| Summary: | Replacement of fish oil by 5% thraustochytrid whole cell biomass in diets for Atlantic salmon had no ill effect on fish growth performance, carcass total lipid and total fatty acid content. Carcass fatty acid composition indicated incorporation of the dietary thraustochytrid-derived fatty acids. This was confirmed by compound specific stable isotope analysis (CSIA) which revealed significantly <sup>13</sup>C-depleted (δ<sup>13</sup>C value of −24‱) ω3 long-chain (≥C<sub>20</sub>) polyunsaturated fatty acids (ω3 LC-PUFAs) in the fingerlings fed the thraustochytrid biomass containing diet, reflecting the highly <sup>13</sup>C-depleted glycerol used to grow the thraustochytrid cultures. This finding demonstrates the bioavailability of the ω3 LC-PUFA of the Australian strain thraustochytrid culture (TC) 20 from the whole cell biomass that was partly cultivated on crude glycerol produced during biodiesel manufacturing. This paper demonstrates the value of Australian thraustochytrid strains grown heterotrophically for their wider biotechnological potential including as a source of higher value lipids, in particular the health-benefitting ω3 LC-PUFA, for use in aquaculture and other applications.
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