Male Selenoprotein F-Like (SPF-L) Influences Female Reproduction and Population Growth in Nilaparvata lugens (Hemiptera: Delphacidae)
Selenoproteins serve in anti-oxidant and cellular redox functions in almost all organisms. A recent study characterized a selenoprotein F-like (SPF-L) in the brown plant hopper’s (BPH), Nilaparvata lugens, male accessory glands (MAGs), raised the question of whether the SPF-L is associated with fema...
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| Format: | Article |
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Frontiers Media S.A.
2019-09-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2019.01196/full |
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| author | LinQuan Ge YongKai Zhou HaoTian Gu Qing Wu Ze Zhou Sui Zheng David Stanley QiSheng Song |
| author_facet | LinQuan Ge YongKai Zhou HaoTian Gu Qing Wu Ze Zhou Sui Zheng David Stanley QiSheng Song |
| author_sort | LinQuan Ge |
| collection | DOAJ |
| description | Selenoproteins serve in anti-oxidant and cellular redox functions in almost all organisms. A recent study characterized a selenoprotein F-like (SPF-L) in the brown plant hopper’s (BPH), Nilaparvata lugens, male accessory glands (MAGs), raised the question of whether the SPF-L is associated with female fecundity. In this study, SPF-L mRNA was found to be enriched in the internal reproductive organ (IRO) of virgin males, also expressed relatively stably in virgin males and females, and dietary dsSPF-L-treatments led to reduced MAG protein and Arginine content. Knockdown of NlSPF-L in unmated males did not influence juvenile hormone (JH) III and ecdysteroid titers, however, dsSPF-L-treated mated males had increased JH III titer, and reduced ecdysteroid titer compared to controls. After mating with dsSPF-L-treated males, female partners had reduced fat body and ovary soluble proteins and JH III tier and vitellogenin (Vg) mRNA levels, but no alterations in ecdysteroid titer, body weight or longevity. The experimental females had prolonged pre-oviposition periods and they laid fewer eggs, which suffered reduced hatching rates and population growth index (PGI). Such mating also led to impaired IRO development in males and females, which was confirmed by immunofluorescence staining. We infer that SPF-L affects reproductive success of males and their partners. |
| first_indexed | 2024-12-19T05:52:47Z |
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| id | doaj.art-d77c27961d7449c2a2b35156e59b5a3d |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-19T05:52:47Z |
| publishDate | 2019-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-d77c27961d7449c2a2b35156e59b5a3d2022-12-21T20:33:32ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2019-09-011010.3389/fphys.2019.01196480174Male Selenoprotein F-Like (SPF-L) Influences Female Reproduction and Population Growth in Nilaparvata lugens (Hemiptera: Delphacidae)LinQuan Ge0YongKai Zhou1HaoTian Gu2Qing Wu3Ze Zhou4Sui Zheng5David Stanley6QiSheng Song7School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, ChinaSchool of Horticulture and Plant Protection, Yangzhou University, Yangzhou, ChinaSchool of Horticulture and Plant Protection, Yangzhou University, Yangzhou, ChinaSchool of Horticulture and Plant Protection, Yangzhou University, Yangzhou, ChinaSchool of Horticulture and Plant Protection, Yangzhou University, Yangzhou, ChinaSchool of Horticulture and Plant Protection, Yangzhou University, Yangzhou, ChinaBiological Control of Insects Research Laboratory, USDA/Agricultural Research Service, Columbia, MO, United StatesDivision of Plant Sciences, University of Missouri, Columbia, MO, United StatesSelenoproteins serve in anti-oxidant and cellular redox functions in almost all organisms. A recent study characterized a selenoprotein F-like (SPF-L) in the brown plant hopper’s (BPH), Nilaparvata lugens, male accessory glands (MAGs), raised the question of whether the SPF-L is associated with female fecundity. In this study, SPF-L mRNA was found to be enriched in the internal reproductive organ (IRO) of virgin males, also expressed relatively stably in virgin males and females, and dietary dsSPF-L-treatments led to reduced MAG protein and Arginine content. Knockdown of NlSPF-L in unmated males did not influence juvenile hormone (JH) III and ecdysteroid titers, however, dsSPF-L-treated mated males had increased JH III titer, and reduced ecdysteroid titer compared to controls. After mating with dsSPF-L-treated males, female partners had reduced fat body and ovary soluble proteins and JH III tier and vitellogenin (Vg) mRNA levels, but no alterations in ecdysteroid titer, body weight or longevity. The experimental females had prolonged pre-oviposition periods and they laid fewer eggs, which suffered reduced hatching rates and population growth index (PGI). Such mating also led to impaired IRO development in males and females, which was confirmed by immunofluorescence staining. We infer that SPF-L affects reproductive success of males and their partners.https://www.frontiersin.org/article/10.3389/fphys.2019.01196/fullNilaparvata lugensselenoproteinseminal fluidsmatingfecundity |
| spellingShingle | LinQuan Ge YongKai Zhou HaoTian Gu Qing Wu Ze Zhou Sui Zheng David Stanley QiSheng Song Male Selenoprotein F-Like (SPF-L) Influences Female Reproduction and Population Growth in Nilaparvata lugens (Hemiptera: Delphacidae) Frontiers in Physiology Nilaparvata lugens selenoprotein seminal fluids mating fecundity |
| title | Male Selenoprotein F-Like (SPF-L) Influences Female Reproduction and Population Growth in Nilaparvata lugens (Hemiptera: Delphacidae) |
| title_full | Male Selenoprotein F-Like (SPF-L) Influences Female Reproduction and Population Growth in Nilaparvata lugens (Hemiptera: Delphacidae) |
| title_fullStr | Male Selenoprotein F-Like (SPF-L) Influences Female Reproduction and Population Growth in Nilaparvata lugens (Hemiptera: Delphacidae) |
| title_full_unstemmed | Male Selenoprotein F-Like (SPF-L) Influences Female Reproduction and Population Growth in Nilaparvata lugens (Hemiptera: Delphacidae) |
| title_short | Male Selenoprotein F-Like (SPF-L) Influences Female Reproduction and Population Growth in Nilaparvata lugens (Hemiptera: Delphacidae) |
| title_sort | male selenoprotein f like spf l influences female reproduction and population growth in nilaparvata lugens hemiptera delphacidae |
| topic | Nilaparvata lugens selenoprotein seminal fluids mating fecundity |
| url | https://www.frontiersin.org/article/10.3389/fphys.2019.01196/full |
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