reduction in a pigmentary glaucoma model by Goniotome Ab interno trabeculectomy. |
| title_short | Intraocular pressure reduction in a pigmentary glaucoma model by Goniotome Ab interno trabeculectomy. |
| title_sort | intraocular pressure reduction in a pigmentary glaucoma model by goniotome ab interno trabeculectomy |
| url | https://doi.org/10.1371/journal.pone.0231360 |
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