Cardiomyopathy in offspring of diabetic rats is associated with activation of the MAPK and apoptotic pathways
<p>Abstract</p> <p>Background</p> <p>Maternal diabetes affects the developing fetal cardiovascular system. Newborn offspring of diabetic mothers can have a transient cardiomyopathy. We hypothesized that cardiomyopathic remodeling is associated with activation of the mit...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2009-07-01
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| Series: | Cardiovascular Diabetology |
| Online Access: | http://www.cardiab.com/content/8/1/43 |
| _version_ | 1819089069650477056 |
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| author | Segar Jeffrey L Weiss Robert M Wedemeyer Elesa W Reinking Benjamin E Scholz Thomas D |
| author_facet | Segar Jeffrey L Weiss Robert M Wedemeyer Elesa W Reinking Benjamin E Scholz Thomas D |
| author_sort | Segar Jeffrey L |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Maternal diabetes affects the developing fetal cardiovascular system. Newborn offspring of diabetic mothers can have a transient cardiomyopathy. We hypothesized that cardiomyopathic remodeling is associated with activation of the mitogen activated protein kinase (MAPK) signaling and apoptotic pathways.</p> <p>Methods</p> <p>To evaluate the effects of moderate and severe maternal hyperglycemia, pregnant rats were made diabetic with an injection of 50 mg/kg of streptozotocin. Moderately well controlled maternal diabetes was achieved with twice daily glucose checks and insulin injections. No insulin was given to severely diabetic dams. Offspring of moderate and severe diabetic mothers (OMDM and MSDM, respectively) were studied on postnatal days 1 (NB1) and 21 (NB21). Echocardiograms were performed to evaluate left ventricular (LV) dimensions and function. Myocardial MAPK and apoptotic protein levels were measured by Western blot.</p> <p>Results</p> <p>OMDM had increased cardiac mass at NB1 compared to controls that normalized at NB21. OSDM demonstrated microsomia with relative sparing of cardiac mass and a dilated cardiomyopathy at NB1. In both models, there was a persistent increase in the HW:BW and significant activation of MAPK and apoptotic pathways at NB21.</p> <p>Conclusion</p> <p>The degree of maternal hyperglycemia determines the type of cardiomyopathy seen in the offspring, while resolution of both the hypertrophic and dilated cardiomyopathies is associated with activation of MAPK signaling and apoptotic pathways.</p> |
| first_indexed | 2024-12-21T22:02:04Z |
| format | Article |
| id | doaj.art-d7885dd0e61445c5ae6a49ef5b676d29 |
| institution | Directory Open Access Journal |
| issn | 1475-2840 |
| language | English |
| last_indexed | 2024-12-21T22:02:04Z |
| publishDate | 2009-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Cardiovascular Diabetology |
| spelling | doaj.art-d7885dd0e61445c5ae6a49ef5b676d292022-12-21T18:48:48ZengBMCCardiovascular Diabetology1475-28402009-07-01814310.1186/1475-2840-8-43Cardiomyopathy in offspring of diabetic rats is associated with activation of the MAPK and apoptotic pathwaysSegar Jeffrey LWeiss Robert MWedemeyer Elesa WReinking Benjamin EScholz Thomas D<p>Abstract</p> <p>Background</p> <p>Maternal diabetes affects the developing fetal cardiovascular system. Newborn offspring of diabetic mothers can have a transient cardiomyopathy. We hypothesized that cardiomyopathic remodeling is associated with activation of the mitogen activated protein kinase (MAPK) signaling and apoptotic pathways.</p> <p>Methods</p> <p>To evaluate the effects of moderate and severe maternal hyperglycemia, pregnant rats were made diabetic with an injection of 50 mg/kg of streptozotocin. Moderately well controlled maternal diabetes was achieved with twice daily glucose checks and insulin injections. No insulin was given to severely diabetic dams. Offspring of moderate and severe diabetic mothers (OMDM and MSDM, respectively) were studied on postnatal days 1 (NB1) and 21 (NB21). Echocardiograms were performed to evaluate left ventricular (LV) dimensions and function. Myocardial MAPK and apoptotic protein levels were measured by Western blot.</p> <p>Results</p> <p>OMDM had increased cardiac mass at NB1 compared to controls that normalized at NB21. OSDM demonstrated microsomia with relative sparing of cardiac mass and a dilated cardiomyopathy at NB1. In both models, there was a persistent increase in the HW:BW and significant activation of MAPK and apoptotic pathways at NB21.</p> <p>Conclusion</p> <p>The degree of maternal hyperglycemia determines the type of cardiomyopathy seen in the offspring, while resolution of both the hypertrophic and dilated cardiomyopathies is associated with activation of MAPK signaling and apoptotic pathways.</p>http://www.cardiab.com/content/8/1/43 |
| spellingShingle | Segar Jeffrey L Weiss Robert M Wedemeyer Elesa W Reinking Benjamin E Scholz Thomas D Cardiomyopathy in offspring of diabetic rats is associated with activation of the MAPK and apoptotic pathways Cardiovascular Diabetology |
| title | Cardiomyopathy in offspring of diabetic rats is associated with activation of the MAPK and apoptotic pathways |
| title_full | Cardiomyopathy in offspring of diabetic rats is associated with activation of the MAPK and apoptotic pathways |
| title_fullStr | Cardiomyopathy in offspring of diabetic rats is associated with activation of the MAPK and apoptotic pathways |
| title_full_unstemmed | Cardiomyopathy in offspring of diabetic rats is associated with activation of the MAPK and apoptotic pathways |
| title_short | Cardiomyopathy in offspring of diabetic rats is associated with activation of the MAPK and apoptotic pathways |
| title_sort | cardiomyopathy in offspring of diabetic rats is associated with activation of the mapk and apoptotic pathways |
| url | http://www.cardiab.com/content/8/1/43 |
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