Effect of cultivation mode on the production of docosahexaenoic acid by Tisochrysis lutea
Abstract In this study, Tisochrysis lutea was cultivated in mixotrophic and heterotrophic cultures with glycerol as a carbon source and with glucose and acetate for comparison; autotrophic cultivation was the control group without a carbon source. It was found that T. lutea used glycerol and did not...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2018-03-01
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| Series: | AMB Express |
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| Online Access: | http://link.springer.com/article/10.1186/s13568-018-0580-9 |
| _version_ | 1828834581698052096 |
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| author | Hao Hu Lin-Lin Ma Xiao-Fei Shen Jia-Yun Li Hou-Feng Wang Raymond Jianxiong Zeng |
| author_facet | Hao Hu Lin-Lin Ma Xiao-Fei Shen Jia-Yun Li Hou-Feng Wang Raymond Jianxiong Zeng |
| author_sort | Hao Hu |
| collection | DOAJ |
| description | Abstract In this study, Tisochrysis lutea was cultivated in mixotrophic and heterotrophic cultures with glycerol as a carbon source and with glucose and acetate for comparison; autotrophic cultivation was the control group without a carbon source. It was found that T. lutea used glycerol and did not use glucose and acetate under mixotrophy. Mixotrophy slightly elevated the docosahexaenoic acid (DHA) and total fatty acids (TFA) content in the dry-weight and enhanced the DHA and TFA production in medium (41.3 and 31.9% respectively) at the end of a 16-day cultivation, while heterotrophy reduced the DHA content and TFA production. Under the mixotrophy, the glycerol contribution to the DHA production (16.19 mg/L) and the TFA production (97.8 mg/L) was not very high and the DHA yield [2.63% chemical oxygen demand (COD)] and TFA yield (13.1% COD) were also very low. Furthermore, T. lutea using glycerol had a period of adaptation, indicating that T. lutea was not an ideal microalga for organic carbon utilization. |
| first_indexed | 2024-12-12T17:42:17Z |
| format | Article |
| id | doaj.art-d78ae19f53e144cbaf573a4de3d256d4 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-12T17:42:17Z |
| publishDate | 2018-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-d78ae19f53e144cbaf573a4de3d256d42022-12-22T00:17:02ZengSpringerOpenAMB Express2191-08552018-03-018111210.1186/s13568-018-0580-9Effect of cultivation mode on the production of docosahexaenoic acid by Tisochrysis luteaHao Hu0Lin-Lin Ma1Xiao-Fei Shen2Jia-Yun Li3Hou-Feng Wang4Raymond Jianxiong Zeng5CAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of ChinaCAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of ChinaCAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of ChinaThe First Affiliated Hospital of Anhui University of Traditional Chinese MedicineCAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of ChinaCAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of ChinaAbstract In this study, Tisochrysis lutea was cultivated in mixotrophic and heterotrophic cultures with glycerol as a carbon source and with glucose and acetate for comparison; autotrophic cultivation was the control group without a carbon source. It was found that T. lutea used glycerol and did not use glucose and acetate under mixotrophy. Mixotrophy slightly elevated the docosahexaenoic acid (DHA) and total fatty acids (TFA) content in the dry-weight and enhanced the DHA and TFA production in medium (41.3 and 31.9% respectively) at the end of a 16-day cultivation, while heterotrophy reduced the DHA content and TFA production. Under the mixotrophy, the glycerol contribution to the DHA production (16.19 mg/L) and the TFA production (97.8 mg/L) was not very high and the DHA yield [2.63% chemical oxygen demand (COD)] and TFA yield (13.1% COD) were also very low. Furthermore, T. lutea using glycerol had a period of adaptation, indicating that T. lutea was not an ideal microalga for organic carbon utilization.http://link.springer.com/article/10.1186/s13568-018-0580-9Docosahexaenoic acid (DHA)Total fatty acids (TFAs)MixotrophyHeterotrophyTisochrysis lutea |
| spellingShingle | Hao Hu Lin-Lin Ma Xiao-Fei Shen Jia-Yun Li Hou-Feng Wang Raymond Jianxiong Zeng Effect of cultivation mode on the production of docosahexaenoic acid by Tisochrysis lutea AMB Express Docosahexaenoic acid (DHA) Total fatty acids (TFAs) Mixotrophy Heterotrophy Tisochrysis lutea |
| title | Effect of cultivation mode on the production of docosahexaenoic acid by Tisochrysis lutea |
| title_full | Effect of cultivation mode on the production of docosahexaenoic acid by Tisochrysis lutea |
| title_fullStr | Effect of cultivation mode on the production of docosahexaenoic acid by Tisochrysis lutea |
| title_full_unstemmed | Effect of cultivation mode on the production of docosahexaenoic acid by Tisochrysis lutea |
| title_short | Effect of cultivation mode on the production of docosahexaenoic acid by Tisochrysis lutea |
| title_sort | effect of cultivation mode on the production of docosahexaenoic acid by tisochrysis lutea |
| topic | Docosahexaenoic acid (DHA) Total fatty acids (TFAs) Mixotrophy Heterotrophy Tisochrysis lutea |
| url | http://link.springer.com/article/10.1186/s13568-018-0580-9 |
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