Calycosin-loaded nanoliposomes as potential nanoplatforms for treatment of diabetic nephropathy through regulation of mitochondrial respiratory function
Abstract Backgrounds One of the most common complications in diabetic nephropathy is generation of high levels of ROS which can be regulated by herbal antioxidants. However, polyphenols like calycosin, the bioactive compound of Radix astragali suffer from low solubility and poor bioavailability. Met...
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| Format: | Article |
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BMC
2021-06-01
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| Series: | Journal of Nanobiotechnology |
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| Online Access: | https://doi.org/10.1186/s12951-021-00917-1 |
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| author | Chunrong Huang Lian-Fang Xue Bo Hu Huan-Huan Liu Si-Bo Huang Suliman Khan Yu Meng |
| author_facet | Chunrong Huang Lian-Fang Xue Bo Hu Huan-Huan Liu Si-Bo Huang Suliman Khan Yu Meng |
| author_sort | Chunrong Huang |
| collection | DOAJ |
| description | Abstract Backgrounds One of the most common complications in diabetic nephropathy is generation of high levels of ROS which can be regulated by herbal antioxidants. However, polyphenols like calycosin, the bioactive compound of Radix astragali suffer from low solubility and poor bioavailability. Methods Therefore, in the present study, calycosin-loaded nanoliposomes were fabricated and characterized by TEM, DLS and FTIR techniques. Afterwards, the drug loading (DL) and entrapment efficiency (EE), drug release, solubility, stability, and pharmacodynamic assays were performed. Finally, the antinephropathic effects of calycosin-loaded-nanoliposomes on mitochondria of kidney cells were explored by MTT, ROS, MDA, mitochondrial respiratory function assays. Results The result showed that the size, hydrodynamic radius, zeta potential, EE, and DL were, 80 nm, 133.99 ± 21.44 nm, − 20.53 ± 3.57, 88.37 ± 2.28%, and 7.48 ± 1.19%, respectively. The outcomes of in vitro release assay showed that calycosin-loaded nanoliposomes were significantly slow-release in dialysis media with pH 1.2, pH 6.9 and pH 7.4, at about 30 min, the dissolution of calycosin from nanoliposome became almost complete, and after 2 months, the calycosin-loaded nanoliposomes were still stable. Pharmacokinetic assay revealed that the AUC0−t of calycosin in calycosin-loaded nanoliposome group was 927.39 ± 124.91 μg/L*h, which was 2.26 times than that of the free calycosin group (**P < 0.01). Additionally, the MRT0−t and t1/2 of calycosin in the calycosin-loaded nanoliposome group were prolonged by 1.54 times and 1.33 times than that of free calycosin group, respectively (*P < 0.05). Finally, it was shown that calycosin-loaded nanoliposomes regulated the viability, ROS production, lipid peroxidation and function of mitochondria in kidney cells of diabetic rats as a model of diabetic nephropathy. Conclusion In conclusion it may be suggested that new therapies based on nano-formulated calycosin can restore mitochondrial function which can improve diabetic nephropathy. Graphic abstract |
| first_indexed | 2024-04-14T03:29:29Z |
| format | Article |
| id | doaj.art-d77b9cad2cee4ea0b573577e6fcc7516 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-14T03:29:29Z |
| publishDate | 2021-06-01 |
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| series | Journal of Nanobiotechnology |
