Tribological Maturation of Regenerated Cartilage was Inhibited by Using Chondrocyte Aggregates
Cartilage tissue was regenerated using fibroin sponge seeded with chondrocyte aggregates. The aggregates were formed on the sponge by micro-folding culture, where chondrocytes were cultured on a substrate containing an array of pits of 100 μm diameter. The aggregate-derived cartilage tissue s...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The Japan Society of Mechanical Engineers
2009-05-01
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| Series: | Journal of Biomechanical Science and Engineering |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jbse/4/2/4_2_174/_pdf/-char/en |
| _version_ | 1828192508558966784 |
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| author | Naoyoshi KACHI Naohide TOMITA Koji YAMAMOTO Riki TAKAYA Yasushi TAMADA |
| author_facet | Naoyoshi KACHI Naohide TOMITA Koji YAMAMOTO Riki TAKAYA Yasushi TAMADA |
| author_sort | Naoyoshi KACHI |
| collection | DOAJ |
| description | Cartilage tissue was regenerated using fibroin sponge seeded with chondrocyte aggregates. The aggregates were formed on the sponge by micro-folding culture, where chondrocytes were cultured on a substrate containing an array of pits of 100 μm diameter. The aggregate-derived cartilage tissue showed higher safranin O staining intensity and was positive for collagen type II by immunostaining. However, its friction coefficient was higher than that of the cartilage tissue regenerated using individual cells. Moreover, the surface of this aggregate-derived cartilage tissue showed weak positive staining for collagen type I. Thus, regeneration of the aggregate-derived cartilage tissue has the possibility of forming larger amounts of extracellular matrix from a limited number of chondrocytes. However, such tissue showed relatively poor tribological function possibly because of its immature functional layer structure. |
| first_indexed | 2024-04-12T08:55:25Z |
| format | Article |
| id | doaj.art-d787d919171341a899f5820789097ea6 |
| institution | Directory Open Access Journal |
| issn | 1880-9863 |
| language | English |
| last_indexed | 2024-04-12T08:55:25Z |
| publishDate | 2009-05-01 |
| publisher | The Japan Society of Mechanical Engineers |
| record_format | Article |
| series | Journal of Biomechanical Science and Engineering |
| spelling | doaj.art-d787d919171341a899f5820789097ea62022-12-22T03:39:24ZengThe Japan Society of Mechanical EngineersJournal of Biomechanical Science and Engineering1880-98632009-05-014217418110.1299/jbse.4.174jbseTribological Maturation of Regenerated Cartilage was Inhibited by Using Chondrocyte AggregatesNaoyoshi KACHI0Naohide TOMITA1Koji YAMAMOTO2Riki TAKAYA3Yasushi TAMADA4Department of Mechanical Engineering and Science, Graduate School of Engineering, Kyoto UniversityDepartment of Mechanical Engineering and Science, Graduate School of Engineering, Kyoto UniversityDepartment of Mechanical Engineering and Science, Graduate School of Engineering, Kyoto UniversityDepartment of Mechanical Engineering and Science, Graduate School of Engineering, Kyoto UniversityNational Institute of Agrobiological SciencesCartilage tissue was regenerated using fibroin sponge seeded with chondrocyte aggregates. The aggregates were formed on the sponge by micro-folding culture, where chondrocytes were cultured on a substrate containing an array of pits of 100 μm diameter. The aggregate-derived cartilage tissue showed higher safranin O staining intensity and was positive for collagen type II by immunostaining. However, its friction coefficient was higher than that of the cartilage tissue regenerated using individual cells. Moreover, the surface of this aggregate-derived cartilage tissue showed weak positive staining for collagen type I. Thus, regeneration of the aggregate-derived cartilage tissue has the possibility of forming larger amounts of extracellular matrix from a limited number of chondrocytes. However, such tissue showed relatively poor tribological function possibly because of its immature functional layer structure.https://www.jstage.jst.go.jp/article/jbse/4/2/4_2_174/_pdf/-char/enfibroin spongecartilagechondrocytecell aggregatefriction test |
| spellingShingle | Naoyoshi KACHI Naohide TOMITA Koji YAMAMOTO Riki TAKAYA Yasushi TAMADA Tribological Maturation of Regenerated Cartilage was Inhibited by Using Chondrocyte Aggregates Journal of Biomechanical Science and Engineering fibroin sponge cartilage chondrocyte cell aggregate friction test |
| title | Tribological Maturation of Regenerated Cartilage was Inhibited by Using Chondrocyte Aggregates |
| title_full | Tribological Maturation of Regenerated Cartilage was Inhibited by Using Chondrocyte Aggregates |
| title_fullStr | Tribological Maturation of Regenerated Cartilage was Inhibited by Using Chondrocyte Aggregates |
| title_full_unstemmed | Tribological Maturation of Regenerated Cartilage was Inhibited by Using Chondrocyte Aggregates |
| title_short | Tribological Maturation of Regenerated Cartilage was Inhibited by Using Chondrocyte Aggregates |
| title_sort | tribological maturation of regenerated cartilage was inhibited by using chondrocyte aggregates |
| topic | fibroin sponge cartilage chondrocyte cell aggregate friction test |
| url | https://www.jstage.jst.go.jp/article/jbse/4/2/4_2_174/_pdf/-char/en |
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