Seselin promotes cisplatin-induced apoptosis of AGS gastric cancer cells by inhibiting β-catenin expression
Gastric cancer is a commonly diagnosed form of cancer, and cisplatin is commonly used as a chemotherapy drug for treating it. However, the side effects of cisplatin may reduce patients’ willingness to use it. Seselin, a derivative of coumarin, has been found to have anticancer properties as well as...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2023-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | https://doiserbia.nb.rs/img/doi/0354-4664/2023/0354-46642300023S.pdf |
| _version_ | 1797393764495392768 |
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| author | Shyu Rong-Yaun Wang Chun-Hua Wu Chang-Chieh Wang Lu-Kai Tsai Fu-Ming |
| author_facet | Shyu Rong-Yaun Wang Chun-Hua Wu Chang-Chieh Wang Lu-Kai Tsai Fu-Ming |
| author_sort | Shyu Rong-Yaun |
| collection | DOAJ |
| description | Gastric cancer is a commonly diagnosed form of cancer, and cisplatin is commonly used as a chemotherapy drug for treating it. However, the side effects of cisplatin may reduce patients’ willingness to use it. Seselin, a derivative of coumarin, has been found to have anticancer properties as well as anticoagulant effects. In this study, we investigated the effect of seselin on promoting cisplatin-induced gastric cancer cell death using the cell proliferation reagent WST-1, BrdU incorporation and lactate dehydrogenase release. The role of seselin and cisplatin in the apoptosis of gastric cancer cells was analyzed using a phospho-kinase array and Western blot analysis. Seselin did not affect G2/M stasis, but it promoted cell death in AGS cells treated with cisplatin. Phospho-kinase array analysis revealed that cisplatin regulates intracellular p53 phosphorylation, while seselin regulates intracellular β-catenin expression by affecting the phosphorylation of glycogen synthase kinase-3 beta (GSK-3β), extracellular-signal-regulated kinase (ERK) and Src tyrosine kinase. Seselin and cisplatin promote the apoptosis of gastric cancer cells by the synergistic effect of two distinct signaling pathways. These findings suggest that seselin may be used as a complementary therapy to reduce the clinical dose of chemotherapy. |
| first_indexed | 2024-03-09T00:07:34Z |
| format | Article |
| id | doaj.art-d78acc3f15d04c6680137cd34ddc3fba |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-03-09T00:07:34Z |
| publishDate | 2023-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-d78acc3f15d04c6680137cd34ddc3fba2023-12-12T13:06:47ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392023-01-0175328729710.2298/ABS230314023S0354-46642300023SSeselin promotes cisplatin-induced apoptosis of AGS gastric cancer cells by inhibiting β-catenin expressionShyu Rong-Yaun0Wang Chun-Hua1Wu Chang-Chieh2Wang Lu-Kai3Tsai Fu-Ming4Department of Internal Medicine, Taipei Tzu Chi Hospital, The Buddhist Tzu Chi Medical Foundation, New Taipei City, TaiwanDepartment of Dermatology, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, New Taipei City, Taiwan + School of Medicine, Tzu Chi University, Hualien, TaiwanDepartment of Surgery, Tri-Service General Hospital Keelung Branch, National Defense Medical Center, Keelung, TaiwanDepartment of Life Sciences, National Science and Technology Council, Taipei, TaiwanDepartment of Research, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, New Taipei City, TaiwanGastric cancer is a commonly diagnosed form of cancer, and cisplatin is commonly used as a chemotherapy drug for treating it. However, the side effects of cisplatin may reduce patients’ willingness to use it. Seselin, a derivative of coumarin, has been found to have anticancer properties as well as anticoagulant effects. In this study, we investigated the effect of seselin on promoting cisplatin-induced gastric cancer cell death using the cell proliferation reagent WST-1, BrdU incorporation and lactate dehydrogenase release. The role of seselin and cisplatin in the apoptosis of gastric cancer cells was analyzed using a phospho-kinase array and Western blot analysis. Seselin did not affect G2/M stasis, but it promoted cell death in AGS cells treated with cisplatin. Phospho-kinase array analysis revealed that cisplatin regulates intracellular p53 phosphorylation, while seselin regulates intracellular β-catenin expression by affecting the phosphorylation of glycogen synthase kinase-3 beta (GSK-3β), extracellular-signal-regulated kinase (ERK) and Src tyrosine kinase. Seselin and cisplatin promote the apoptosis of gastric cancer cells by the synergistic effect of two distinct signaling pathways. These findings suggest that seselin may be used as a complementary therapy to reduce the clinical dose of chemotherapy.https://doiserbia.nb.rs/img/doi/0354-4664/2023/0354-46642300023S.pdfseselincisplatincoumarinapoptosiscomplementary therapy |
| spellingShingle | Shyu Rong-Yaun Wang Chun-Hua Wu Chang-Chieh Wang Lu-Kai Tsai Fu-Ming Seselin promotes cisplatin-induced apoptosis of AGS gastric cancer cells by inhibiting β-catenin expression Archives of Biological Sciences seselin cisplatin coumarin apoptosis complementary therapy |
| title | Seselin promotes cisplatin-induced apoptosis of AGS gastric cancer cells by inhibiting β-catenin expression |
| title_full | Seselin promotes cisplatin-induced apoptosis of AGS gastric cancer cells by inhibiting β-catenin expression |
| title_fullStr | Seselin promotes cisplatin-induced apoptosis of AGS gastric cancer cells by inhibiting β-catenin expression |
| title_full_unstemmed | Seselin promotes cisplatin-induced apoptosis of AGS gastric cancer cells by inhibiting β-catenin expression |
| title_short | Seselin promotes cisplatin-induced apoptosis of AGS gastric cancer cells by inhibiting β-catenin expression |
| title_sort | seselin promotes cisplatin induced apoptosis of ags gastric cancer cells by inhibiting β catenin expression |
| topic | seselin cisplatin coumarin apoptosis complementary therapy |
| url | https://doiserbia.nb.rs/img/doi/0354-4664/2023/0354-46642300023S.pdf |
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