| spelling | doaj.art-d77b9cad2cee4ea0b573577e6fcc75162022-12-22T02:15:00ZengBMCJournal of Nanobiotechnology1477-31552021-06-0119111210.1186/s12951-021-00917-1Calycosin-loaded nanoliposomes as potential nanoplatforms for treatment of diabetic nephropathy through regulation of mitochondrial respiratory functionChunrong Huang0Lian-Fang Xue1Bo Hu2Huan-Huan Liu3Si-Bo Huang4Suliman Khan5Yu Meng6Department of Gastroenterology, The First Hospital Affiliated To Jinan UniversityDepartment of Clinical Pharmacy, The First Affiliated Hospital of Jinan UniversityDepartment of Nephrology, The First Hospital Affiliated To Jinan UniversityDepartment of Nephrology, The First Hospital Affiliated To Jinan UniversityDepartment of Nephrology, The First Hospital Affiliated To Jinan UniversityDepartment of Cerebrovascular Diseases, The Second Affiliated Hospital of Zhengzhou UniversityDepartment of Nephrology, The First Hospital Affiliated To Jinan UniversityAbstract Backgrounds One of the most common complications in diabetic nephropathy is generation of high levels of ROS which can be regulated by herbal antioxidants. However, polyphenols like calycosin, the bioactive compound of Radix astragali suffer from low solubility and poor bioavailability. Methods Therefore, in the present study, calycosin-loaded nanoliposomes were fabricated and characterized by TEM, DLS and FTIR techniques. Afterwards, the drug loading (DL) and entrapment efficiency (EE), drug release, solubility, stability, and pharmacodynamic assays were performed. Finally, the antinephropathic effects of calycosin-loaded-nanoliposomes on mitochondria of kidney cells were explored by MTT, ROS, MDA, mitochondrial respiratory function assays. Results The result showed that the size, hydrodynamic radius, zeta potential, EE, and DL were, 80 nm, 133.99 ± 21.44 nm, − 20.53 ± 3.57, 88.37 ± 2.28%, and 7.48 ± 1.19%, respectively. The outcomes of in vitro release assay showed that calycosin-loaded nanoliposomes were significantly slow-release in dialysis media with pH 1.2, pH 6.9 and pH 7.4, at about 30 min, the dissolution of calycosin from nanoliposome became almost complete, and after 2 months, the calycosin-loaded nanoliposomes were still stable. Pharmacokinetic assay revealed that the AUC0−t of calycosin in calycosin-loaded nanoliposome group was 927.39 ± 124.91 μg/L*h, which was 2.26 times than that of the free calycosin group (**P < 0.01). Additionally, the MRT0−t and t1/2 of calycosin in the calycosin-loaded nanoliposome group were prolonged by 1.54 times and 1.33 times than that of free calycosin group, respectively (*P < 0.05). Finally, it was shown that calycosin-loaded nanoliposomes regulated the viability, ROS production, lipid peroxidation and function of mitochondria in kidney cells of diabetic rats as a model of diabetic nephropathy. Conclusion In conclusion it may be suggested that new therapies based on nano-formulated calycosin can restore mitochondrial function which can improve diabetic nephropathy. Graphic abstracthttps://doi.org/10.1186/s12951-021-00917-1Diabetic nephropathyROSCalycosinNanoliposomeMitochondria |
| spellingShingle | Chunrong Huang Lian-Fang Xue Bo Hu Huan-Huan Liu Si-Bo Huang Suliman Khan Yu Meng Calycosin-loaded nanoliposomes as potential nanoplatforms for treatment of diabetic nephropathy through regulation of mitochondrial respiratory function Journal of Nanobiotechnology Diabetic nephropathy ROS Calycosin Nanoliposome Mitochondria |
| title | Calycosin-loaded nanoliposomes as potential nanoplatforms for treatment of diabetic nephropathy through regulation of mitochondrial respiratory function |
| title_full | Calycosin-loaded nanoliposomes as potential nanoplatforms for treatment of diabetic nephropathy through regulation of mitochondrial respiratory function |
| title_fullStr | Calycosin-loaded nanoliposomes as potential nanoplatforms for treatment of diabetic nephropathy through regulation of mitochondrial respiratory function |
| title_full_unstemmed | Calycosin-loaded nanoliposomes as potential nanoplatforms for treatment of diabetic nephropathy through regulation of mitochondrial respiratory function |
| title_short | Calycosin-loaded nanoliposomes as potential nanoplatforms for treatment of diabetic nephropathy through regulation of mitochondrial respiratory function |
| title_sort | calycosin loaded nanoliposomes as potential nanoplatforms for treatment of diabetic nephropathy through regulation of mitochondrial respiratory function |
| topic | Diabetic nephropathy ROS Calycosin Nanoliposome Mitochondria |
| url | https://doi.org/10.1186/s12951-021-00917-1 |
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