#264 : 3D Time-Lapse Refractive-Index Imaging of Intact Mouse Embryos for Non-Invasive Evaluation of the Embryonic Development
Background and Aims: Time-lapse technology is an emerging assisted reproductive technology (ART) that enables simultaneous culture and monitoring of embryos in vitro during their development. It provides extra information on key events during embryo development, which can assist embryologists in sel...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
World Scientific Publishing
2023-12-01
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| Series: | Fertility & Reproduction |
| Online Access: | https://www.worldscientific.com/doi/10.1142/S2661318223742133 |
| _version_ | 1797237024737984512 |
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| author | Chungha Lee Sangho Lee Taeseop Shin Geon Kim Jieun Do Jaephil Do Ji Hyang Kim Kyoung Hee Choi Jae Young Kim YongKeun Park |
| author_facet | Chungha Lee Sangho Lee Taeseop Shin Geon Kim Jieun Do Jaephil Do Ji Hyang Kim Kyoung Hee Choi Jae Young Kim YongKeun Park |
| author_sort | Chungha Lee |
| collection | DOAJ |
| description | Background and Aims: Time-lapse technology is an emerging assisted reproductive technology (ART) that enables simultaneous culture and monitoring of embryos in vitro during their development. It provides extra information on key events during embryo development, which can assist embryologists in selecting embryos alongside traditional morphology evaluations. Various imaging technologies have been utilised to visualise and evaluate embryos such as brightfield microscopy, darkfield microscopy, and Hoffman modulation contrast microscopy. Although these imaging technologies allow for non-invasive imaging of human embryos in a clinical setting, 3D imaging of a whole embryo with subcellular resolution remains a challenge. Method: We demonstrate 3D time-lapse label-free imaging of live mouse embryos using the 3D refractive index (RI) imaging technique. The proposed method is a quantitative phase imaging technique that measures multiple-intensity images of transmitted light through the embryos via axial scanning to reconstruct the 3D RI map of the embryos. Results: Using the proposed method, live mouse embryos were monitored at different developmental stages, ranging from 2-cell embryos to expanded blastocysts. The measured 3D RI maps of the developing embryos reveal 3D landscape of subcellular features inside the embryos (Fig. 1). In the quantitative assessment of embryonic development, various morphokinematic parameters were extracted from the measured 3D RI maps of the embryos. Conclusion: Based on the results, we envision that our method has broad applications where non-invasive and quantitative assessment of embryonic development is required. |
| first_indexed | 2024-04-24T17:13:10Z |
| format | Article |
| id | doaj.art-d781f939a05f4bf1945ec3c0d78ef240 |
| institution | Directory Open Access Journal |
| issn | 2661-3182 2661-3174 |
| language | English |
| last_indexed | 2024-04-24T17:13:10Z |
| publishDate | 2023-12-01 |
| publisher | World Scientific Publishing |
| record_format | Article |
| series | Fertility & Reproduction |
| spelling | doaj.art-d781f939a05f4bf1945ec3c0d78ef2402024-03-28T07:54:19ZengWorld Scientific PublishingFertility & Reproduction2661-31822661-31742023-12-01050443043110.1142/S2661318223742133#264 : 3D Time-Lapse Refractive-Index Imaging of Intact Mouse Embryos for Non-Invasive Evaluation of the Embryonic DevelopmentChungha Lee0Sangho Lee1Taeseop Shin2Geon Kim3Jieun Do4Jaephil Do5Ji Hyang Kim6Kyoung Hee Choi7Jae Young Kim8YongKeun Park9Korea Advanced Institute of Science And Technology (KAIST), Daejeon, South KoreaFertility Center of CHA Bundang Women’s Medical Center, Seongnam, South KoreaFertility Center of CHA Bundang Women’s Medical Center, Seongnam, South KoreaKorea Advanced Institute of Science And Technology (KAIST), Daejeon, South KoreaKAIST Laboratory Animal Resource Center, Daejeon, South KoreaTomocube Inc., Daejeon, South KoreaFertility Center of CHA Bundang Women’s Medical Center, Seongnam, South KoreaFertility Center of CHA Bundang Women’s Medical Center, Seongnam, South KoreaFertility Center of CHA Bundang Women’s Medical Center, Seongnam, South KoreaKorea Advanced Institute of Science And Technology (KAIST), Daejeon, South KoreaBackground and Aims: Time-lapse technology is an emerging assisted reproductive technology (ART) that enables simultaneous culture and monitoring of embryos in vitro during their development. It provides extra information on key events during embryo development, which can assist embryologists in selecting embryos alongside traditional morphology evaluations. Various imaging technologies have been utilised to visualise and evaluate embryos such as brightfield microscopy, darkfield microscopy, and Hoffman modulation contrast microscopy. Although these imaging technologies allow for non-invasive imaging of human embryos in a clinical setting, 3D imaging of a whole embryo with subcellular resolution remains a challenge. Method: We demonstrate 3D time-lapse label-free imaging of live mouse embryos using the 3D refractive index (RI) imaging technique. The proposed method is a quantitative phase imaging technique that measures multiple-intensity images of transmitted light through the embryos via axial scanning to reconstruct the 3D RI map of the embryos. Results: Using the proposed method, live mouse embryos were monitored at different developmental stages, ranging from 2-cell embryos to expanded blastocysts. The measured 3D RI maps of the developing embryos reveal 3D landscape of subcellular features inside the embryos (Fig. 1). In the quantitative assessment of embryonic development, various morphokinematic parameters were extracted from the measured 3D RI maps of the embryos. Conclusion: Based on the results, we envision that our method has broad applications where non-invasive and quantitative assessment of embryonic development is required.https://www.worldscientific.com/doi/10.1142/S2661318223742133 |
| spellingShingle | Chungha Lee Sangho Lee Taeseop Shin Geon Kim Jieun Do Jaephil Do Ji Hyang Kim Kyoung Hee Choi Jae Young Kim YongKeun Park #264 : 3D Time-Lapse Refractive-Index Imaging of Intact Mouse Embryos for Non-Invasive Evaluation of the Embryonic Development Fertility & Reproduction |
| title | #264 : 3D Time-Lapse Refractive-Index Imaging of Intact Mouse Embryos for Non-Invasive Evaluation of the Embryonic Development |
| title_full | #264 : 3D Time-Lapse Refractive-Index Imaging of Intact Mouse Embryos for Non-Invasive Evaluation of the Embryonic Development |
| title_fullStr | #264 : 3D Time-Lapse Refractive-Index Imaging of Intact Mouse Embryos for Non-Invasive Evaluation of the Embryonic Development |
| title_full_unstemmed | #264 : 3D Time-Lapse Refractive-Index Imaging of Intact Mouse Embryos for Non-Invasive Evaluation of the Embryonic Development |
| title_short | #264 : 3D Time-Lapse Refractive-Index Imaging of Intact Mouse Embryos for Non-Invasive Evaluation of the Embryonic Development |
| title_sort | 264 3d time lapse refractive index imaging of intact mouse embryos for non invasive evaluation of the embryonic development |
| url | https://www.worldscientific.com/doi/10.1142/S2661318223742133 |
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